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Stem cell preparation for treating liver cirrhosis

A stem cell preparation and stem cell technology, applied in the field of stem cells and tissue engineering, can solve the problems of difficult culturing and preservation of liver cells, low proliferation of liver cells, and difficulty in achieving clinical treatment, etc. The effect of treating liver cirrhosis

Inactive Publication Date: 2016-11-16
SHEN ZHEN ISTEM REGENERATIVE MEDICINE SCI TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, hepatocytes are a kind of static cells, and the number of transplanted hepatocytes proliferates is small, so it is difficult to achieve the effect of clinical treatment, and it is difficult to culture and preserve hepatocytes.

Method used

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  • Stem cell preparation for treating liver cirrhosis
  • Stem cell preparation for treating liver cirrhosis
  • Stem cell preparation for treating liver cirrhosis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] 1. Preparation of mesenchymal stem cells

[0024] Source of material: Human bone marrow mesenchymal stem cells were derived from a 61-year-old male patient with esophageal cancer surgery, provided by the Department of Cardiothoracic Surgery, Shenzhen First People's Hospital. The patient gave informed consent to the treatment and experiment, and the experimental protocol was approved by the hospital's medical ethics committee.

[0025] Isolation and culture of human bone marrow mesenchymal stem cells: 2 mL of bone marrow was obtained from the resected ribs of patients with esophageal cancer, anticoagulated with heparin, transferred to the upper layer of a centrifuge tube containing the same volume of Percoll lymphocyte separation medium, and centrifuged at 2000 r / min for 10 min with a density gradient. Take the middle mononuclear cell layer, wash it twice with PBS, add DMEM medium containing 20% ​​fetal bovine serum by volume fraction, and adjust the cell concentration to...

Embodiment 2

[0033] 1. Preparation of mesenchymal stem cells

[0034] Please refer to Embodiment 1, which is the same as Embodiment 1.

[0035] 2. Preparation of stem cell preparations

[0036]The third-generation human bone marrow mesenchymal stem cells prepared in step 1 were 1×10 8 1, mixed with IL-18 binding protein 0.002mg, oxymatrine 0.2mg, and appropriate amount of water to prepare 20ml stem cell preparation. Furthermore, the concentration of mesenchymal stem cells was 5×10 6 One, the concentration of IL-18 binding protein was 0.1 mg / l, and the concentration of oxymatrine was 10 mg / l.

Embodiment 3

[0038] 1. Preparation of mesenchymal stem cells

[0039] Please refer to Embodiment 1, which is the same as Embodiment 1.

[0040] 2. Preparation of stem cell preparations

[0041] The third generation human bone marrow mesenchymal stem cells prepared in step 1 were 2×10 8 1, mixed with 0.02 mg of IL-18 binding protein, 2 mg of oxymatrine, and an appropriate amount of water to prepare a 20 ml stem cell preparation. Furthermore, the concentration of mesenchymal stem cells was 1×10 7 Each, the concentration of IL-18-binding protein was 1 mg / l, and the concentration of oxymatrine was 100 mg / l.

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Abstract

The invention discloses a stem cell preparation for treating liver cirrhosis. The stem cell preparation for treating liver cirrhosis comprises mesenchymal stem cells, IL-18 binding protein, oxymatrine and solvent, wherein the concentration of the mesenchymal stem cells is (5-10)*106 / ml; the concentration of the IL-18 binding protein is 0.1-1 mg / L; the concentration of the oxymatrine is 10-100 mg / L. Through the stem cell preparation for treating liver cirrhosis, the liver inflammatory degree can be effectively decreased, liver fibrosis is reversed, liver tissue is restored, liver function restoration is promoted, and formation of liver fibrosis is effectively inhibited.

Description

technical field [0001] The invention relates to the technical field of stem cells and tissue engineering, in particular to a stem cell preparation for treating liver cirrhosis. Background technique [0002] Cirrhosis is a troublesome liver disease. Although liver transplantation is an effective treatment for advanced liver cirrhosis, the prognosis of these patients is still poor; and there are problems such as lack of donors, surgical injury, immune rejection and high cost of liver transplantation. Hepatic cell transplantation is considered as one of the alternative methods of organ transplantation, not only can temporarily maintain the liver function of patients waiting for liver transplantation, but also can treat certain liver metabolic diseases. However, hepatocytes are a kind of quiescent cells, and the number of transplanted hepatocytes proliferates is small, so it is difficult to achieve the effect of clinical treatment, and it is difficult to culture and preserve he...

Claims

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Application Information

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IPC IPC(8): A61K35/28A61K38/20A61P1/16A61K31/4375
CPCA61K35/28A61K31/4375A61K38/20A61K2300/00
Inventor 曾宪卓鲁菲
Owner SHEN ZHEN ISTEM REGENERATIVE MEDICINE SCI TECH CO LTD