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A pcr thermocycling device

A thermal cycle device and heat dissipation device technology, which is applied in biochemical cleaning devices, enzymology/microbiology devices, heating or cooling equipment, etc., can solve the problems of high processing technology requirements, uneven heating of samples, and high production and processing costs. Achieve the effect of improving uniformity, simple and reasonable structure, and convenient production and processing

Active Publication Date: 2018-06-12
成都瀚辰光翼科技有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, because the contact surface between the metal plate and the reagent tube in the conventional metal bath is not flat, it is easy to produce uneven heating of the sample, which requires high processing technology for the reagent tube, metal plate, etc., and the production and processing costs are high.

Method used

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  • A pcr thermocycling device
  • A pcr thermocycling device
  • A pcr thermocycling device

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] like Figure 1 to Figure 4 As shown, a PCR thermal cycle device includes a sample packaging tray, the sample packaging tray includes an upper heat insulating layer 2 connected in sequence from top to bottom, a sample layer and a lower heat conducting layer 5, and the sample layer includes a wall plate 4 and a sample 3, Sample 3 is placed in the wall plate 4, and through holes are evenly arranged on the wall plate 4, and the hole array is arranged according to 384 holes. There is a concave platform, the concave platform is set between the two through holes, the upper heat insulating layer 2 and the lower heat conducting layer 5 cover the upper surface and the lower surface of the wall plate 4 respectively, the upper heat insulating layer 2, the lower heat conducting layer 5 and the side of the through hole The wall constitutes a complete sample container, and the upper heat insulating layer 2 and the lower heat conducting layer 5 are used as the upper cover and the botto...

Embodiment 2

[0051] like Figure 1 to Figure 4 As shown, a PCR thermal cycle device includes a sample packaging tray, the sample packaging tray includes an upper heat insulating layer 2 connected in sequence from top to bottom, a sample layer and a lower heat conducting layer 5, and the sample layer includes a wall plate 4 and a sample 3, Sample 3 is placed in the wall plate 4, and through holes are evenly arranged on the wall plate 4, and the hole array is arranged according to 384 holes. There is a concave platform, the concave platform is set between the two through holes, the upper heat insulating layer 2 and the lower heat conducting layer 5 cover the upper surface and the lower surface of the wall plate 4 respectively, the upper heat insulating layer 2, the lower heat conducting layer 5 and the side of the through hole The wall constitutes a complete sample container, and the upper heat insulating layer 2 and the lower heat conducting layer 5 are used as the upper cover and the botto...

Embodiment 3

[0058] like Figure 1 to Figure 4 As shown, a PCR thermal cycle device includes a sample packaging tray, the sample packaging tray includes an upper heat insulating layer 2 connected in sequence from top to bottom, a sample layer and a lower heat conducting layer 5, and the sample layer includes a wall plate 4 and a sample 3, Sample 3 is placed in the wall plate 4, and through holes are evenly arranged on the wall plate 4, and the hole array is arranged according to 384 holes. There is a concave platform, the concave platform is set between the two through holes, the upper heat insulating layer 2 and the lower heat conducting layer 5 cover the upper surface and the lower surface of the wall plate 4 respectively, the upper heat insulating layer 2, the lower heat conducting layer 5 and the side of the through hole The wall constitutes a complete sample container, and the upper heat insulating layer 2 and the lower heat conducting layer 5 are used as the upper cover and the botto...

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Abstract

The invention discloses a PCR heat cycle device, and belongs to the field of experiment consumable materials. The PCR heat cycle device disclosed by the invention comprises a sample packaging disc, wherein the sample packaging disc comprises an upper heat insulating layer, a sample layer and a lower heat conducting layer which are sequentially connected from top to bottom; the sample layer comprises a wallboard and samples; the samples are placed inside the wallboard. The PCR heat cycle device disclosed by the invention has the characteristics that the device is convenient for production processing, all the samples can be uniformly and quickly heated and cooled, the heating and cooling rates are improved, the size is small, and the device is convenient for use.

Description

technical field [0001] The invention relates to a thermal cycle device, in particular to a PCR thermal cycle device. Background technique [0002] PCR (polymerase chain reaction) is a molecular biological technique that can amplify specific DNA fragments outside the living body. This technology has been widely used in molecular diagnosis, precision medicine, modern agriculture, food safety and other fields. In most PCR reactions, it is necessary to periodically increase and decrease the temperature of the sample to achieve the amplification of specific DNA fragments. [0003] The prior art generally uses water baths or conventional metal baths to achieve thermal cycling. The water bath thermal cycle realizes the heating and cooling process of the sample by repeatedly immersing the sample in two constant temperature pools of high temperature and low temperature. However, since the water bath cycle needs to be equipped with a constant temperature pool and corresponding wate...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M1/38C12M1/00
CPCB01L3/50851B01L7/52
Inventor 张晗韦晨
Owner 成都瀚辰光翼科技有限责任公司
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