Wolfberry anther induction medium and anther breeding method

A technology for inducing medium and Lycium barbarum flowers, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of low induction rate of embryoid bodies, limiting the application and development of Lycium barbarum anthers, and imperfect flower cultivation techniques, etc. Achieve the effects of improving the callus induction rate of anthers, high callus quality and speeding up the process

Inactive Publication Date: 2016-08-17
宁夏中宁枸杞产业创新研究院有限公司
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Problems solved by technology

[0006] At present, although considerable progress has been made in the culture of wolfberry anthers, in general, the culture technology of wolfberry anthers still has the defects of low embryoid body induction rate and imperfect flower culture technology, which limits the application of wolfberry anther culture in genetics and breeding. application and development in

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  • Wolfberry anther induction medium and anther breeding method

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Embodiment

[0032] Prepare the medium provided by the present invention:

[0033] In 2014, the wolfberry varieties Ningqi No. 3 and Huangguo Lycium barbarum were selected as anther donors for anther culture, and the wolfberry induction medium of the present invention was prepared, and the flower breeding method of the present invention was used to cultivate anthers. The specific steps were as follows:

[0034] (1) Bud collection and low temperature treatment

[0035] When Ningqi No. 3 and Huangguo Lycium barbarum conventionally cultivated in the field are in the early flowering stage or the full flowering stage, the flower buds that are in the middle and late stage of mononuclear development stage are collected from 9:00 am to 11:00 am. Wrap the collected flower buds with wet gauze and then wrap them in a self-sealing plastic bag, and place them in a refrigerator at 4°C for 2-7 days;

[0036] (2) Preparation of culture medium

[0037] According to the best recipe: KNO 3 1000mg / L, NH ...

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Abstract

The invention discloses a wolfberry anther induction medium and an anther breeding method and belongs to the field of tissue culture of wolfberry. The wolfberry anther induction medium contains major elements, minor elements, iron salt and complex agent, organic ingredients, inorganic additive, plant growth regulator, physiological activator, carbon source, coagulator and other additives. The wolfberry anther breeding method includes steps of bud taking and low-temperature treatment, preparation of the medium, anther inoculation, temperature-variable induction culture, differentiation and rooting, domestication and transplanting. By adopting the wolfberry anther induction medium and the anther breeding method for induction culture of wolfberry anther, anther callus induction rate can be increased effectively, obtained wolfberry anther callus is high in quality, and a lot of high-quality test tube seedlings can be formed in a short time, so that wolfberry breeding process is accelerated greatly. The wolfberry anther induction medium and the anther breeding method can be used for wolfberry anther breeding and can also be used for the field of wolfberry in-vitro rapid propagation and germplasm preservation.

Description

technical field [0001] The invention relates to a wolfberry anther induction medium and a flower cultivation method, in particular to a wolfberry anther induction medium and a flower cultivation method which can effectively improve the induction rate of wolfberry anther callus and have good flower cultivation quality, and belong to the field of wolfberry The field of tissue culture. Background technique [0002] wolfberry ( Lycium Barbarum . L), for Solanaceae ( Solanaceae ) Lycium barbarum ( Lycium ) plants, there are about 80 species in the world, widely distributed in North and South America, Eurasia, Africa and Pacific Islands. There are 7 species and 2 varieties of Lycium barbarum recorded in my country, namely Lycium barbarum and its variants Northern Lycium barbarum, Ningxia Lycium barbarum and its variants Huangguo Lycium barbarum, Xinjiang Lycium barbarum, Black fruit Lycium barbarum, Calyx Lycium barbarum, Yunnan Lycium barbarum, Cylindrical Lycium barbarum. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 张瑞明宋晓燕潭江
Owner 宁夏中宁枸杞产业创新研究院有限公司
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