Method of molecular marker assisted backcross to improve scab invasion resistance of wheat

A technology assisted by molecular markers and head blight, which is applied in the fields of botany equipment and methods, biochemical equipment and methods, and microbial measurement/inspection, etc. Improve the efficiency of breeding selection, accelerate the speed of promotion, and reduce the cost

Inactive Publication Date: 2013-05-29
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, using the traditional backcross breeding method to improve wheat scab resistance is time-consuming and laborious, which seriously hinders the effective use of resistance sources in scab-resistant breeding.

Method used

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  • Method of molecular marker assisted backcross to improve scab invasion resistance of wheat
  • Method of molecular marker assisted backcross to improve scab invasion resistance of wheat
  • Method of molecular marker assisted backcross to improve scab invasion resistance of wheat

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] Example 1 Using SSR markers closely linked to the target QTL to assist backcrossing to improve the invasion resistance of wheat lines

[0057] F 1 ; Then use Mianyang 99-323 as the reincarnation parent to backcross and get BC 1 f 1 . Will BC 1 f 1 The isolated population is planted in the field as a single plant, listed at the seedling stage, and the DNA of each individual plant is extracted, and the genotype is analyzed using the SSR markers closely linked to the target QTL, and the heterozygous individual plants carrying the target QTL are selected to continue backcrossing to obtain BC 2 f 1 . Use a similar method to select individual plants and continue backcrossing to obtain BC 3 f 1 . in bc 3 f 1 In the segregation population, the homozygous individual plants carrying the target QTL were selected according to the marker genotypes to obtain near-isogenic lines by selfing. A total of 1 generation of crossing, 3 generations of backcrossing and 2 generation...

Embodiment 2

[0068] Example 2 Select SSR markers throughout the wheat genome to analyze the background recovery rate

[0069] In order to speed up the restoration of recurrent parents in areas other than the target QTL, the genetic map constructed using the Nanda 2419×Wangshuibai recombinant inbred line population (Xue SL, Zhang ZZ, Lin F, Kong ZX, Cao Y, Li CJ, Yi HY, Mei MF, Zhao DM, Zhu HL, Xu HB, Wu JZ, Tian DG, Zhang CQ, Ma ZQ (2008) A high-density intervarietal map of the wheat genome enriched with markers derived from expressed sequence tags Theor Appl Genet 117:181-189) a marker was selected every 20-30cM, and a total of 150 pairs of SSR markers distributed on 21 chromosomes were selected for the background recovery rate analysis of each generation.

[0070] Results show: in BC 2 f 1 In the first generation, 93 pairs of markers were randomly selected from 150 pairs of markers to evaluate the genetic background of the individual plants with the target QTL, and the individual plant...

Embodiment 3

[0071] Example 3 Field Identification of Scab Resistance and Invasiveness of Breeding Near-Isogenic Lines

[0072] The field resistance evaluation was carried out in the Jiangpu experimental field of Nanjing Agricultural University from 2008 to 2011. Random block design was adopted, with 2 rows per plot, row length 1.5m, row spacing 0.25m, plant spacing 0.1m, and 15 plants per row. Inoculation was carried out two weeks before flowering by spreading diseased grains infected with Gibberella to the field. The Gibberella used was a mixture of strong pathogenic strains F4, F15, F17 and F34. 15 days after flowering, the diseased ear rate (PIS) or diseased spikelet rate (PDS) was investigated, and the resistance to head blight invasiveness of the bred near-isogenic lines was evaluated.

[0073] The results showed that compared with the recurrent parents, the diseased panicle rate and diseased spikelet rate of the near-isogenic lines were significantly reduced. Due to the existence...

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Abstract

The invention discloses a method of molecular marker assisted backcross to improve scab invasion resistance of wheat. The method uses a recombinant inbred line carrying an invasion resistant QTL as a donor and a scab infected line with good integral property as a receptor to carry out hybridization and backcross. In each backcross generation, flanking markers closely linked with target QTL are used to carry out foreground selection, while 150 pairs of SSR markers distributed in the wheat genome are used to carry out background selection on single plant with target QTL; single plant with the highest background recovery rate is selected for the backcross of a next generation. After continuous backcross for three generations and inbred for two generations, a coisogenic strain with obviously improved resistance and other characters substantially consistent with the recurrent parent can be acquired. The method of the present invention extracts leaf DNA of the backcross progeny in seedling stage, and carries out marker genotype analysis to gain the target plant, so as to effectively solve the problems that scab resistance in phenotypic selection is susceptible the effects of environmentconditions and that resistance phenotype can not be identified until flowering stage; while the method also reduces the population size, lowers breeding cost, and improves scab invasion resistance ofwheat strains fast and effectively.

Description

1. Technical field [0001] The invention discloses a method for rapidly improving the resistance to scab invasiveness of wheat by molecular marker-assisted backcrossing, which belongs to the field of crop molecular breeding and is specially used for the selection and identification of germplasm resistant to scab invasiveness of wheat. 2. Background technology [0002] Wheat is one of the most important food crops in the world, and its products are indispensable to human food security. Head blight caused by Fusarium graminearum Schwabe is an important disease in wheat production, which widely occurs in warm, humid and semi-humid regions. The hazards include sterility and reduced seed plumpness, resulting in a severe drop in yield, generally by 10-15%, sometimes as high as 50%. Diseased wheat grains also contain toxins (mainly deoxynivalenol, DON), which will directly endanger the health of humans and animals after consumption. Due to global warming and changes in farming sys...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68A01H1/02
Inventor 马正强薛树林李国强许峰
Owner NANJING AGRICULTURAL UNIVERSITY
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