Preparation method of cannabis sativa seed meal polypeptide

A hemp seed and enzymatic hydrolysis technology, which is applied in the field of polypeptide preparation of hemp seed meal, can solve the problems that protein resources are not fully developed and utilized, and reduce the added value of products, so as to achieve increased added value, good color and good quality Effect

Active Publication Date: 2016-09-07
DAQING BRANCH OF HEILONGJIANG ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, hemp seeds are mostly used to extract oil, and the degreased seed meal is mainly used in animal feed, ...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] This embodiment prepares the hemp seed meal polypeptide according to the following steps:

[0021] (1) The degreased hemp seed meal is crushed through a 60-mesh sieve to obtain hemp seed meal powder, and the hemp seed meal powder and distilled water are mixed at a weight ratio of 1:5 to obtain a hemp seed meal mixture.

[0022] (2) Stir the hemp seed meal mixture on a magnetic stirrer for 10 min, and adjust the pH of the mixture to 5.0 with 1 mol / L hydrochloric acid.

[0023] (3) After adding papain to the hemp seed dregs mixed solution, place it in a constant temperature water bath oscillator to carry out the first step of enzymolysis to obtain the hemp seed dregs enzymatic hydrolysis solution, and control the amount of papain added as the substrate quality 0.4%, the enzymolysis temperature is 70°C, and the enzymolysis time is 1h.

[0024] (4) After the first step of enzymatic hydrolysis, raise the temperature of the enzymatic hydrolysis solution to 95°C, and inactiva...

Embodiment 2

[0030] This embodiment prepares the hemp seed meal polypeptide according to the following steps:

[0031] (1) The degreased hemp seed meal is crushed through a 60-mesh sieve to obtain the hemp seed meal powder, and the hemp seed meal powder is mixed with distilled water at a weight ratio of 1:10 to obtain the hemp seed meal mixture.

[0032] (2) Stir the hemp seed meal mixture on a magnetic stirrer for 10 min, and adjust the pH of the mixture to 4.0 with 1 mol / L hydrochloric acid.

[0033] (3) After adding papain to the hemp seed dregs mixed solution, place it in a constant temperature water bath oscillator to carry out the first step of enzymolysis to obtain the hemp seed dregs enzymatic hydrolysis solution, and control the amount of papain added as the substrate quality 0.5%, the enzymolysis temperature is 60°C, and the enzymolysis time is 1.5h.

[0034] (4) After the first step of enzymatic hydrolysis, raise the temperature of the enzymatic hydrolysis solution to 95°C, and...

Embodiment 3

[0040] This embodiment prepares the hemp seed meal polypeptide according to the following steps:

[0041] (1) The degreased hemp seed meal is crushed through a 60-mesh sieve to obtain hemp seed meal powder, and the hemp seed meal powder and distilled water are mixed at a weight ratio of 1:8 to obtain a hemp seed meal mixture.

[0042] (2) Stir the hemp seed meal mixture on a magnetic stirrer for 10 minutes, and adjust the pH of the mixture to 3.0 with 1 mol / L hydrochloric acid.

[0043] (3) After adding papain to the hemp seed dregs mixed solution, place it in a constant temperature water bath oscillator to carry out the first step of enzymolysis to obtain the hemp seed dregs enzymatic hydrolysis solution, and control the amount of papain added as the substrate quality 0.3%, the enzymatic hydrolysis temperature is 50°C, and the enzymatic hydrolysis time is 2h.

[0044] (4) After the first step of enzymatic hydrolysis, raise the temperature of the enzymatic hydrolysis solution...

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PUM

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Abstract

The invention discloses a preparation method of cannabis sativa seed meal polypeptide. The preparation method comprises the following steps: (1) mixing cannabis sativa seed meal with distilled water, so as to prepare cannabis sativa seed meal mixed liquid; (2) adjusting the pH value of the cannabis sativa seed meal mixed liquid to be 3-5; (3) adding papain into the cannabis sativa seed meal mixed liquid, and carrying out first-step enzymolysis, so as to prepare enzymatic hydrolysate; (4) after the first-step enzymolysis is finished, and heating, so as to inactivate papain; (5) after the inactivation, adjusting the pH value of the enzymatic hydrolysate to be 6-7, adding neutral protease, and carrying out second-step enzymolysis, so as to prepare enzymatic hydrolysate; (6) after the second-step enzymolysis is finished, heating to inactivate the neutral protease; and (7) carrying out centrifugation, filtering and vacuum low-temperature freeze drying on the inactivated enzymatic hydrolysate, so as to obtain the cannabis sativa seed meal polypeptide. By virtue of the enzymolysis on the cannabis sativa seed meal by virtue of a compound enzymic method, the utilization efficiency of enzyme and the yield and the quality of cannabis sativa polypeptide are greatly increased. The preparation method is of great significance in the fine and deep processing of cannabis sativa seed meal and the increase of the addition value of cannabis sativa seeds.

Description

technical field [0001] The invention belongs to the technical field of deep processing of agricultural products, and relates to a preparation method of hemp seed meal polypeptide. Background technique [0002] Hemp (Cannabis sativa L.), also known as hemp and marijuana, is an annual herb belonging to the family Cannabis genus. Hempseed has high nutritional value and is rich in amino acids, unsaturated fatty acids, mineral elements and biologically active ingredients. Its development prospects have attracted more and more attention. Many developed countries are vigorously developing hempseed food . [0003] The protein content of whole hemp seeds is generally between 20% and 25%, while the protein content of the shelled seeds exceeds 40%. Hemp seeds are an excellent source of protein and have high development and research value. Hemp protein contains 21 kinds of amino acids, including 8 kinds of amino acids needed by the human body. 65% of hemp protein is edestin, which h...

Claims

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Application Information

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IPC IPC(8): C12P21/06
Inventor 宋淑敏魏连会董燕刘宇峰高宇
Owner DAQING BRANCH OF HEILONGJIANG ACAD OF SCI
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