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Single domain antibody and its coding gene, immunotoxin and its coding gene, preparation method, expression vector, application, and host cell

A single-domain antibody, immunotoxin technology, applied in the direction of anti-receptor/cell surface antigen/cell surface determinant immunoglobulin, botanical equipment and methods, biochemical equipment and methods, etc. It is difficult to guarantee the homogeneity of the connection, and it is easy to produce immunogenicity, etc., to achieve the effects of low cost, efficient targeted killing of tumor cells, and simple preparation process.

Inactive Publication Date: 2019-02-26
PEKING UNIV SHENZHEN GRADUATE SCHOOL
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The disadvantage of this technology is that in addition to the disadvantages of monoclonal antibody preparation itself, it is difficult to ensure the uniformity of chemical cross-linking, and the toxin has a long half-life in vivo, which is prone to immunogenicity

Method used

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  • Single domain antibody and its coding gene, immunotoxin and its coding gene, preparation method, expression vector, application, and host cell
  • Single domain antibody and its coding gene, immunotoxin and its coding gene, preparation method, expression vector, application, and host cell
  • Single domain antibody and its coding gene, immunotoxin and its coding gene, preparation method, expression vector, application, and host cell

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preparation example Construction

[0048] Yet another embodiment of the present invention is as follows: a method for preparing an immunotoxin of CD38, which includes the following steps: combining the CD38 single domain antibody gene sequence shown in SEQ ID No.1 in the sequence listing with Pseudomonas aeruginosa The gene sequence of the toxin mutant PE38 is cloned into an expression vector, and then transformed into an expression host strain, the host strain is expanded and cultured to induce expression, and the immunotoxin is obtained after purification. That is, the preparation method first clones the CD38 single-domain antibody gene sequence shown in SEQ ID No.1 in the sequence table and the gene sequence of the Pseudomonas aeruginosa exotoxin mutant PE38 into an expression vector, and then transforms it into an expression host strain Here, the host strain is expanded and cultured to induce expression, and the immunotoxin is obtained after purification. For example, the CD38 single-domain antibody 1053 ge...

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Abstract

The invention discloses a CD 38 single-domain antibody and its coding gene, a CD38 immunotoxin and, preparation method, expression vector and use in a drug for treating multiple myeloma and chronic lymphoid leukemia and a host cell. Through a single-domain antibody-immunotoxin fusion technology, the CD38 immunotoxin is obtained. The CD38 immunotoxin has the advantages of simple processes, low cost and high tumor cell targeting killing efficiency and is based on the CD 38 single-domain antibody. The CD38 immunotoxin is conducive to multiple myeloma and chronic lymphoid leukemia treatment.

Description

technical field [0001] The present invention relates to the research of CD38, in particular, a CD38 single domain antibody and its coding gene, CD38 immunotoxin with the CD38 single domain antibody and its preparation method, and the expression vector of the immunotoxin, the immunotoxin The application in preparation of medicine for treating multiple myeloma, the application of the immunotoxin in preparation of medicine for treatment of chronic lymphoid leukemia, and the host cell with the expression vector. Background technique [0002] In the 1980s, CD38 was discovered on the surface of T lymphocytes and used as a marker of cell differentiation. In 2004, it was found that CD38 is generally highly expressed in multiple myeloma cells, while it is in a low expression state on normal lymphocytes, bone marrow cells and some non-hematopoietic cells, which makes CD38 an ideal target for the treatment of myeloma. Currently, several anti-CD38 monoclonal antibodies are in clinical ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K16/28C07K19/00C12N15/31C12N15/13C12N15/62C12N15/63C12N1/21A61K39/395A61P35/00A61P35/02
Inventor 赵永娟李汉璋黎婷
Owner PEKING UNIV SHENZHEN GRADUATE SCHOOL
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