A method for testing the efficacy of duck Tembusu virus disease vaccine

A technique for duck tambusu virus and an inspection method is applied in the field of inspection of veterinary biological products to achieve the effect of an accurate method
CN105866424BActive Publication Date: 2017-05-24BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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Patent Information

Authority / Receiving Office
CN ยท China
Patent Type
Patents(China)
Current Assignee / Owner
BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
Publication Date
2017-05-24

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Abstract

The invention provides a serological testing method for the potency of an inactivated vaccine against duck Tembusu viral diseases. According to the method, HI antibody-negative ducks are used and divided into two groups, i.e., an immunized group consisting of ten ducks and a control group consisting of five ducks; each duck in the immunized group receives hypodermic or intramuscular injection of a vaccine against duck Tembusu viral diseases; immunization with an inactivated vaccine is carried out twice, a dosage of 0.5 ml or 1.0 ml per duck is used each time, and secondary immunization is carried out in two weeks after primary immunization; for immunization with a live vaccine, each duck is immunized according to a dosage of a standard vaccine usage amount for poultry once; in 3 to 4 weeks after inoculation, blood is acquired, serum is separated and the titer of an HI antibody is determined; and when the titer of the HI antibody in the ducks of the control group is less than 1: 5 and the titer of the HI antibody in serum of at least seven ducks of the immunized group is no less than 1: 10, it is determined that the potency of the vaccine is qualified in testing. The serological testing method provided by the invention is convenient to operate and accurate in results and can be extensively applied to potency evaluation of vaccines against duck Tembusu viral diseases and formulation of immune procedure in primary-level organizations and vaccine development and examination units.
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Description

technical field

[0001] The invention relates to a method for testing the efficacy of a veterinary vaccine, in particular to a method for testing the efficacy of a duck Tembusu virus disease vaccine, which belongs to the technical field of testing biological products for animals. Background technique

[0002] Duck Tembusu virus disease is a new or sudden infectious disease that occurred in 2010. Its causative agent is Duck Tembusu Virus (DTMUV), which belongs to the Flaviviridae family in terms of virus classification. The main clinical features are a sudden drop in the egg production of laying ducks and a significant increase in the death rate of ducks. The main gross lesions in cases of natural infection and artificial infection are follicle deformation, hemorrhage, liquefaction and spleen volume changes (spleen enlargement in the early stage of infection, splenomegaly in the later stage). zoom out) and the color turns black. The main histological lesions: reduction in the...

Claims

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