A detection method for residual pesticides in closed environment of agricultural product logistics

A closed environment and detection method technology, applied in the direction of biological testing, measuring devices, material inspection products, etc., to achieve the effect of simple cultivation, simple operation, and easy acquisition

Active Publication Date: 2020-11-17
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0012] However, how to quickly and efficiently qualitatively detect and analyze pesticide residues in the air based on biological methods has not been reported.

Method used

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  • A detection method for residual pesticides in closed environment of agricultural product logistics
  • A detection method for residual pesticides in closed environment of agricultural product logistics
  • A detection method for residual pesticides in closed environment of agricultural product logistics

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Embodiment 1 of the present invention provides a method for detecting residual pesticides in a closed environment of agricultural product logistics, such as figure 1 As shown, it specifically includes the following steps:

[0049] S1. Incubation of brine shrimp eggs: Weigh 1g brine shrimp eggs (shrimp eggs are kept in a refrigerator at 4°C, take out the refrigerator before incubation, and return to room temperature, about 2-3h), place them in a gas-filled incubator, and add In 250ml of homemade seawater, incubate with aeration for 24-30h under light conditions; in order to reduce the diversity of organisms and ensure the uniformity of the test, generally use brine shrimp larvae with an incubation time of 26-28 hours as the test object.

[0050] S2. The poison test of brine shrimp: use common pesticides on the market as poison reagents, such as phoxim, profenofos, beta-cypermethrin, beta-deltamethrin, beta-half-cyhalothrin, acetamiprid, Thiamethoxam, imidacloprid, and chlorfe...

Embodiment 2

[0074] Embodiment 2 of the present invention provides a rapid qualitative detection method for residual pesticides in a closed environment of agricultural product logistics, which specifically includes the following steps:

[0075] S1. Incubation of brine shrimp eggs, weigh 1g brine shrimp eggs (shrimp eggs are kept in a refrigerator at 4℃, take out the refrigerator before incubation, return to room temperature, about 2-3h), put it in the aerated hatching device, add Incubate in 250ml of homemade seawater for 26h with aeration under light conditions.

[0076] S2. Place the adsorbent in the adsorption filtering device.

[0077] S3. Seal the device containing the adsorption material in an environment;

[0078] S4. After circulating filtration for 30 minutes in the ambient air of fruit and vegetable storage and transportation; then, the adsorbent is eluted, and 200 μL of the eluate is added to a 24-well plate, and then the hatched brine shrimp culture solution in step 1 is added 50μL;

...

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Abstract

The invention discloses a method for detecting residual insecticide in an agricultural product logistics closed environment. The method comprises the following steps: placing a device loaded with a broad-spectrum insecticide adsorption material in an agricultural product logistics closed environment, performing air circulation adsorption according to a preset mode, performing desorption treatmenton an insecticide on the adsorption material by using eluent to obtain desorption liquid, and preliminarily analyzing the residual quantity of the insecticide in the desorption liquid by using a tested living organism. The method provided by the invention can be used for quickly and qualitatively analyzing the insecticide residues in the agricultural product logistics closed environment; the adsorbing material is easy to obtain and low in cost, and has economic and operation feasibility; the biological living fairy shrimps are wide in source, easy to obtain and easy to culture, the time required for qualitatively detecting the pesticide residues in the air through the method is short, operation is easy, large instruments are not needed, the cost is low, and real-time and rapid qualitativedetection can be conducted in the fruit and vegetable logistics process.

Description

Technical field [0001] The invention relates to the technical field of residual pesticide detection, in particular to a method for detecting residual pesticides in a closed environment of agricultural product logistics. Background technique [0002] Artemia sp., also known as saltwater brine shrimp, fairy shrimp, and brine shrimp, belong to the arthropod phylum, with branchial subdivisions, crustaceans, branchial subclasses, and Amaranthes, saltwater brine shrimps; it is in Widely distributed worldwide, it is a small crustacean that can live in high-salt waters. It can be found in Europe, Central Asia and Western North America, many arid regions in Africa, and freshwater ponds in Australia. . The larvae of brine shrimp live by filtering and feeding organic matter in seawater and are sensitive to toxic substances. Ingestion of substances with cytotoxic, anti-cancer, antibacterial, and insecticidal activities can cause their death. [0003] With the rapid increase in the world's po...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/50
CPCG01N33/5085G01N2430/10
Inventor 姜微波徐延
Owner CHINA AGRI UNIV
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