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A single-cell microbial fermentation system and method thereof

A microbial fermentation and fermentation method technology, applied in the field of biological fermentation equipment, can solve the problems of difficult pH control, long time-consuming, high cost, etc., and achieve the effect of easier pH control, saving time and cost, and avoiding waste

Active Publication Date: 2017-04-26
HUBEI GUANGJI PHARMA +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The invention provides a single-cell microbial fermentation system and a fermentation method thereof, which solves the problem that the pH value is not easy to control in the fermentation process of the existing device, and solves the problem of too many times of seed expansion in each round of fermentation in the existing fermentation method, which leads to time-consuming The problem of too long and high cost; to reduce the number of seed culture stages and cycles, optimize the addition of neutralizers, reduce the cost of seed expansion, and improve fermentation production efficiency

Method used

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  • A single-cell microbial fermentation system and method thereof
  • A single-cell microbial fermentation system and method thereof

Examples

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Embodiment 1

[0038] Embodiment 1, a single-cell microbial fermentation system, including a fermentation tank device 100 and a four-stage seed tank device 200, a fermentation tank device 100 and a four-stage seed tank device 200, each of which has one neutralizer storage tank;

[0039] The diameter of the culture tank of the fermentation tank device is 4.5m, and the volume is 200m 3 , The inner wall of the tank 111 is evenly distributed with temperature-controlled tubes, which are used to pass in the heating or cooling medium to raise or lower the temperature of the tank. The respirator 115 is a 0.2μm microporous filter; There are 4 agitators and 4 neutralizer distributors, the diameter of the closed circle constituting the neutralizer distributor is 1.125m, which is 1 / 4 of the diameter of the corresponding tank, and the inner diameter of the stainless steel tube is 30cm;

[0040] The cultivation tank of the first-stage seed tank device has a volume of 30L and a diameter of 0.3m. The side wall of...

Embodiment 2

[0044] Embodiment 2, a single-cell microbial fermentation system, including a fermentation tank device 100 and a three-stage seed tank device 200, each of which has two neutralizer storage tanks,

[0045] The capacity of the culture tank of the fermentation tank device is 300m 3 , The diameter is 5.8m, the inner wall of the tank 111 is evenly distributed with temperature-controlled tubes, which are used to pass in the heating medium or the cold medium to raise or lower the temperature of the tank. The respirator 115 is a 0.2μm microporous filter; There are 4 agitators and neutralizer distributors in the tank body 111. The closed circle forming the neutralizer distributor has a diameter of 4.35m, which is 3 / 4 of the diameter of the corresponding tank body. The inner diameter of the stainless steel pipe Is 30cm;

[0046] The culture tank of the first-stage seed tank device has a volume of 8L and a diameter of 0.2m. The side wall of the tank body has a surrounding hollow sandwich laye...

Embodiment 3

[0049] Example 3, using the system of Example 1, a fermentation method using lactic acid bacteria for lactic acid fermentation production includes the following steps:

[0050] A. Pre-installation steps: Before starting the system, prepare 50% calcium hydroxide emulsion in the neutralizer storage tanks of the fermentation tank device 100 and the seed tank device 200 at all levels for standby, and sterilize each neutralizer feeding tube; Fill the culture tank of the first-level seed tank device 200 with fresh culture medium and sterilize it; proceed to step B;

[0051] B. Seed expansion step: use thermostable anaerobic lactic acid bacteria (Lactobacillus casei B9232) to carry out 1.6L seed culture in a shaker bottle, about 15 hours to reach the logarithmic phase, complete seed culture, and transplant the seeds to the first The culture tank body of the first-level seed tank device;

[0052] Turn on the circulating pump corresponding to the first-stage seed tank device to make the neut...

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Abstract

The invention relates to a unicellular microorganism fermentation system and method and belongs to organism fermentation equipment and methods. The unicellular microorganism fermentation system and method solve the problem that the pH value is not easy to control in the fermentation process in an existing device and the problems that due to too many culture expanding times of seeds in each round of fermentation, too long time is consumed, and cost is too high in an existing fermentation device. The fermentation system comprises a fermentation tank device and N levels of seed tank devices. The fermentation tank device comprises a culture tank, a neutralizer storage jar, a circulating pump and a programmable logic controller. The constitution and structure of all the levels of seed tank devices are identical with those of the fermentation tank device, and only the dimension of the all the levels of seed tank devices is different from that of the fermentation tank device. The fermentation method comprises the steps of pre-loading, seed culture expanding, temporary seed storage, fermentation, and the Nth level seed re-culture and circulation. By means of the unicellular microorganism fermentation system and method, the seed culture expanding time is shortened, contamination risks are lowered and the time and cost for fermentation production are greatly saved; besides, the adding precision of a neutralizer is improved, a pipeline is prevented from scaling and then being blocked, the pH value is easier to control, and the level of fermentation production is increased.

Description

Technical field [0001] The invention belongs to biological fermentation equipment and methods, and specifically relates to a single-cell microbial fermentation system and a fermentation method thereof. Background technique [0002] Many important products, including various feeds, food additives, and raw materials, are mainly obtained through fermentation. Many single-celled microorganisms, such as bacteria and yeast, are used as production bacteria, and generally do not need to pass through spores. The fermentation cycle is short, the frequency of tank placement is high, and the seeds need to be expanded at a high frequency, which requires a large workload; On the one hand, with the development of fermentation technology, the volume of the fermentation tank gradually expanded to 200m 3 ~500m 3 , The number of seed tanks has gradually increased. Starting from shake flasks and finally to large-scale fermentation tanks, seed expansion requires 3 to 4 levels. The higher the number ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M1/36C12M1/34C12M1/04C12M1/02C12N1/18C12P7/56C12P25/00C12R1/125C12R1/865C12R1/245
CPCC12M23/58C12M27/02C12M29/00C12M29/06C12M29/18C12M41/22C12M41/26C12N1/18C12P7/56C12P25/00
Inventor 卢正东龚道夷陈情夏炜铠张磊余龙江
Owner HUBEI GUANGJI PHARMA
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