New stevia rebaudiana variety 817096 Puxing No.5 and preparation of high-RD-content stevioside

A technology of steviol glycosides and stevia, which is applied in the preparation of sugar derivatives, sugar derivatives, sugar derivatives, etc., can solve the problems of not being able to meet customer needs, unfavorable cost control, and low content of stevia

Active Publication Date: 2016-08-17
PURECIRCLE AMERICAN CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Due to the low content of RD in Stevia rebaudiana, in the past, the only way to obtain high-purity RD was repeated recrystallization. The disadvantage of repeated recrystallization is that the product yield is low, which is not conducive to cost control; Large-scale production, unable to meet customer needs

Method used

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  • New stevia rebaudiana variety 817096 Puxing No.5 and preparation of high-RD-content stevioside
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  • New stevia rebaudiana variety 817096 Puxing No.5 and preparation of high-RD-content stevioside

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Experimental program
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Effect test

preparation example Construction

[0023] 2. Preparation of high RD content steviol glycoside products

[0024] (a) Pretreatment: The crushed dried leaves of 817096PC Star 5 (817096PC Star 5) are extracted in hot water to prepare a steviol glycoside extract. The extract is subjected to two processes of flocculation precipitation and plate and frame pressure filtration. , Enter the next process;

[0025] (b) One-time adsorption and desorption: Adsorb the effective components of steviol glycosides in the above-mentioned extract with a resin until the resin is sweet; wash the resin with dilute sodium hydroxide solution, dilute hydrochloric acid solution, and pure water until the pH of the effluent reaches 7 o'clock Column desorption of the resin with ethanol solution to obtain a desorption solution with an RD of 22-25% and a glycoside content of 78-86%. The desorption solution enters the next process;

[0026] (c) Primary ion exchange: After the analytical solution passes through the primary ion exchange to remove impur...

Embodiment 1

[0035] Parental traits used for cross breeding:

[0036] Female parent AKH / G.8.D: A new high-Stev-content stevia variety introduced from Paraguay in 2010. The height of the plant is 50-55cm, the leaves are oval, the edges of the leaves have no obvious serrations, and the color is dark green. The content of RebA in dry leaves is 0.00%, and the content of St is 18.00%.

[0037] Male parent AKH L1: A new stevia variety with high Reb A content introduced from Paraguay in 2010. This variety is a late-maturing variety, with a plant height of 51-70 cm. It grows slowly in the early stage of field planting, and grows vigorously in the middle and late stages. The field growth period is 135-150 days. The leaf color is light green / yellow green. The content of Reb A in dry leaves is 11.5%, and the content of St is 1.30%. The yield of dry leaves per mu is more than 200 kg.

[0038] The second female parent AKH / G.8.D: A new high-Stev content (hereinafter referred to as St) stevia variety introd...

Embodiment 2

[0068] Preparation of high RD content steviol glycoside products

[0069] (a) Pretreatment: Mix stevia leaves with 50°C soft water at a weight ratio of 1:15 to prepare a steviol glycoside extract. After flocculation and sedimentation and plate and frame filtration, the filtrate after plate and frame filtration enters the next process. Process

[0070] (b) Primary adsorption and desorption: Use primary adsorption resin to adsorb the effective components of steviol glycosides in the above-mentioned extract, treat the resin that adsorbs the effective components of steviol glycosides with sodium hydroxide solution and hydrochloric acid solution, and rinse the resin with pure water to the pH of the effluent. When the value reaches 7 o'clock, use ethanol solution for desorption to obtain a desorption solution with an RD of 22.69% and a glycoside content of 79.5%. The desorption solution is de-alcoholized and enters the next process;

[0071] (c) Primary ion exchange: the de-alcohol desorp...

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Abstract

The invention provides a stevia rebaudiana variant plant which is 817096 Puxing No.5, a callus tissue is preserved by China General Microbiological Culture Collection Center (CGMCC) and has the preservation number of CGMCC No.9703. The invention also provides a high-RD-content stevioside product, wherein the RD / TSG ratio is greater than 0.28.

Description

Technical field [0001] The invention relates to a new variety of stevia which contains high content of RD and can be produced by continuous cultivation of preserved callus; it relates to a method for preparing high RD content steviol glycosides from dried leaves of stevia of this variety. Background technique [0002] Stevia, whose scientific name is Stevia Rebaudiana Bertoni, is a perennial herbaceous plant in the Compositae family, native to Paraguay, South America. Its leaves contain steviol glycosides, and its sweetness is 150 to 300 times that of sucrose. A new type of natural sweetener with sweetness, low heat energy, good taste, safe and non-toxic, can be widely used in food, beverage, medicine, daily chemical industry and other industries. [0003] Steviol glycosides are multi-component glycoside mixtures. The glycoside components and content determine the sweetness and taste of the mixture. The steviol glycosides in stevia contain 13 components, namely Rebaudioside A (Reb...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02C07H15/256C07H1/08A01H5/12
CPCC12Q1/6895C12Q2600/13A01H5/12A01H6/1488
Inventor 奥维迪·玛珂善李善汪卜宇成
Owner PURECIRCLE AMERICAN CORP
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