Rice blast resistance gene, isolation and cloning method, molecular marker and application

Abstract

The invention discloses a rice blast resistance gene, an isolation and cloning method, a molecular marker and an application. An F2 group is constructed by taking a broad-spectrum rice blast highly resisting conventional rice variety, namely Zixuan No.2, as a donor and a rice blast sensitive rice variety, namely Lijiang Xintuan Heigu LTH, as a receptor through forward and reverse hybridization. By virtue of a map-based cloning method, a target rice blast resistance gene containing an NB-ARC structural domain is located; the target gene just keeps difference of only one amino acid between resistant and susceptible parents and the difference is located on a phosphate binding loop P-loop of the NB-ARC structural domain; the gene is determined as a rice blast resistance gene Pitb; and finally, in accordance with the difference of the resistant and susceptible genes, the corresponding molecular marker is developed. The rice blast resistance gene Pitb, when transferred to rice blast susceptible rice variety, is conducive to the generation of a novel rice blast resistance rice variety. The molecular marker of the rice blast resistance gene Pitb can greatly improve the efficiency of applying the gene to breeding selection and shorten a breeding time.

Description

technical field [0001] The invention relates to the fields of molecular biology technology and breeding, in particular to a rice blast resistance gene Pitb, a separation and cloning method, a molecular marker and its application in rice improvement. Background technique [0002] Rice blast, caused by Magnaporthe grisea, is one of the most devastating fungal diseases in rice cultivation and a worldwide fungal disease (Okuyama et al., 2011; Inoue et al., 2013). Rice blast has been reported in more than 80 countries so far, among which Asia and Africa are the most serious. According to incomplete statistics, rice blast causes 11% to 30% reduction in rice production globally every year, with an annual average of more than 10 million tons. , seriously threatening the world's food production (Wilson et al., 2009; Zhai et al., 2011; Xu et al., 2014). At present, cultivating and promoting the application of resistant varieties is the most cost-effective and environmentally safe mea...

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Problems solved by technology

[0007] Traditional breeding methods rely on resistance identification and phenotypic selection, which not only have a long cycle and low efficiency, but also are limited by many conditions, making it difficult to meet the production requirements in breeding new rice blast-resistant varieties (Chen Dexi et al., 2010)

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