A method for producing intestinal membrane protein powder by using enzymatic hydrolysis method for heparin to absorb residual liquid

A technology of intestinal membrane protein powder and enzymatic hydrolysis, which is applied in the direction of protein food components, protein components of waste, applications, etc., can solve problems such as poor efficacy, low protein recovery rate, and inability to exert the efficacy of intestinal membrane protein powder, and achieve good results. Social and economic benefits, increased additional income, and good feeding effects

Active Publication Date: 2019-06-07
JIANGNAN UNIV
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

From the analysis of the process description, the heparin residual liquid involved in this process should be the waste liquid after the heparin was extracted by the salting out method. In the salting out method, more than 80% of the intestinal membrane protein is in the intestinal membrane residue, and only less than 20% of the intestinal membrane protein is in the intestinal membrane residue. Membrane proteins are dispersed in the heparin raffinate, so the extraction consumes a lot of energy; on the other hand, practice shows that in the process of nanofiltration desalination and dehydration, small molecular weight small peptides and amino acids in the heparin raffinate will be lost with the brine through the nanofiltration membrane. That is to say, the recovery rate of the large molecular weight protein in the heparin raffinate is low by this method, and the efficiency of feeding is poor at the same time.
It is also reported that the filtrate obtained by resin adsorption and filtration is concentrated and dried to obtain intestinal membrane protein powder, but the intestinal membrane protein powder produced contains a large amount of salt (more than 50%), which can be mixed in the cub feed in the early weaning period. The proportion of intestinal membrane protein powder imported is extremely low, and the effect of intestinal membrane protein powder cannot be exerted, resulting in no practical use value of the protein powder

Method used

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  • A method for producing intestinal membrane protein powder by using enzymatic hydrolysis method for heparin to absorb residual liquid
  • A method for producing intestinal membrane protein powder by using enzymatic hydrolysis method for heparin to absorb residual liquid

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Embodiment 1

[0032] The technological process of the method for producing intestinal membrane protein powder by using enzymatic hydrolysis method heparin to absorb raffinate of the present invention is as follows figure 1 As shown, after the adsorption residual liquid is filtered through the membrane, the clear liquid and concentrated liquid are obtained. The clear liquid is evaporated, concentrated, and crystallized to separate and remove NaCl, and the remaining desalted mother liquid is mixed with the concentrated liquid of membrane filtration, and then dried to form intestinal membrane after preparation of auxiliary materials. Finished protein powder.

[0033] A sausage casing factory enzymatically hydrolyzed heparin sodium to absorb the residual liquid, with a total solid content of 4.95%, organic matter 1.97%, NaCl 2.50%, and a gray-white suspension.

[0034] Use 0.45μm microfiltration membrane to filter the heparin adsorption residual liquid in a cross-flow cycle. When the concentrat...

Embodiment 2

[0036] A sausage casing factory enzymatically hydrolyzed heparin sodium to absorb the residual liquid, with a total solid content of 4.42%, organic matter 1.28%, NaCl 2.77%, and a gray-white suspension.

[0037] Use the ultrafiltration membrane with a molecular weight cut off of 100,000 to filter the heparin adsorption residual liquid in a cross-flow cycle. When the concentration of solids in the concentrated liquid reaches 16%, the filtration is stopped to obtain a gray concentrated liquid and a transparent light yellow clear liquid; the clear liquid is first passed through four effects Evaporate and concentrate 10 times. At this time, salt particles have been precipitated. Transfer to a single-effect evaporator and continue to slowly concentrate 2.2 times at a temperature of 70-75°C (total concentration ratio is 22 times), so that NaCl crystals continue to precipitate and crystals grow. ; The concentrated material is pumped into a conical blue centrifuge while it is hot to se...

Embodiment 3

[0039] A sausage casing factory enzymatically hydrolyzed heparin sodium to absorb the residual liquid, with a total solid content of 5.82%, organic matter 2.41%, NaCl 3.25%, and a gray-white suspension.

[0040] Use an ultrafiltration membrane with a molecular weight cut-off of 100,000 to filter the heparin adsorption residue in a cross-flow cycle. When the concentration of solids in the concentrated solution reaches 15.1%, the filter is stopped to obtain a gray concentrated solution and a transparent light yellow clear liquid; the clear liquid is evaporated by three effects , concentrated 14 times; the concentrated material was pumped into a decanter centrifuge while it was hot to separate NaCl crystals to obtain desalted mother liquor and NaCl crystals; washed with membrane filtrate of 1 times the quality of NaCl crystals to obtain NaCl crystals with a dry basis purity of 96%; desalted mother liquor Mix with the membrane filter concentrate, after testing, the protein content ...

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Abstract

The invention discloses a method for producing dried porcine solubles by using adsorption residual liquid obtained after extracting heparin by the enzymolysis method, and belongs to the technical field of environmental protection. The method comprises the following steps: taking adsorption residual liquid obtained after extracting heparin as a raw material; carrying out membrane filtration to obtain clear liquid and concentrated liquid; after evaporating, concentrating and crystallizing the clear liquid, separating and removing NaCl; mixing residual desalted mother liquor with the membrane filtration concentration liquid; after blending with auxiliary materials, drying to obtain the finished dried porcine soluble. The method can be used for purification treatment and recycling of casing-heparin waste water, the problem of waste water pollution caused by the high-COD and high-salt-content adsorption residual liquid obtained after extracting heparin by the enzymolysis method is solved, and meanwhile, the extraneous income of casing-heparin production enterprises is increased by recycling high-added-value by-products such as dried porcine soluble and NaCl.

Description

technical field [0001] The invention relates to a method for producing intestinal membrane protein powder by using an enzymatic hydrolysis method for heparin to absorb residual liquid, and belongs to the technical field of environmental protection. Background technique [0002] Casing-heparin processing wastewater is a pollutant produced during casing processing and heparin extraction. At present, there are two techniques for extracting heparin in China: salting-out method and enzymatic hydrolysis method. The salting-out method is to add salt and alkali to the intestinal mucosa to adjust the pH, raise the temperature to about 50°C and keep warm to dissolve the heparin and transfer it to water, then raise the temperature to 95°C to solidify the intestinal membrane protein, and then separate to obtain the by-product intestinal membrane slag (or called Intestinal membrane protein), and the separated supernatant is subjected to resin adsorption, analysis, alcohol precipitation,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23J1/00
CPCA23J1/002A23V2002/00A23V2250/542A23V2300/38A23V2300/10
Inventor 张建华李永富张宏建陈旭升王柯
Owner JIANGNAN UNIV
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