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Preparation of quercetin-3-o-α-l-arabinose-(1→2)-α-l-rhamnoside from jujube leaves and its application

A technology of arabinose and rhamnoside, which is applied in the extraction of quercetin-3-O-α-L-arabinose-α-L-rhamnoside, and the field of extracting and preparing active ingredients of plants, which can solve the problem of poor yield High, complex extraction and purification process, not flavonoid glycoside preparation process, etc., to achieve the effect of short preparation cycle, extensive practical value, and simple preparation process

Active Publication Date: 2021-03-12
TARIM UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are studies on the detection and identification of flavonoid glycosides in the leaves of C. chinensis, which involve the extraction and purification process, but mainly the steps required for the determination, not the preparation process of flavonoid glycosides, and the extraction and purification process is complicated, and the yield is low. high

Method used

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  • Preparation of quercetin-3-o-α-l-arabinose-(1→2)-α-l-rhamnoside from jujube leaves and its application
  • Preparation of quercetin-3-o-α-l-arabinose-(1→2)-α-l-rhamnoside from jujube leaves and its application
  • Preparation of quercetin-3-o-α-l-arabinose-(1→2)-α-l-rhamnoside from jujube leaves and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1: Extraction of quercetin-3-O-α-L-arabinose-(1→2)-α-L-rhamnoside from jujube leaves

[0032] The specific extraction method steps for preparing quercetin-3-O-α-L-arabinose-(1→2)-α-L-rhamnoside from jujube leaves are as follows:

[0033] (1) Pretreatment of raw materials: Harvest fresh red jujube leaves without insect erosion, wash them with clean water, dry them at 45°C, crush them, and pass them through a 40-60 mesh sieve.

[0034] (2) Extraction and concentration: the pulverized red jujube leaves prepared in step (1) were extracted with 75% ethanol solution, and the ratio of solid to liquid was 1:10, that is, g / ml. After 2 h, filter, combine the filtrates for 4 times, and concentrate the extract under reduced pressure at low temperature to obtain the total flavonoid extract with a yield of 13.0%.

[0035] (3) Elution: Dissolve the total flavonoids extract obtained in step (2) with 4 times the volume of distilled water, and then put it on a macroporous resin ...

Embodiment 2

[0043] Example 2: Preparation of quercetin-3-O-α-L-arabinose-(1→2)-α-L-rhamnoside from jujube leaves

[0044] The specific extraction method steps for preparing quercetin-3-O-α-L-arabinose-(1→2)-α-L-rhamnoside from jujube leaves are as follows:

[0045] (1) Pretreatment of raw materials: Harvest fresh red jujube leaves in November without insect erosion, wash with clean water, dry at 45°C, crush, crush and pass through a 40-mesh sieve.

[0046] (2) Extraction and concentration: Weigh 500 g of crushed jujube leaves prepared in step (1) and extract with 5 L of 75% ethanol solution, reflux extraction at 60°C for 2 h each time, filter, and combine for 4 times The filtrate and the extract were concentrated under reduced pressure at low temperature to obtain the total flavonoid extract with a yield of 13.0%.

[0047] (3) Elution: Dissolve the total flavonoid extract obtained in step (2) with 500 ml of water and apply it to a macroporous resin column at a flow rate of 1 ml / min. Afte...

Embodiment 3

[0054] Harvest red jujube leaves, wash them with clean water, dry them at 45°C, grind the dried red jujube leaves with a pulverizer, extract the dried sample powder with 75% ethanol solution, concentrate under reduced pressure at low temperature, and obtain the total flavonoid extract with a yield of 13.0% . The total flavonoid extract was dissolved in an appropriate amount of water and applied to a macroporous resin column, eluted with 20%, 50%, and 95% ethanol solutions respectively, collected 50% of the eluate, and recovered the solvent to obtain crude flavonoid glycosides with a yield of 3.5%. The crude flavonoid glycosides were dissolved in 3 times the volume of 50% methanol and refined and purified by preparative HPLC, the eluent was detected by analytical HPLC, the same fractions with a purity greater than 98% were combined, the solvent was recovered, and quercetin was obtained respectively Quercetin-3-O-bacoside, quercetin-3-O-rutinoside and a rare flavonoid glycoside ...

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Abstract

The invention discloses the preparation of quercetin-3-O-α-L-arabinose-(1→2)-α-L-rhamnoside from jujube leaves and the application thereof. The harvested jujube leaves are washed with clean water and dried. Dry, pulverize, extract with 75% ethanol solution, concentrate under reduced pressure at low temperature to obtain the total flavonoid extract, dissolve it with an appropriate amount of water, and put it on a macroporous resin column, elute with 20%, 50%, and 95% ethanol solution respectively, and collect 50% of the flavonoids. Remove the liquid, recover the solvent to obtain the crude flavonoid glycoside, dissolve it with 3 times the volume of 50% methanol, refine and purify it with a preparative high performance liquid phase, and use an analytical high performance liquid phase to detect the eluent, combine the fractions that are the same and have a purity greater than 98%, The flavonoid glycoside quercetin-3-O-α-L-arabinose-(1→2)-α-L-rhamnoside monomer is obtained by recovering the solvent. The product has high purity and high yield and can be used as a standard for analysis and detection The product has obvious application effect on the treatment of Leishmania and has extensive practical value.

Description

technical field [0001] The invention relates to the technical field of extraction and preparation of plant active ingredients. Specifically, the invention relates to the technical field of extracting quercetin-3-O-α-L-arabinose-(1→2)-α-L-rhamnoside for treating Leishmania. Background technique [0002] Jujube tree belongs to Rhamnaceae ( Rhamnaceae ) Jujube ( Ziziphus ) plant, the fruit of which is called jujube or jujube, which is not only a delicious fruit and dried fruit, but also a Chinese medicine with remarkable curative effect. At present, domestic and foreign processing and technical research are limited to jujube fruit, and there are few studies on other resources of jujube tree, such as roots, stems, leaves, flowers and bark. As of 2015, the planting area of ​​jujube trees in the country has reached 17 million mu. Jujube leaves are the largest by-product of jujube tree production. The annual output of fresh jujube leaves per mu of jujube fields is about 200 kil...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07H17/07C07H1/08
CPCC07H1/08C07H17/07
Inventor 白红进陈朋邹玲周忠波吴翠云
Owner TARIM UNIV
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