Capillary Electrophoresis Method for Separating Complexes in Two Online Reactions and Reverse Screening
A technology of capillary electrophoresis and reverse screening, which is applied in the fields of biochemical equipment and methods, measurement/inspection of microorganisms, DNA preparation, etc. It can solve the problems of large amount of target molecules, cumbersome optimization of reaction conditions, time-consuming and labor-intensive reverse screening steps, etc. problems, to achieve the effect of eliminating incubation steps, improving the degree of separation, and avoiding cumbersome operations
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Embodiment 1
[0034] (1) Dilute the original Apt29-FAM stock solution with pure water to obtain 1.0×10 -6 mol / L Apt29-FAM sample solution; the original H-Thr stock solution was diluted with pure water to obtain 1.0×10 -6 mol / L H-Thr sample solution.
[0035] In the Beckman P / ACE MDQ capillary electrophoresis instrument, separate complexes based on two online reactions of capillary electrophoresis: take 20 μL of H-Thr sample solution, first-stage electrophoresis buffer, second-stage electrophoresis buffer and Apt29-FAM sample Solution, the order of sample injection during electrophoresis operation is: the first reactant H-Thr sample solution with slow migration rate, the first segment of electrophoresis buffer, the second reactant H-Thr sample solution with slow migration rate, the second segment Electrophoresis buffer and the third reactant Apt29-FAM sample solution with fast migration rate, the injection conditions are as follows: H-Thr sample solution: 0.5psi, 10s, the first electrophore...
Embodiment 2
[0040] (1) Dilute the original Apt29-FAM stock solution with pure water to obtain 1.0×10 -8 mol / L Apt29-FAM sample solution; the original H-Thr stock solution was diluted with pure water to obtain 1.0×10 -6 mol / L H-Thr sample solution.
[0041] In the Beckman P / ACE MDQ capillary electrophoresis instrument, two online reactions based on capillary electrophoresis were used to separate the complex: take 20 μL of H-Thr sample solution, the first segment of electrophoresis buffer, the second segment of electrophoresis buffer and Apt29-FAM Sample solution, the order of sample injection during electrophoresis operation is: the first reactant H-Thr sample solution with slow migration rate, the first electrophoresis buffer, the second reactant H-Thr sample solution with slow migration rate, the second Section electrophoresis buffer solution and the third reactant Apt29-FAM sample solution with fast migration rate; the sampling conditions are as follows: the fixed Apt 29-FAM sample sol...
Embodiment 3
[0047] (1) Dilute the original Apt29-FAM complementary sequence stock solution with pure water to obtain 1.0×10 -6mol / L Apt29-FAM complementary sequence sample solution; dilute the original H-Thr stock solution with pure water to obtain 1.0×10 -6 mol / L H-Thr sample solution.
[0048] In the Beckman P / ACE MDQ capillary electrophoresis instrument, two online reactions based on capillary electrophoresis were used to separate the complex: take 20 μL of H-Thr sample solution, the first segment of electrophoresis buffer, the second segment of electrophoresis buffer and Apt29-FAM Complementary sequence sample solution, the order of sample injection during electrophoresis is: the first reactant H-Thr sample solution with slow migration rate, the first electrophoresis buffer, the second reactant H-Thr sample solution with slow migration rate, The second section of electrophoresis buffer and the third reactant Apt29-FAM complementary sequence sample solution with fast migration rate; t...
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Abstract
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