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Thin-layer identification method for Blumea riparia in Yixuean granules

A technology of thin-layer identification and Yixuan, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of difficult qualitative analysis, poor separation and reproducibility, etc., to achieve rapid qualitative analysis, repeat The effect of high visibility and quality control

Inactive Publication Date: 2016-10-12
广西万寿堂药业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the existing quality standards for Yixuan granules, although there is an analytical method for Ainaxiang Diangui, there are problems of poor resolution and reproducibility, and it is difficult to carry out the analysis of Ainaxiang Diangui effectively, accurately and quickly. qualitative analysis

Method used

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  • Thin-layer identification method for Blumea riparia in Yixuean granules
  • Thin-layer identification method for Blumea riparia in Yixuean granules

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Preparation of the test solution: take Yixuan granules with batch numbers of 120303, 120602, 120603, 120701, and 120702 for thin-layer chromatography identification. Each batch of Yixuean granules takes 5 g, respectively, and adds 30 mL of water to make Yixuan granules. Dissolve the granules, add 0.25 mL of dilute hydrochloric acid, stir evenly, filter, add ether to extract 3 times, 15 mL each time, combine the ether extracts, add water to wash 2 times, 15 mL each time, discard the water, and separate the ether extracts , Evaporate to dryness, add methanol 1mL to dissolve the residue.

[0024] Protocatechuic acid reference substance solution: add methanol to the protocatechuic acid reference substance to make a 0.30mg·mL-1 solution.

[0025] Protocatechualdehyde reference substance solution: add methanol to the protocatechualdehyde reference substance to make a 0.30mg·mL-1 solution.

[0026] Negative control solution: take the negative sample of Yixuean granules lackin...

Embodiment 2

[0029] Preparation of the test solution: take the Yixuean granules with batch numbers 120303, 120602, 120603, 120701, and 120702 respectively for thin-layer chromatography identification. Each batch of Yixuean granules takes 6g and adds 30mL of water to make Yixuean Dissolve the particles, add 0.3mL of dilute hydrochloric acid, stir evenly, filter, add diethyl ether to extract twice, 20mL each time, combine the diethyl ether extract, add water to wash twice, each 20mL, discard the water, and separate the diethyl ether extract, Evaporate to dryness, add methanol 2mL to the residue to dissolve it.

[0030] Protocatechuic acid reference substance solution: add methanol to the protocatechuic acid reference substance to make a 0.27mg·mL-1 solution.

[0031] Protocatechualdehyde reference substance solution: add methanol to the protocatechualdehyde reference substance to make a 0.32mg·mL-1 solution.

[0032] Negative control solution: take the negative sample of Yixuean granules la...

Embodiment 3

[0035] Preparation of the test solution: take Yixuan granules with batch numbers of 120303, 120602, 120603, 120701, and 120702 for thin-layer chromatography identification. Each batch of Yixuean granules takes 5 g, respectively, and adds 30 mL of water to make Yixuan granules. Dissolve the particles, add 0.25mL of dilute hydrochloric acid, stir evenly, filter, add diethyl ether to extract 3 times, 15mL each time, combine the diethyl ether extract, add water to wash 2 times, each 15mL, discard the water, and separate the diethyl ether extract , Evaporate to dryness, add methanol 1mL to dissolve the residue.

[0036] Protocatechuic acid reference substance solution: add methanol to the protocatechuic acid reference substance to make a 0.30mg·mL-1 solution.

[0037] Protocatechualdehyde reference substance solution: add methanol to the protocatechualdehyde reference substance to make a 0.30mg·mL-1 solution.

[0038] Negative control solution: take the negative sample of Yixuean ...

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Abstract

The invention discloses a thin-layer identification method for Blumea riparia in Yixuean granules. With protocatechuic acid and protocatechualdehyde as the contrast, and a chloroform, ethyl acetate and formic acid mixed solution as a developing solvent, the method is good in separation effect, high in reproducibility and easy to implement; by means of the method, rapid qualitative analysis of Blumea riparia can be achieved, and a reliable guarantee is provided for effectively controlling the quality of Yixuean granules.

Description

technical field [0001] The invention relates to the field of drug detection, in particular to a method for thin-layer identification of Diangui Ainaxiang in Yixue'an granules. Background technique [0002] Yixue'an Granules is a Chinese patent medicine for treating gynecological anemia. Clinically, it is used for persistent postpartum lochia, unclean uterine bleeding after artificial abortion, and blood stasis syndrome in TCM. [0003] The monarch drug of Yixuean Granules is Ainaxiang Diangui, which is derived from the dried whole herb of Ainaxiang [Blumea riparia (BL) DC.] of Compositae. It is mainly produced in southwestern Guangxi and southeastern Yunnan. Born in forest edges, hillside thickets or dense forests. In the existing quality standards for Yixuan granules, although there is an analytical method for Ainaxiang Diangui, there are problems of poor resolution and reproducibility, and it is difficult to carry out the analysis of Ainaxiang Diangui effectively, accura...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/90
CPCG01N30/90
Inventor 毛金玲慕丽群李修善农常东
Owner 广西万寿堂药业有限公司
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