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A method for inducing tetraploid production of seedless Rosa roxburghii by mixed culture

A seedless prickly pear, tetraploid technology, applied in the direction of botanical equipment and methods, applications, plant genetic improvement, etc., to achieve the effect of increasing fruit diameter and edible parts, and increasing nutritional content

Inactive Publication Date: 2018-06-15
SOUTHWEST FORESTRY UNIVERSITY
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  • Description
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Problems solved by technology

For the improvement of seedless Rosa roxburghii varieties, only Wei Jingfeng et al. have carried out studies on single plant selection, but there has been no report on ploidy breeding

Method used

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  • A method for inducing tetraploid production of seedless Rosa roxburghii by mixed culture
  • A method for inducing tetraploid production of seedless Rosa roxburghii by mixed culture
  • A method for inducing tetraploid production of seedless Rosa roxburghii by mixed culture

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Embodiment Construction

[0017] In order to deepen the understanding of the present invention, the present invention will be further described below in conjunction with the accompanying drawings and embodiments, which are only used to explain the present invention and do not limit the protection scope of the present invention.

[0018] Example The seedless Rosa roxburghii sterile seedling established by the key laboratory of biodiversity conservation of a certain university was selected for polyploid induction test and ploidy identification

[0019] 1 Materials and methods

[0020] 1.1 Test material

[0021] As the test material, the aseptic seedlings of Rosa roxburghii established by the Key Laboratory of Biodiversity Conservation of Southwest Forestry University were selected.

[0022] 1.2 Test method

[0023] 1.2.1 Immersion method, see figure 2 shown

[0024] Remove the leaves and tops of the stems of sterile seedlings, cut them into about 1 cm long, keep at least one leaf axil for each segme...

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Abstract

The invention discloses a method for inducing generation of Rosa sterilis tetraploid through a mixed culture method. The method comprises the following steps: Step 1, material preparation: aseptic seedling stem is cut into sections of 0.8-3 cm length with each section at least having a leaf axil; Step 2, preculture: preculture is carried out in a medium MS+0.5 mg / L 6-BA for 0-3 d; Step 3, colchicines light culture: precultured stem sections are transferred into MS+0.5 mg / L 6-BA mediums with 0-200 mg / L of colchicines; Step 4, culture without colchicines: the stem sections are transferred into MS+0.5 mg / L 6-BA mediums without colchicines to be continuously cultured after 7 d of culture in the Step 3; and Step 5, homogenization treatment of variation buds: after 40 d of culture in the Step 4, cultured buds-seedlings with obvious variation is repeatedly cultured in the medium MS+0.15 mg / L NAA+0.5 mg / L 6-BA for five times at 40 d interval. By the induction method, a tetraploid Rosa sterilis new germplasm is obtained. Nutrient components such as Vc, VE and SOD, etc. of Rosa sterilis fruits can be increased as well as fruit width and edible parts.

Description

technical field [0001] The invention relates to the technical field of a method for producing polyploids of Rosa roxburghii by mixed cultivation, in particular to a method for inducing tetraploids of Rosa roxburghii by mixed cultivation. Background technique [0002] Seedless prickly pear (Rosa sterilis S.D.Shi) is a perennial climbing shrub belonging to the genus Rosa of the family Rosaceae. It is one of the endemic species in China, and it is a relative species of prickly pear (R.roxbunghii). Its wild resources are mainly distributed in the north of Anshun City, Guizhou Province and the south of Guiyang City. In recent years, a large number of species have been introduced and planted in many places. Seedless Rosa roxburghii has high medicinal health care, sightseeing, ecological protection and economic value. Compared with common prickly pears, the single fruit of seedless prickly pears is seedless or has 1-2 seeds, less tannin content, higher sugar content, thick flesh ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H1/08
CPCA01H1/08
Inventor 辛培尧辛亚龙林源唐军荣李斌何承忠韩国伟
Owner SOUTHWEST FORESTRY UNIVERSITY
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