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A kind of alkaloid compound with antibacterial activity in tobacco, its preparation method and use

A technology of alkaloids and compounds, which is applied in the field of tobacco chemistry, can solve problems such as unidentified components, and achieve the effects of easy artificial synthesis, simple preparation and operation process, and good antibacterial activity

Active Publication Date: 2018-08-03
CHINA TOBACCO YUNNAN IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, more than 3,000 monomeric chemical substances have been identified from tobacco, and many components have not yet been identified

Method used

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  • A kind of alkaloid compound with antibacterial activity in tobacco, its preparation method and use
  • A kind of alkaloid compound with antibacterial activity in tobacco, its preparation method and use
  • A kind of alkaloid compound with antibacterial activity in tobacco, its preparation method and use

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Preparation of alkaloid compounds C 15 H 13 NO 3 , including extract extraction, silica gel column chromatography, high-pressure liquid chromatography separation, the specific steps are as follows:

[0028] 1. Extraction of extract: take tobacco leaves and pulverize, use high concentration methanol (w%: 95%) or high concentration ethanol (w%: 95%) or high concentration acetone (w%: 70%) as extraction solvent, extraction solvent: Tobacco (weight ratio)=3:5, soaked for 54 hours, extracted 4 times, combined with extracts, filtered and concentrated into extract.

[0029]2. Silica gel column chromatography: The extract is dissolved in pure methanol, pure ethanol or pure acetone with a weight ratio of 2.5 times, and then mixed with 80-100 mesh silica gel with a weight ratio of 1.2 times, and 250 mesh silica gel with a weight ratio of 3 times. Dry packing for silica gel column chromatography; chloroform in volume ratio (1:0, 20:1, 9:1, 8:2, 7:3, 6:4, 1:1 and 1:2) - Gradien...

Embodiment 2

[0034] Tobacco samples were from Yuxi, Yunnan, and the variety was Yuxi K326. 2.0kg of tobacco samples were pulverized and extracted with 95% methanol for 5 times, each extraction was 24h, the extracts were combined, filtered, and concentrated under reduced pressure to obtain an extract, 105g. The extract was dissolved in pure methanol with a weight ratio of 2.0 times, mixed with 120g of 100-mesh crude silica gel, and 0.6kg of 160-mesh silica gel was packed into a column for silica gel column chromatography. The volume ratio was 1:0, 20:1, 9:1, 8:2, 7:3, 6:4, 1:1, 1:2 chloroform-acetone gradient elution, TLC monitoring and merging the same fractions to obtain 8 fractions, wherein the volume ratio is 8: The chloroform-acetone eluted part of 2 was separated by Agilent 1100 semi-preparative high performance liquid chromatography, 52% methanol was used as the mobile phase, Zorbax SB-C18 (21.2×250mm, 5μm) preparative column was used as the stationary phase, and the flow rate was 20...

Embodiment 3

[0036] Tobacco samples are from Dali, Yunnan, the variety is Yunyan 200, 3.5kg of tobacco samples are chopped, extracted with 95% ethanol for 4 times, each extraction is 48h, the extracts are combined, filtered, and concentrated under reduced pressure to obtain an extract. 250g. The extract was dissolved in pure methanol with a weight ratio of 2.0 times, mixed with 250 g of 80-mesh thick silica gel, and 1.2 kg of 200-mesh silica gel was packed into a column for silica gel column chromatography. The volume ratio was 1:0, 20:1, 9:1, 8:2, 7:3, 6:4, 1:1, 1:2 chloroform-acetone gradient elution, TLC monitoring and merging the same fractions to obtain 8 fractions, wherein the volume ratio is 8: The chloroform-acetone eluted part of 2 was separated by Agilent 1100 semi-preparative high performance liquid chromatography, 52% methanol was used as the mobile phase, Zorbax SB-C18 (21.2×250mm, 5μm) preparative column was used as the stationary phase, and the flow rate was 20ml / min, the ...

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Abstract

The invention discloses an alkaloid compound. The alkaloid compound is chemically named as 8-hydroxy-3-hydroxymethyl-6,9-dimethyl-7H-benzo[de]isoquinoline-7-one and has a chemical formula C15H13NO3 and the structure shown in the specification. The invention further discloses a method for preparing the alkaloid compound from tobaccos. The invention further discloses use of the alkaloid compound. Activity tests prove that the alkaloid compound has a relatively good bacteriostatic action. The alkaloid compound is of a novel structure and has relatively good antibacterial activity. By applying the alkaloid compound to tipping paper of cigarettes, the possibilities of breeding and reproduction of bacteria in the tipping paper of the cigarettes can be eliminated or reduced.

Description

technical field [0001] The invention belongs to the technical field of tobacco chemistry, and in particular relates to an alkaloid compound firstly extracted from tobacco. Meanwhile, the present invention also relates to a preparation method of the compound and its application in antibacterial tipping paper. Background technique [0002] Tobacco is the most chemically complex plant in the world, and its secondary metabolites are very rich. According to reports by Dube and Green in 1982, more than 2,549 chemical components have been identified in tobacco. By 2008, Rodgman and perfetti reported that , the total number of compounds found in tobacco, tobacco substitutes, and cigarette smoke is about 8,700. At present, more than 3,000 monomeric chemical substances have been identified from tobacco, and many components have not yet been identified. In addition to being mainly used for cigarette smoking, tobacco can also extract a variety of valuable chemical components, from whi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D221/14A01N43/42A01P1/00A01P3/00D21H27/10
CPCA01N43/42C07D221/14D21H27/10
Inventor 叶灵李超秦云华熊文范多青芮晓东申钦鹏杨光宇缪明明
Owner CHINA TOBACCO YUNNAN IND
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