Method for manufacturing tissue-engineered vascularized liver lobules

A manufacturing method and tissue engineering technology, applied in the field of biomedicine, can solve problems such as poor precision, time-consuming and labor-intensive, structures that cannot be processed, and achieve good biocompatibility

Active Publication Date: 2017-07-28
SHENZHEN GRADUATE SCHOOL TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, biological mold processing generally adopts technical means such as injection molding, machine tool turning, milling, planing and grinding, and the processing accuracy is poor, and it is time-consuming and laborious.
It cannot be processed for structures with special structural requirements

Method used

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  • Method for manufacturing tissue-engineered vascularized liver lobules
  • Method for manufacturing tissue-engineered vascularized liver lobules

Examples

Experimental program
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Embodiment Construction

[0017] The present invention will be further described below in conjunction with specific embodiments.

[0018] The manufacturing method of the tissue engineered vascularized liver lobules in this embodiment comprises the following steps:

[0019] Step 1, print the supportive PDMS frame of the liver lobules and make the mold of the liver lobules. The specific process is as follows:

[0020] The supporting frame is printed using polydimethylsiloxane (PDMS). Mix PDMS evenly according to the mass ratio of substrate and cross-linking agent at 10:1, add it into the syringe of the 3D printer, and print out the PDMS frame according to the set path. Insert seven capillary glass tubes into the PDMS frame, which constitutes the forming mold of the liver lobule, such as figure 1 shown. It is used after being sterilized by high temperature and high pressure.

[0021] Step 2: Mix the HepG2 cells with the temperature-sensitive hydrogel solution according to the cell concentration of 10...

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Abstract

The invention provides a manufacturing method of vascularized hepatic lobule for tissue engineering. The manufacturing method comprises the following steps: printing a compatible supportive frame of the hepatic lobule by adopting a 3D printer; inserting seven capillary glass tubes into the frame to form a forming mould of the hepatic lobule; uniformly mixing HepG2 cells and a thermo-sensitive hydrogel solution according to the cell concentration being 10<6> cells / mL, adding the obtained solution into the mould, carrying out irradiation for 30-200 s by adopting ultraviolet light, carrying out collagen cure-crosslinking, and drawing out the capillary glass tubes, thus forming a capillary channel; preparing human umbilical vein endothelial cells with the concentration being 1x10<7> cells / mL, injecting the human umbilical vein endothelial cells into the capillary channel, and carrying out culture, so that the human umbilical vein endothelial cells grow all over the inner wall of the capillary channel, and thus the vascularized hepatic lobule is obtained. The hepatic lobule prepared by adopting the method is good in biocompatibility, and can be used for the screening of liver drugs, the study on disease mechanisms, and the study on the metabolic process of the drugs in the liver.

Description

technical field [0001] The invention belongs to the field of biomedicine, and in particular relates to a method for manufacturing tissue-engineered vascularized liver lobules. Background technique [0002] 3D printing technology is also known as additive manufacturing. Its processing process is simple, and it does not need original embryos and models. It can directly use computer modeling to print out the desired structure. Compared with traditional processing methods, 3D printing is fast and accurate. , Easy processing characteristics. At present, bio-mold processing generally adopts technical means such as injection molding, machine tool turning, milling, planing and grinding, and the processing accuracy is poor, and it is time-consuming and laborious. It cannot be processed for structures with special structural requirements. [0003] Liver failure is a common killer of humans. The common treatment method is artificial liver. Artificial liver refers to removing various ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61F2/02
CPCA61F2/022
Inventor 李勃朱朋飞许国军王进周济
Owner SHENZHEN GRADUATE SCHOOL TSINGHUA UNIV
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