Rapid detection method of nifedipine in blood pressure reducing health foods, and detection card developing method

A nifedipine and health food technology, which is applied in the field of nifedipine rapid detection method and detection card development, can solve the problems of only qualitative detection, high professional quality requirements of personnel, and high detection cost, and achieve the goal of simplifying the sample pretreatment process Effect

Active Publication Date: 2016-08-17
北京倍肯恒业科技发展股份有限公司
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Problems solved by technology

[0008] In order to solve the existing detection methods for whether antihypertensive health foods contain the chemical ingredient nifedipine, there are false detections and missed detections, only qualitative detections, high detection costs, long time-consuming, high requirements for professional quality of personnel and sample pretreatment Complicated and cumbersome problems, the present invention provides a rapid detection method for nifedipine in antihypertensive health food and a method for developing a detection card

Method used

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  • Rapid detection method of nifedipine in blood pressure reducing health foods, and detection card developing method
  • Rapid detection method of nifedipine in blood pressure reducing health foods, and detection card developing method
  • Rapid detection method of nifedipine in blood pressure reducing health foods, and detection card developing method

Examples

Experimental program
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Effect test

Embodiment 1

[0042] Example 1 : Rapid detection method for nifedipine in tea antihypertensive health drinks

[0043] see figure 2 , image 3 , the detection process is as follows:

[0044] 1) Pretreatment of samples to be tested: Accurately weigh 0.5g of the sample into a 5ml test tube, add 0.1g of extractant A and 1.5ml of extractant B, shake well for 2 minutes, let stand for 10 minutes, filter with 0.45um microporous After membrane filtration, take 0.25 ml of the filtrate and add 0.25 ml of the sample diluent C, which is the sample treatment solution to be tested;

[0045] 2) Qualitative detection: Add 70ul of the diluent dropwise to the nifedipine test paper card. If the test paper is orange-red, it is preliminarily proved that the sample to be tested contains nifedipine, which can be compared with the standard color spectrum for qualitative analysis;

[0046] 3) Quantitative detection: After 15 minutes of qualitative detection, place the test card in the intelligent comprehens...

Embodiment 2

[0047] Example 2 : Rapid detection method for nifedipine in capsule antihypertensive health food

[0048] see figure 2 , image 3 , the detection process is as follows:

[0049] 1) Pretreatment of samples to be tested: Take the contents of 1 capsule in a test tube, add 0.1g of extractant A and 1.5ml of extractant B, shake well for 2 minutes, let stand for 10 minutes, and filter with a 0.45um microporous membrane Finally, take 0.25 ml of filtrate and add 0.25 ml of sample diluent C, which is the sample treatment solution to be tested.

[0050] 2) Qualitative detection: Add 70ul of the diluent dropwise to the nifedipine test paper card. If the test paper is orange-red, it is preliminarily proved that the sample to be tested contains nifedipine, which can be compared with the standard color spectrum for qualitative analysis;

[0051] 3) Quantitative detection: After 15 minutes of qualitative detection, place the test card in the intelligent comprehensive detector for haz...

Embodiment 3

[0052] Example 3 : Nifedipine rapid detection card preparation method

[0053] see Figure 4 , the preparation process is as follows:

[0054] 1) Preparation of test paper substrate: use neutral filter paper as the substrate of nifedipine detection test paper;

[0055] 2) Preparation of chromogenic solution: Take 20ml of sodium hydroxide (1-10%) solution and 2ml of alkyl glycoside (APG0810) (5-20%) solution; put each component into the reactor and mix to prepare chromogenic solution 1; Take 10ml of sodium bicarbonate (1-10%) solution and 5ml of fatty alcohol ether sulfate (1-10%) solution; put each component into the reactor and mix to prepare color developing solution 2

[0056] 3) Soak the base material of the test paper: firstly, put the base material of the nifedipine detection test paper into the reagent chromogenic solution 1 and soak for 10 minutes; After drying for 20 minutes, let it cool; finally, put the nifedipine detection test paper substrate into the reag...

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Abstract

The invention discloses a rapid detection method of nifedipine in blood pressure reducing health foods, and a detection card developing method. The detection method comprises the following steps: 1, immersing a sample to be detected in an extract agent A and an extract liquid B, oscillating, and standing to extract nifedipine; 2, taking the obtained immersion solution, and filtering the immersion solution through a micro-porous filter membrane; 3, taking a certain amount of the obtained filtrate, and adding a sample dilution solution C according to a volume ratio of the sample dilution solution to the filtrate of 1:1 to obtain a sample-to-be-detected treatment solution; 4, taking 70[mu]L of the ample-to-be-detected treatment solution, adding the ample-to-be-detected treatment solution to a nifedipine detection card in a dropwise manner, observing the change of the color of test paper, preliminarily determining that the sample contains nifedipine if the test paper is yellow or orange red, and comparing the color with a standard color gradation spectrum to realize qualitative analysis; and 5, using a hand-held gold marking color developing instrument to carry out quantitative detection. The detection card developing method includes the following steps: preparing a test paper substrate, preparing a color developing solution, immersing the test paper substrate, preparing detection test paper, and preparing a detection card. The detection method is suitable for onsite rapid detection of the chemical component nifedipine in the blood pressure reducing health foods.

Description

technical field [0001] The invention belongs to the technical field of food safety detection, and in particular relates to a rapid detection method for nifedipine in antihypertensive health food and a method for developing a detection card. Background technique [0002] In recent years, with the improvement of people's economic conditions and health awareness, the market prospect of health food is bright. However, in pursuit of the so-called curative effect and huge profits, unscrupulous traders often illegally add chemical ingredients to health foods that are advertised as pure Chinese medicine preparations. [0003] At present, most of the antihypertensive health care products on the market are advertised as tea drinks or pure traditional Chinese medicine preparations. However, in order to achieve the purpose of lowering blood pressure in a short period of time, some products are artificially added with the calcium antagonist nifedipine. [0004] Nifedipine, the chemical ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78G01N21/17G01N1/28G01N1/38
CPCG01N1/28G01N1/38G01N21/17G01N21/78G01N2021/1734
Inventor 姚世平刘光中姚洪涛李心乐周轶
Owner 北京倍肯恒业科技发展股份有限公司
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