Bumetanide medicine composition and anti-inflammatory and abirritation effects thereof

An anti-inflammatory and analgesic and composition technology, which is applied in the directions of drug combinations, anti-inflammatory agents, and medical preparations containing active ingredients, can solve problems such as bumetanide that have not been reported yet, and achieve improved anti-inflammatory and analgesic effects. , significant progress, highlighting the effect of substantive characteristics

Inactive Publication Date: 2016-08-10
薛君
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there is no relevant report on bumetanide an

Method used

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  • Bumetanide medicine composition and anti-inflammatory and abirritation effects thereof
  • Bumetanide medicine composition and anti-inflammatory and abirritation effects thereof
  • Bumetanide medicine composition and anti-inflammatory and abirritation effects thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Example 1: Compound (I) Separation Preparation and Structure Confirmation

[0019] Sources of reagents: ethanol, petroleum ether, ethyl acetate, n-butanol, and dichloromethane were of analytical grade, purchased from Shanghai Lingfeng Chemical Reagent Co., Ltd. Methanol, of analytical grade, were purchased from Jiangsu Hanbang Chemical Reagent Co., Ltd.

[0020] Separation method: (a) Grind the dried rhizome (5kg) and extract with 75% ethanol under heat reflux (20L×3 times), combine the extracts, concentrate until no alcohol smell (4L), and then use petroleum ether (4L×3 times) 3 times), ethyl acetate (4L × 3 times) and water-saturated n-butanol (4L × 3 times) were extracted to obtain petroleum ether extract, ethyl acetate extract and n-butanol extract respectively; (b) step (a) The n-butanol extract was removed with AB-8 macroporous resin, first eluted with 8% ethanol for 10 column volumes, then with 70% ethanol for 12 column volumes, and collected 70% eluate, Concent...

Embodiment 2

[0023] Embodiment 2: Anti-inflammatory and analgesic effects

[0024] 1. Materials and methods

[0025] 1.1 Drugs and reagents

[0026] Bumetanide was purchased from China National Institute for the Control of Pharmaceutical and Biological Products. Compound (I) is self-made, and the preparation method is shown in Example 1. Aspirin enteric-coated tablets (Jinan Yongning Pharmaceutical Co., Ltd.), Tween 80 (Tianjin Guangcheng Chemical Reagent Co., Ltd.); xylene (Tianjin Botong Chemical Co., Ltd.); Evans Blue (Sinopharm Chemical Reagent Co., Ltd.).

[0027] 1.2 Animals

[0028] Kunming mice, weighing 18-22 g, half male and half male, clean grade, provided by the Experimental Animal Center of Taishan Medical College.

[0029] 1.3 Analgesic experiment: mouse writhing method

[0030] Take 50 mice, half male and half female, weighing 18-22 g, and randomly divide them into bumetanide group (0.2 g·kg -1 ), compound (I) group (0.2g·kg -1 ), bumetanide and compound (I) compositi...

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Abstract

The invention discloses a bumetanide medicine composition and anti-inflammatory and abirritation effects thereof. The bumetanide medicine composition includes bumetanide and a natural product compound (I) which has a novel structure and is extracted from dry rhizome of belamcanda chinensis. The bumetanide and the compound (I) are not so excellent in anti-inflammatory and abirritation effects when being used separately but are significantly improved in the anti-inflammatory and abirritation effects when being used together, so that the composition can be developed to prepare anti-inflammatory and abirritation drugs. Compared with the prior art, the composition has outstanding substantive features and significant improvement.

Description

technical field [0001] The invention belongs to the field of biomedicine and relates to a new application of bumetanide, in particular to a pharmaceutical composition of bumetanide and its anti-inflammatory and analgesic effects. Background technique [0002] Bumetanide, also known as butoxybenzoic acid, is a loop diuretic. Its site of action, mechanism of action, electrolyte loss, and action characteristics are similar to those of furosemide and ethacrynic acid. It has the characteristics of high efficiency, quick-acting, short-acting and low toxicity. [0003] The effect of bumetanide on water and electrolyte excretion is basically the same as that of furosemide, and its diuretic effect is 20 to 60 times that of furosemide. It mainly inhibits the active reabsorption of NaCl in the thick-walled segment of the ascending branch of the loop of Henle of the renal tubule, and also inhibits the reabsorption of Na+ in the proximal tubule, but has no effect on the distal tubule, s...

Claims

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Application Information

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IPC IPC(8): C07D491/22A61K31/439A61P29/00A61K31/196
CPCA61K31/196A61K31/439C07D491/22A61K2300/00
Inventor 薛君李丽赵玉强
Owner 薛君
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