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Method for identifying natural taurine and synthetic taurine

A taurine, natural technology, applied in the direction of instruments, measuring devices, scientific instruments, etc., can solve the problems of endangering human health, interference of natural taurine, and identification of natural taurine, etc. Effect

Active Publication Date: 2016-08-10
JIMEI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because natural taurine comes from natural organisms, it is almost harmless to human health, while synthetic taurine is potentially harmful to human health due to its raw materials and chemical intermediate products, the market price of natural taurine is higher than that of synthetic taurine About 100 times, some unscrupulous traders pretended to be natural taurine with synthetic taurine to make huge profits. At present, there is no method and quality system to identify natural taurine and synthetic taurine in the technology, so the natural taurine market has suffered serious Interference and Unfair Competition

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Embodiment 1: the identification of natural taurine and synthetic taurine

[0024] (1) Sample to be tested

[0025] The sample ① purchased from the market produced by Qianjiang Yongan Pharmaceutical Co., Ltd. contains 98.5-101.5 dry weight % of taurine, and the taurine content test method adopts the taurine content determination method in GB 14759-2010;

[0026] The sample ② was extracted from abalone viscera according to the method of invention patent ZL201310283654.5, and contained 98.5-101.5% by dry weight of taurine. The taurine content test method used the taurine content determination method in GB 14759-2010.

[0027] (2) Preparation of sample: Dissolve sample ① and sample ② in ultrapure water respectively to make a solution with a concentration of 50 mg / mL;

[0028] (3) Detection of samples:

[0029] a. Mix equal volumes of taurine solution and scintillation fluid evenly to prepare sample solution to be tested;

[0030] b. Using a low-background liquid scintil...

Embodiment 2

[0033] Embodiment 2: the identification of natural taurine and synthetic taurine

[0034] (1) Sample to be tested

[0035] The sample ① purchased from the market contains 98.5-101.5 dry weight % taurine produced by Jiangsu Yuanyang Pharmaceutical Co., Ltd., and the taurine content test method adopts the taurine content determination method in GB 14759-2010;

[0036] The sample ② was extracted from variegated clams according to the method of invention patent ZL201310283931.2, and contained 98.5-101.5% by dry weight of taurine.

[0037] (2) Sample preparation: Dissolve sample ① and sample ② with ultrapure water and dilute to make a solution with a concentration of 20 mg / mL;

[0038] (3) Detection of samples:

[0039] a. Mix equal volumes of taurine solution and scintillation fluid evenly to prepare sample solution to be tested;

[0040] b. Using a low-background liquid scintillation analyzer 14 The C channel measures the background (ultrapure water) and the CPM value of the ...

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Abstract

The invention discloses a method for identifying natural taurine and synthetic taurine. Characteristics that unstable isotope <14>C activity of the natural taurine is relatively high and the unstable isotope <14>C activity of the synthetic taurine is quite low are used. Firstly, a to-be-measured taurine sample is dissolved with ultra-pure water, and then is diluted into a to-be-measured taurine solution; then the to-be-measured taurine solution and a scintillation fluid are mixed evenly with equal volume, to prepare a to-be-measured sample solution; the CPM values of a background and the to-be-measured sample solution are detected by a <14>C channel of a low background liquid scintillation analyzer; and according to the measured CPM values, a result is judged. The method is simple and accurate.

Description

technical field [0001] The invention relates to the field of detection methods of marine active substances, in particular to a method for identifying natural taurine and synthetic taurine. Background technique [0002] Taurine is a sulfur-containing non-protein amino acid, which was first isolated from ox bile. It exists in a free state in the body and does not participate in the biosynthesis of proteins in the body. It is a conditionally essential amino acid that is mainly supplied by food. Because it has the functions of regulating kidney function, anti-oxidation, promoting brain development and enhancing eyesight, it is widely used in various industries such as medicine, functional food and feed. [0003] At present, the production methods of taurine mainly include chemical synthesis and natural extraction. The chemical synthesis method uses poisonous ethylene oxide or 2-aminoethanol as raw materials to obtain it through chemical synthesis; the natural extraction method ...

Claims

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Application Information

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IPC IPC(8): G01N23/00
CPCG01N23/00
Inventor 章骞曹敏杰林峰崔璨蔡秋凤
Owner JIMEI UNIV
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