60Co gamma ray-induced mutation method for Capsule Epidendrum secundum
A gamma ray and tree orchid technology, which is applied in the field of 60Co-γ-ray radiation mutagenesis of tree orchid capsules, can solve problems that have not yet appeared, and the application has not been reported, and achieve the effects of convenient material collection, reduced pollution rate, and high germination rate
Inactive Publication Date: 2016-10-12
INST OF FORESTRY CHINESE ACAD OF FORESTRY
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Problems solved by technology
Radiation mutation breeding has been applied in the breeding of orchids in my country, but the application in Orchids has not been reported yet
And in the radiation mutagenesis of Orchidaceae, most scholars choose rhizomes, protocorms, complete plants of test-tube seedlings or rootless seedlings as radiation materials, and there has been no research on the use of complete capsules for 60Co-γ-ray radiation treatment
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[0077] 1. Materials:
[0078] Tree orchid (Epidendrum secundum) seeds are taken from the greenhouse of the Chinese Academy of Forestry, a species of tree orchid, produced in Brazil;
[0079] 2. Method:
[0080] (1) Medium and culture conditions
[0081] Medium for embryo germination experiment: MS, 1 / 2MS, 1 / 4MS.
[0082] Seed germination medium: 1 / 2MS
[0083] Protocorm differentiation medium: MS
[0084] Protocorm propagation medium: Huabao No. 1 improved medium
[0085] The pH of the above medium is 5.8-6.0, and the temperature is sterilized at 121° C. for 20 minutes. The culture conditions are temperature of 24(±2)°C, light intensity of 1600-2000lx, and light time of 14h / d.
[0086] (2) Radiation treatment
[0087] Radiation treatment was performed at the Beijing Radiation Center. The orchid capsules on the same plant with the same flowering period, pollination time, and growth vigor were taken as test materials, and the groups were irradiated with 60Co-γ rays. The ...
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The invention discloses a 60Co gamma ray-induced mutation method for Capsule Epidendrum secundum. The method comprises the following steps: selecting seeds of Epidendrum secundum, determining mediums and culture conditions, dividing the seeds into different groups, subjecting the groups to radiation by 60Co gamma rays, determining a half lethal dose LD50 through statistical analysis and carrying out fitting with related software so as to obtain a linear equation; inoculating differentiation mediums with protocorms germinated from the seeds, carrying out culture and differentiation so as to obtain regenerated plants, investigating the differentiation conditions and morphological indexes of the regenerated plants in 120 d, and analyzing difference between radiation groups receiving different dosages of radiation and a control group; and carrying out mutagenization with 60Co gamma rays under tissue culture conditions, observing mutagenization effect of different dosages of radiation on the seeds of Epidendrum secundum and determining a proper radiation dosage. According to the invention, 60Co gamma ray-induced mutation breeding technology for Capsule Epidendrum secundum is established, provides basis for selection and determination of the proper radiation dosage for radiation-induced mutation breeding of Capsule Epidendrum secundum and enables a novel variety to be bred. The method provided by the invention uses easily available raw materials, can reduce a contamination rate and saves time and cost.
Description
technical field [0001] The invention relates to a radiation mutagenesis technique for orchid capsules, in particular to a method for 60Co-γ-ray radiation mutagenesis of orchid capsules. Background technique [0002] Radiation mutagenesis is also known as physical mutagenesis. The use of radiation mutagenesis technology for breeding is a commonly used and highly efficient technology in the field of breeding. It mainly refers to treating materials with a certain dose of ray radiation, thereby inducing mutations in materials and causing mutations. (gene mutation or chromosomal aberration), and then through screening, determination, and selection, the breeding process in which new varieties that are useful for scientific research or production are finally selected from the mutants [1]. At present, in radiation mutagenesis research, commonly used radiation sources include: gamma and X-rays, neutrons, electron beams, ion beams, etc., in addition to non-ionizing radiation (ultravio...
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IPC IPC(8): A01H1/08A01H4/00
CPCA01H1/08A01H4/001A01H4/005A01H4/008
Inventor 李潞滨刘蕾周雅倩
Owner INST OF FORESTRY CHINESE ACAD OF FORESTRY