Method for determining content of momordin Ic in Fuyang granule (granule for itchy skin)

Abstract

The invention provides a method for determining the content of momordin Ic in a Fuyang granule. The objective of the invention is to provide the method with the advantages of more convenient operation, high sensitivity and good stability and reproducibility. The method comprises the following steps: weighing a sample of the Fuyang granule, carrying out detection on a reference solution and a test solution and calculating the content of momordin Ic, wherein chromatographic conditions are that a chromatographic column is Agilent SB-C18, 250 mm * 4.6 mm, 5 [mu]m; column temperature is 40 DEG C; a mobile phase is an acetonitrile-1% glacial acetic acid solution in a ratio of 90: 10; flow velocity is 0.8 ml/min; wavelength is 287 nm; and an ELSD detector has an atomizer temperature of 60 DEG C, a drift tube temperature of 80 DEG C and carrying gas N2 flow velocity of 1.0 L/min. The method belongs to the technical field of chemical detection.

Description

technical field [0001] The invention provides a method for the content of saponin Ic, specifically, a method for measuring the content of Kochia saponin Ic in Fuyang granules; it belongs to the technical field of chemical detection. Background technique [0002] Skin itching granule, dispels wind and activates blood circulation, dehumidifies and relieves itching. For pruritus, urticaria. Xanthium (stir-fried, thorn-free), Kochia scoparia, Chuanxiong, safflower, Baiying. The excipients are dextrin and stevia. [0003] The detection method given by the Chinese Pharmacopoeia is: take 4.0g of this product, grind it finely, add 40mL of ethanol, ultrasonically treat for 5min, filter, add 3.0mL of hydrochloric acid to the filtrate, heat and reflux for 2h, put the solution to room temperature, filter, and the filtrate in Ethanol was evaporated on a water bath to dryness, the residue was dissolved by adding 20 mL of water, transferred to a separatory funnel, extracted twice with p...

AI Technical Summary

Problems solved by technology

However, the operation process of the method is complicated, the...