A single-cell microbial fermentation system and method thereof
A microbial fermentation and fermentation method technology, applied in the field of biological fermentation equipment, can solve the problems of difficult pH control, long time-consuming, high cost, etc., and achieve the effect of easier pH control, saving time and cost, and avoiding waste
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Embodiment 1
[0038] Embodiment 1, a single-cell microbial fermentation system, including a fermentation tank device 100 and a four-stage seed tank device 200, a fermentation tank device 100 and a four-stage seed tank device 200, each of which has one neutralizer storage tank;
[0039] The diameter of the culture tank of the fermentation tank device is 4.5m, and the volume is 200m 3 , The inner wall of the tank 111 is evenly distributed with temperature-controlled tubes, which are used to pass in the heating or cooling medium to raise or lower the temperature of the tank. The respirator 115 is a 0.2μm microporous filter; There are 4 agitators and 4 neutralizer distributors, the diameter of the closed circle constituting the neutralizer distributor is 1.125m, which is 1 / 4 of the diameter of the corresponding tank, and the inner diameter of the stainless steel tube is 30cm;
[0040] The cultivation tank of the first-stage seed tank device has a volume of 30L and a diameter of 0.3m. The side wall of...
Embodiment 2
[0044] Embodiment 2, a single-cell microbial fermentation system, including a fermentation tank device 100 and a three-stage seed tank device 200, each of which has two neutralizer storage tanks,
[0045] The capacity of the culture tank of the fermentation tank device is 300m 3 , The diameter is 5.8m, the inner wall of the tank 111 is evenly distributed with temperature-controlled tubes, which are used to pass in the heating medium or the cold medium to raise or lower the temperature of the tank. The respirator 115 is a 0.2μm microporous filter; There are 4 agitators and neutralizer distributors in the tank body 111. The closed circle forming the neutralizer distributor has a diameter of 4.35m, which is 3 / 4 of the diameter of the corresponding tank body. The inner diameter of the stainless steel pipe Is 30cm;
[0046] The culture tank of the first-stage seed tank device has a volume of 8L and a diameter of 0.2m. The side wall of the tank body has a surrounding hollow sandwich laye...
Embodiment 3
[0049] Example 3, using the system of Example 1, a fermentation method using lactic acid bacteria for lactic acid fermentation production includes the following steps:
[0050] A. Pre-installation steps: Before starting the system, prepare 50% calcium hydroxide emulsion in the neutralizer storage tanks of the fermentation tank device 100 and the seed tank device 200 at all levels for standby, and sterilize each neutralizer feeding tube; Fill the culture tank of the first-level seed tank device 200 with fresh culture medium and sterilize it; proceed to step B;
[0051] B. Seed expansion step: use thermostable anaerobic lactic acid bacteria (Lactobacillus casei B9232) to carry out 1.6L seed culture in a shaker bottle, about 15 hours to reach the logarithmic phase, complete seed culture, and transplant the seeds to the first The culture tank body of the first-level seed tank device;
[0052] Turn on the circulating pump corresponding to the first-stage seed tank device to make the neut...
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Abstract
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