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Magnetic fluorescent double-model-state probe targeted to brain glioma and preparation method thereof

A glioma, dual-modality technology, applied to preparations, pharmaceutical formulations, general/multi-functional contrast agents for in vivo experiments, etc., can solve the problems of blurred anatomical background, low spatial resolution, and low sensitivity, etc. Achieve good biocompatibility, simple synthesis process, mild and controllable reaction conditions

Inactive Publication Date: 2016-09-21
SHANGHAI NAT ENG RES CENT FORNANOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, MRI has attracted widespread attention from scholars due to its high spatial resolution, tissue contrast, and non-ionizing radiation, and has been widely used in clinics. However, its sensitivity is not high, and it is often necessary to inject contrast agents to improve sensitivity.
Fluorescence imaging is non-invasive, non-invasive, highly sensitive, and real-time, but has limitations such as low spatial resolution, blurred anatomical background, and single function.

Method used

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  • Magnetic fluorescent double-model-state probe targeted to brain glioma and preparation method thereof

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Experimental program
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Effect test

Embodiment 1

[0029] 1. Highly biocompatible Zn 0.4 Fe 2.6 o 4 Preparation of Magnetic Nanoparticles

[0030] FeSO 4 ·(NH 4 ) 2 SO 4 ·6H 2 O and ZnSO 4 Dissolve in 20ml of water so that the precursor reaches 1.73×10 -3 mol Fe 2+ , 2.67×10 -4 mol Zn 2+ the goal of. Next, mix 10ml of oleic acid, 10ml of water and 1g of NaOH, and magnetically stir at room temperature until a uniform solution is obtained. Furthermore, the precursor Fe 2+ and Zn 2+ The solution was poured into this homogeneous solution, and after a few minutes of stirring, the mixed solution turned dark brown. Finally, the solution was transferred into a 50ml reaction kettle, sealed, and heated at 230 degrees for 15 hours. After the reaction, cool to room temperature. The product is deposited at the bottom of the kettle, and the nanoparticles are taken out by dissolving with cyclohexane. Then add ethanol into the cyclohexane containing the nanoparticles to precipitate the nanoparticles, and finally wash th...

Embodiment 2

[0040] 1. Highly biocompatible Zn 0.4 Fe 2.6 o 4 Preparation of Magnetic Nanoparticles

[0041] FeSO 4 ·(NH 4 ) 2 SO 4 ·6H 2 O and ZnSO 4 Dissolve in 20ml of water so that the precursor reaches 1.73×10 -3 mol Fe 2+ , 2.67×10 -4 mol Zn 2+ the goal of. Next, mix 10ml of oleic acid, 10ml of water and 1g of NaOH, and magnetically stir at room temperature until a uniform solution is obtained. Furthermore, the precursor Fe 2+ and Zn 2+ The solution was poured into this homogeneous solution, and after a few minutes of stirring, the mixed solution turned dark brown. Finally, the solution was transferred into a 50ml reaction kettle, sealed, and heated at 230 degrees for 15 hours. After the reaction, cool to room temperature. The product is deposited at the bottom of the kettle, and the nanoparticles are taken out by dissolving with cyclohexane. Then add ethanol into the cyclohexane containing the nanoparticles to precipitate the nanoparticles, and finally wash th...

Embodiment 3

[0051] 1. Highly biocompatible Zn 0.4 Fe 2.6 o 4 Preparation of Magnetic Nanoparticles

[0052] FeSO 4 ·(NH 4 ) 2 SO 4 ·6H 2 O and ZnSO 4 Dissolve in 20ml of water so that the precursor reaches 1.73×10 -3 mol Fe 2+ , 2.67×10 -4 mol Zn 2+ the goal of. Next, mix 10ml of oleic acid, 10ml of water and 1g of NaOH, and magnetically stir at room temperature until a uniform solution is obtained. Furthermore, the precursor Fe 2+ and Zn 2+ The solution was poured into this homogeneous solution, and after a few minutes of stirring, the mixed solution turned dark brown. Finally, the solution was transferred into a 50ml reaction kettle, sealed, and heated at 230 degrees for 15 hours. After the reaction, cool to room temperature. The product is deposited at the bottom of the kettle, and the nanoparticles are taken out by dissolving with cyclohexane. Then add ethanol into the cyclohexane containing the nanoparticles to precipitate the nanoparticles, and finally wash th...

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Abstract

The invention discloses a magnetic fluorescent double-model-state probe targeted to brain glioma and a preparation method thereof. Plenty of sulfydryl and carboxyl modified by nanometer particle surfaces are utilized, DTDP can be utilized in the first place to activate the sulfydryl, then mercaptoethylamine is utilized to conduct amination, then connection with carboxyl type fluorescein is conducted, finally RGD is bonded to the carboxyl opposite to the nanometer particle surfaces; or, after EDC / NHS activates the carboxyl on the surfaces of the nanometer particle surfaces, RGD and carboxyl type fluorescein are directly connected to the nanometer particle surfaces. By means of the two methods, a targeting MRI / fluorescent double-model-state probe material can be obtained. The prepared molecular imaging probe achieves MR / fluorescent double-model-state imaging on the cell level and the anima level due to mediation of RGD.

Description

technical field [0001] The invention belongs to the field of preparation of dual-mode molecular imaging probes, in particular to a preparation method of a brain glioma-specific molecular imaging probe with nuclear magnetic / optical dual-mode imaging functions. Background technique [0002] Glioma is the most common malignant tumor of the central nervous system, with a high rate of death and disability, so it is one of the biggest problems in the field of tumor treatment. Early diagnosis and treatment are the key to cure cancer. In terms of early diagnosis of tumors, traditional imaging techniques can only understand tumor volume and anatomical location, while molecular imaging techniques allow us to obtain more valuable information, such as protein molecular detection before and after malignant transformation, and assessment of tumor growth kinetics , tumor cell markers, etc., and in vivo molecular imaging can realize the study of pathogenesis without damaging the microenvir...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K49/00A61K49/18A61K49/14
CPCA61K49/0002A61K49/0043A61K49/0056A61K49/0093A61K49/14A61K49/1833
Inventor 何丹农祝闪闪王萍金彩虹
Owner SHANGHAI NAT ENG RES CENT FORNANOTECH
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