Eucommia ulmoides extract as well as preparation method and application thereof
An extract, ethanol reflux extraction technology, applied in the preparation of sugar derivatives, chemical instruments and methods, sugar derivatives, etc., can solve the problem of no anti-inflammatory application of Eucommia ulmoides, and achieve the effect of obvious anti-inflammatory activity
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Embodiment 1
[0015] Example 1 Preparation of Eucommia Extract One.
[0016] Pulverize 20kg of Eucommia medicinal materials, add 220L of 95% ethanol, heat and reflux extraction twice, each time for 2 hours, and filter; add 80% ethanol to 200L reflux extraction once, extract for 2 hours, and filter; combine Eucommia alcohol for 3 times Extract liquid, recover ethanol and concentrate to contain 0.6g crude drug·ml -1 The liquid ointment; the ointment was allowed to stand for 12 hours, centrifuged, and the supernatant was taken; the supernatant was dissolved in 1.2mlmin -1 Speed through HPD 100 macroporous resin; sample volume does not exceed 2.0g crude drug·ml -1 The resin was eluted with 6 times column volume of purified water, 10 times column volume of 20% ethanol, discarded, and then eluted with 10 times column volume of 90% ethanol, and the 90% ethanol eluted part was collected, and the eluent was recovered under reduced pressure Solvent, dry at 60°C. Pulverize to obtain 0.32kg of euc...
Embodiment 2
[0018] Example 2 Preparation of Eucommia Extract II.
[0019] 100kg of eucommia medicinal materials, 1000L of 90% ethanol was added, heated and refluxed twice, 2 hours each time, and filtered; the medicinal residue was added with 70% ethanol and 800L reflux extracted once, extracted for 2 hours, filtered; combined 3 times of Eucommia ethanol extraction liquid, recovered ethanol and concentrated to contain 0.5g crude drug·ml -1 The liquid ointment; the ointment was allowed to stand for 12 hours, centrifuged, and the supernatant was taken; the supernatant was dissolved in 1.0mlmin -1 The speed passes through the HPD100 macroporous resin; the loading amount does not exceed 2.0g crude drug·ml -1 The resin was eluted with 5 times column volume of purified water, 8 times column volume of 20% ethanol, discarded, and then 8 times column volume of 90% ethanol was used to collect the 90% ethanol eluted part, and the eluent was recovered under reduced pressure Solvent, dry at 60°C. Pu...
Embodiment 3
[0021] Example 3 Application of Eucommia extract in anti-inflammation.
[0022] 60 male Kunming mice were randomly divided into negative control group (0.6% CMC-Na solution), positive control group (aspirin 100mgkg -1 , Celecoxib 5mgkg -1 ), Eucommia extract high, medium and low dose groups (400mgkg -1 , 200mgkg -1 , 100mgkg -1 ) 10 per group. Animals in each group were administered intragastrically once a day for 3 consecutive days. 30 minutes after the last administration, 0.1ml of xylene was evenly applied to the front and back of the left ear, and the right ear was used as a control. The mice were killed 20 minutes after the inflammation was induced, and the two ears were cut off along the pinna line, and the ear pieces were respectively punched at the same position with a stainless steel puncher with a diameter of 8 mm, and weighed. The swelling degree was defined as the difference in mass between the left and right ear pieces.
[0023] Inhibition rate of auricle swe...
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