Complex fungicide and preparing and application thereof

A compound bacterial agent and bacillus technology, applied in the application, fungicide, bacteria and other directions, can solve the problem of multi-strain mixed compound use without relevant literature reports, and achieve the effects of improving the quality of agricultural products, reducing costs, and increasing microbial flora.

Pending Publication Date: 2016-09-21
北京康缘益生生物科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

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Method used

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  • Complex fungicide and preparing and application thereof
  • Complex fungicide and preparing and application thereof
  • Complex fungicide and preparing and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Obtaining of strains:

[0050] 1. Collection of soil samples

[0051] (1) In summer, after rain. Collect soil samples within a range of about 5 cm to 10 cm below the surface of the crop rhizosphere soil;

[0052] (2) Select the diseased area, but the rhizosphere soil under the individual plants that have not diseased;

[0053] (3) Store in a sterile, air-permeable plastic bag.

[0054] 2. Enrichment culture

[0055] (1) Medium preparation

[0056] Ordinary nutrition agar plate g / L: beef extract 3.0, peptone 10.0, sodium chloride 5.0, purified water 1000ml, agar powder 2.0, pH=7.0~7.2, nystatin 50mg / L

[0057] (2) Soil sample treatment

[0058] Weigh 1g of soil sample, add it to 99ml of sterile water, shake for 15min with a shaker. Filter under sterile conditions to obtain filtrate

[0059] (3) Dilute the above-mentioned filtrate with sterile water according to the ten-fold gradient dilution method to 10 -3 , 10 -4 , 10 -5 , 10 -6

[0060] (4) Incubate the above diluent in a water bat...

Embodiment 2

[0092] Preparation of compound bacteria:

[0093] 1. The cultivation process of three strains of Bacillus amyloliquefaciens includes the following steps

[0094] 1) Preparation of solid slant strain

[0095] The three preserved strains were respectively inoculated on a slant medium, and kept standing at 37°C for 24 hours. The microscopic examination was free of bacteria and the spore rate was greater than 95%, and stored in a refrigerator at 4°C. Use within one year.

[0096] Used slope formula (weight ratio): glucose 0.1%, yeast extract powder 0.5%, peptone 0.1%, beef extract 0.5%, sodium chloride 0.02%, calcium chloride 0.01%, disodium hydrogen phosphate 0.04%, soluble starch 1.4%, ammonium sulfate 0.01%, manganese sulfate 0.1 mg / L, sodium molybdate 0.1 mg / L, agar powder 1.5%, and the balance is water. pH=7.1. 121℃, sterilize for 30min.

[0097] 2) Preparation of bottled liquid strain

[0098] Inoculate the fresh slant cultures of the three excellent strains obtained above into the ...

Embodiment 3

[0118] The difference from Example 2 is

[0119] 1. Preparation of solid slope strain

[0120] The preserved strains were inoculated on a slant medium, and incubated at 37°C for 24 hours. The microscopic examination showed no bacteria and the spore rate was greater than 95%, and stored in a refrigerator at 4°C. Use within one year.

[0121] The used slope formula (weight ratio): glucose 0.1%, yeast extract 0.5%, peptone 0.3%, beef extract 0.3%, sodium chloride 0.2%, calcium chloride 0.01%, disodium hydrogen phosphate 0.02%, soluble starch 2.0%, ammonium sulfate 0.01%, manganese sulfate 0.4mg / L, sodium molybdate 0.2mg / L, agar powder 2.0%, the balance is water. pH=7.1. 121℃, sterilize for 30min.

[0122] 2. Preparation of bottled liquid strain

[0123] The preserved fine slant strains are inoculated into liquid flask culture medium and cultured at 180rpm, 30℃~32℃, constant temperature shaking for 23h to obtain the flask liquid strains.

[0124] Liquid bottle formula used (weight ratio):...

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Abstract

The invention relates to a microorganism fungicide, in particular to a complex fungicide and preparing and application thereof. Bacillus amyloliquefaciens includes CGMCCNO.12306(KY-1), CGMCCNO.12307(KY-2) and CGMCCNO.12308(KY-3) respectively, and the CGMCCNO.12306(KY-1), the CGMCCNO.12307(KY-2) and the CGMCCNO.12308(KY-3) are collected in China General Microbiological Culture Collection Center. The bacillus amyloliquefaciens is subjected to three-grade liquid fermentation respectively, and then the fermentation liquid is mixed according to the volume ratio of the CGMCCNO.12306(KY-1) to the CGMCCNO.12307(KY-2) to the CGMCCNO.12308(KY-3) of (5-1):(1-5):(5-1) to obtain the complex fungicide. According to the microorganism fungicide, the bacillus amyloliquefaciens KY-1, the bacillus amyloliquefaciens KY-2 and the bacillus amyloliquefaciens KY-3 which are obtained according to rhizosphere soil of different crops in different territories have different physiological functions, and antagonism is not generated between the bacillus amyloliquefaciens KY-1, the bacillus amyloliquefaciens KY-2 and the bacillus amyloliquefaciens KY-3.

Description

Technical field [0001] The invention relates to a microbial inoculum, in particular to a composite inoculum and its preparation and application. Background technique [0002] Soil-borne diseases refer to diseases caused by pathogens living in the soil invading crops from the roots or stem bases of crops when conditions are suitable. The common ones are damping-off, blight, fusarium wilt, verticillium wilt, soft rot, root rot, blight, black rot, sclerotium, root-knot nematode and so on. This kind of disease is increasing year by year. The direct economic losses caused to agricultural production each year amount to 40%-60%, and the serious ones are more than 80%. Seriously restrict the development of facility agriculture. [0003] At present, the prevention and control methods of this disease mainly include crop rotation, breeding of disease-resistant varieties and the use of chemicals. Among them, crop rotation is widely used for disease prevention, but it cannot meet the requir...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01N63/02A01P5/00A01P7/04A01P1/00A01P3/00A01P21/00C05F11/08C12R1/07
CPCA01N63/00A01N63/10C05F11/08C12N1/20C12N1/205C12R2001/07
Inventor 张传军张晓红张中亲白玉红李冰洁
Owner 北京康缘益生生物科技有限公司
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