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Eucommia ulmoides extract as well as preparation method and application thereof

An extract, ethanol reflux extraction technology, applied in the preparation of sugar derivatives, chemical instruments and methods, sugar derivatives, etc., can solve the problem of no anti-inflammatory application of Eucommia ulmoides, and achieve the effect of obvious anti-inflammatory activity

Inactive Publication Date: 2016-08-17
JIANGXI POZIN PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no report on the anti-inflammatory application of Eucommia

Method used

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  • Eucommia ulmoides extract as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Example 1 Preparation of Eucommia Extract One.

[0016] Pulverize 20kg of Eucommia medicinal materials, add 220L of 95% ethanol, heat and reflux extraction twice, each time for 2 hours, and filter; add 80% ethanol to 200L reflux extraction once, extract for 2 hours, and filter; combine Eucommia alcohol for 3 times Extract liquid, recover ethanol and concentrate to contain 0.6g crude drug·ml -1 The liquid ointment; the ointment was allowed to stand for 12 hours, centrifuged, and the supernatant was taken; the supernatant was dissolved in 1.2mlmin -1 Speed ​​through HPD 100 macroporous resin; sample volume does not exceed 2.0g crude drug·ml -1 The resin was eluted with 6 times column volume of purified water, 10 times column volume of 20% ethanol, discarded, and then eluted with 10 times column volume of 90% ethanol, and the 90% ethanol eluted part was collected, and the eluent was recovered under reduced pressure Solvent, dry at 60°C. Pulverize to obtain 0.32kg of euc...

Embodiment 2

[0018] Example 2 Preparation of Eucommia Extract II.

[0019] 100kg of eucommia medicinal materials, 1000L of 90% ethanol was added, heated and refluxed twice, 2 hours each time, and filtered; the medicinal residue was added with 70% ethanol and 800L reflux extracted once, extracted for 2 hours, filtered; combined 3 times of Eucommia ethanol extraction liquid, recovered ethanol and concentrated to contain 0.5g crude drug·ml -1 The liquid ointment; the ointment was allowed to stand for 12 hours, centrifuged, and the supernatant was taken; the supernatant was dissolved in 1.0mlmin -1 The speed passes through the HPD100 macroporous resin; the loading amount does not exceed 2.0g crude drug·ml -1 The resin was eluted with 5 times column volume of purified water, 8 times column volume of 20% ethanol, discarded, and then 8 times column volume of 90% ethanol was used to collect the 90% ethanol eluted part, and the eluent was recovered under reduced pressure Solvent, dry at 60°C. Pu...

Embodiment 3

[0021] Example 3 Application of Eucommia extract in anti-inflammation.

[0022] 60 male Kunming mice were randomly divided into negative control group (0.6% CMC-Na solution), positive control group (aspirin 100mgkg -1 , Celecoxib 5mgkg -1 ), Eucommia extract high, medium and low dose groups (400mgkg -1 , 200mgkg -1 , 100mgkg -1 ) 10 per group. Animals in each group were administered intragastrically once a day for 3 consecutive days. 30 minutes after the last administration, 0.1ml of xylene was evenly applied to the front and back of the left ear, and the right ear was used as a control. The mice were killed 20 minutes after the inflammation was induced, and the two ears were cut off along the pinna line, and the ear pieces were respectively punched at the same position with a stainless steel puncher with a diameter of 8 mm, and weighed. The swelling degree was defined as the difference in mass between the left and right ear pieces.

[0023] Inhibition rate of auricle swe...

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PUM

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Abstract

An extract of eucommia ulmoides and its preparation method and application belong to the technical field of traditional Chinese medicine. The Eucommia ulmoides extract contains olivine 9-glucoside, olivine 4'-glucoside and arctiin, and their mass percentages are respectively 3.10-3.70%, 1.40-1.70%, and 9.50-10.50%. It is prepared by pulverizing Eucommia ulmoides, extracting, concentrating, centrifuging, standing, and separating with macroporous adsorption resin. Has a significant anti-inflammatory effect.

Description

technical field [0001] The invention belongs to the technical field of traditional Chinese medicines, in particular to an extract of Eucommia ulmoides and its preparation method and application. Background technique [0002] Eucommia ulmoides Oliv. is the dry bark of the Eucommia ulmoides plant, which is a traditional and precious nourishing Chinese medicine in my country. It is used to nourish the liver and kidney, and strengthen the bones and muscles. Modern pharmacological studies have found that Eucommia has a variety of functional activities, such as: lowering blood pressure, anti-aging, anti-cancer, antibacterial, analgesic, anti-inflammatory, diuretic, sedative, calming, anti-viral, enhancing the body's non-specific immunity, and It can carry out biphasic regulation and other effects on cellular immunity, and also has certain effects on the nerve center. [0003] The chemical constituents in Eucommia that have been reported in the literature include about 200 chemica...

Claims

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Application Information

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IPC IPC(8): C07H15/18C07H15/26C07H1/08A61P29/00
CPCC07H15/18C07H1/08C07H15/26
Inventor 康兴东肖军平吴永忠张荷英刘恩位毛金娣
Owner JIANGXI POZIN PHARMA
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