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Method for detecting quality of compound Cassia occidentalis tea

A quality inspection method, the technology of compound cassia tea, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of ineffective control of product quality, unfavorable human body, and poor specificity of the method, so as to ensure clinical efficacy and safety The effect of high specificity, simple measurement method and strong specificity

Inactive Publication Date: 2016-08-17
MUDANJIANG YOUBO PHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Compound cassia tea has the function of purging turbidity and moistening intestines, and is suitable for adjuvant treatment of hyperlipidemia due to qi deficiency and intestinal dryness. The item uses protocatechuic acid as a control, the method has poor specificity and cannot effectively control the quality of the product, and the developer contains benzene, which is harmful to the human body; the determination method of cassia seed content is complicated, with low accuracy and poor reproducibility

Method used

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  • Method for detecting quality of compound Cassia occidentalis tea
  • Method for detecting quality of compound Cassia occidentalis tea
  • Method for detecting quality of compound Cassia occidentalis tea

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] Identification of Danshen

[0054] 1 identification method

[0055] (1) Take the content of 1 bag of compound cassia tea, crush it, pass it through a No. 3 sieve, take 1g, add 20ml of water to dissolve, centrifuge, take the supernatant, adjust the pH value to 2 with dilute hydrochloric acid, add 20ml of ethyl acetate, Extract by shaking, recover the extract to dryness, add 5ml of methanol to the residue to dissolve, and use it as the test solution;

[0056] (2) Take the Danshensu sodium reference substance, add methanol to make a solution containing 0.1mg per 1ml, and use it as the reference substance solution; take 1g of the negative sample that does not contain Salvia miltiorrhiza, add 20ml of water to dissolve, centrifuge, take the supernatant, and use dilute Adjust the pH value to 2 with hydrochloric acid, add 20ml of ethyl acetate, shake and extract, recover the extract to dryness, add 5ml of methanol to the residue to dissolve, and use it as a negative test solutio...

Embodiment 2

[0074] Determination of Compound Cassia Tea

[0075] 1 Determination method

[0076] 1.1 Instruments, reagents and reagents

[0077] Instrument: Shimadzu LC-2010C HT High Performance Liquid Chromatograph: Shimadzu Corporation, Japan; Column: Diamonsil C18 150×4.6mm, 5μm; Aglient ZOBARX C18 250×4.6mm; Phenomenex luna C18 250×4.6mm; SCL-LC -10Avp ultraviolet-visible detector; electronic balance: CP225D, Beijing Sartorius Instrument System Co., Ltd.

[0078] Reference substances: orange cassiatin (provided by China Institute for the Control of Pharmaceutical and Biological Products, batch number: 111900-201202), chrysophanol (provided by China Institute for the Control of Pharmaceutical and Biological Products, batch number: 110796-200513).

[0079] Reagent: acetonitrile (chromatographically pure); the reagents used in the preparation of the test sample and reference substance are analytically pure.

[0080] Sample: compound cassia tea (batch numbers: 140302, 140304, 140311). ...

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Abstract

The invention discloses a method for detecting the quality of compound cassia tea. The method includes the step of identifying Danshen. The identification of Danshen is to identify Danshen by thin-layer chromatography, and Danshensu sodium is used as a reference substance. Chloroform: ethyl acetate: acetone: formic acid mixed solution with a volume ratio of 20:10:15:4 is used as a developing solvent; it also includes orange-yellow cassiatin and chrysophanol as reference substances, and the content of cassia seed is determined by high performance liquid chromatography. Carry out the assay step. The identification method of Salvia miltiorrhiza and the content determination method of Semen Cassiae of the present invention are simple, specific, high in accuracy and good in reproducibility. In the production process of the product, the quality of the product can be effectively controlled by the method of the present invention, thereby ensuring Clinical efficacy and safety of Mingcha.

Description

technical field [0001] The invention relates to a method for detecting the quality of compound cassia tea. It belongs to the field of medicine. Background technique [0002] With the rapid development of the economy, people's life rhythm is becoming more and more tense. Overtime, staying up late, and high pressure in life lead to irregular life, and common clinical diseases such as constipation appear. If things go on like this, toxins in the body cannot be discharged in time, which can induce tumors, etc. more serious illness. Compound cassia tea has the function of purging turbidity and moistening intestines, and is suitable for adjuvant treatment of hyperlipidemia due to qi deficiency and intestinal dryness. In this item, protocatechuic acid was used as the control, the specificity of the method was poor, and the quality of the product could not be effectively controlled, and the developing agent contained benzene, which was harmful to the human body; the determination ...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06G01N30/90G01N30/91G01N30/94
CPCG01N30/02G01N30/06G01N30/90G01N30/91G01N30/94G01N2030/027
Inventor 刘梅森黄靖梅笔雪艳金星宋莉萍高俊清张洪昌刘淑梅陈立红刘德峰周振东李佳一刘洋
Owner MUDANJIANG YOUBO PHARMA CO LTD
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