Rapid microRNA-1 detection method

Abstract

The invention relates to a rapid microRNA-1 detection method and belongs to the technical field of biological detection. The rapid microRNA-1 detection method comprises the steps that a single-stranded DNA sequence completely complementary with a target microRNA-1 to be detected is designed according to a sequence of the target microRNA-1 to be detected, then two ends of the single-stranded DNA sequence are respectively modified with a fluorophore and a fluorescence quenching group, accordingly a Taqman probe corresponding to the target microRNA-1 to be detected is obtained, then serum, RNase-free water, DSN enzyme, the Taqman probe and an RNA enzyme inhibitor of a patient react in certain proportion under the specific conditions, and the fluorescence intensity of reaction liquid is detected under the excitation of laser, namely a detection result of the target microRNA-1 to be detected is obtained. By the adoption of the detection method, the detection time of a myocardial damage marker microRNA-1 is shortened greatly, and the detection efficiency is improved based on detection quality guarantee.

Description

technical field [0001] The invention relates to the field of biological detection, in particular to a rapid detection method for microRNA-1. Background technique [0002] Acute myocardial infarction is myocardial necrosis caused by acute and persistent coronary ischemia and hypoxia. Clinically, there are often severe and persistent substernal pains, which cannot be completely relieved by rest and nitrates, accompanied by increased serum myocardial enzyme activity and progressive ECG changes, which may be complicated by arrhythmia, shock or heart failure, often life-threatening. The disease is most common in Europe and the United States, and about 1.5 million people in the United States suffer from myocardial infarction every year. China has shown a clear upward trend in recent years, with at least 500,000 new cases and at least 2 million existing cases each year. [0003] It can be seen that the early detection of acute myocardial infarction is particularly important. In t...

AI Technical Summary

Problems solved by technology

[0005] In view of the above-mentioned problems, the present invention provides a rapid detection method of microRNA-1, which is applied to the detection of myocardial injury markers of acute myocardial infarction, so as to overcome the myocardial injury of microRNA-1 detected by the detection method in the prior art. The markers lead to a long detection time and a large workload, which greatly shortens the detection time of microRNA-1, a myocardial injury marker, and simplifies the detection method of the above-mentioned microRNA-1, a myocardial injury marker. On the basis of quality, the detection efficiency is improved

Images