Detection method of phenolphthalein in weight loss health food and preparation method of detection card
A technology of health food and detection method, applied in measuring devices, instruments, material analysis by optical means, etc., can solve the problems of high detection cost, missed detection, and high requirements for professional quality of personnel
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Embodiment 1
[0036] Embodiment 1: the rapid detection method of phenolphthalein of common health food that does not contain chlorophyll, tea polyphenol
[0037] see figure 1 , figure 2 , the detection process is as follows:
[0038] 1) Pretreatment of the sample to be tested: Weigh 0.5g of sample, put it into a 10ml centrifuge tube, add 3ml of extract, 0.1g of extract, shake for 2 minutes, let stand for 15 minutes, filter through a microporous membrane (0.45um) Filter, take the filtrate and add an equal volume of pure water to be the sample treatment solution;
[0039] 2) Qualitative detection: Add 60 microliters of the sample treatment liquid to the phenolphthalein detection card dropwise. If the test paper is pink, it is preliminarily proved that the sample to be tested contains phenolphthalein, which can be compared with the standard color spectrum for qualitative analysis;
[0040]3) Quantitative detection: 15 minutes after the qualitative detection is completed, the detection card...
Embodiment 2
[0041] Example 2: Rapid detection method for phenolphthalein in teas containing chlorophyll for weight loss and health food
[0042] see figure 1 , figure 2 , the detection process is as follows:
[0043] 1) Pretreatment of the sample to be tested: crush about 0.5 g of the sample, put it into a 10 ml centrifuge tube, add 3 ml of extract, 0.25 g of extract, shake for 2 minutes, let stand for 15 minutes, filter through a microporous membrane (0.45 um) to filter, take the filtrate and add an equal volume of pure water to be the treatment solution for the sample to be tested;
[0044] 2) Qualitative detection: Add 60 microliters of the sample treatment solution to the phenolphthalein test card dropwise. If the test paper is pink, it is preliminarily proved that the sample to be tested contains phenolphthalein, which can be compared with the standard color spectrum for qualitative analysis;
[0045] 3) Quantitative detection: 15 minutes after the qualitative detection is comple...
Embodiment 3
[0046] Example 3: Rapid detection method for phenolphthalein in tablets containing tea polyphenols for weight loss and health care
[0047] see figure 1 , figure 2 , the detection process is as follows:
[0048] 1) Pretreatment of the sample to be tested: take about 0.5 g of the sample, crush it, put it into a 10 ml centrifuge tube, add 3 ml of extract, 0.1 g of extract, 0.1 g of extract, shake for 2 minutes first, and shake every 10 minutes 1 minute, repeat 3 times, and finally stand still for 10 minutes, filter with a microporous membrane (0.45um), take the filtrate and add an equal volume of pure water to be the sample treatment solution;
[0049] 2) Qualitative detection: Add 60 microliters of the sample treatment solution to the phenolphthalein test card dropwise. If the test paper is pink, it is preliminarily proved that the sample to be tested contains phenolphthalein, which can be compared with the standard color spectrum for qualitative analysis;
[0050] 3) Quant...
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