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A special organophosphorus decomposing bacterium yp8 for tea trees and preparation method thereof

A technology of organophosphorus and decomposing bacteria, which is applied in the fields of biochemical equipment and methods, organic fertilizers, chemical instruments and methods, etc., and can solve the problems of low absorption of crops and accumulation of phosphorus elements.

Active Publication Date: 2018-11-30
SHANDONG AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For a long time, people have used a large amount of chemical fertilizers and pesticides in order to increase the output of agricultural products. Phosphorus fertilizers are often quickly fixed to form ineffective phosphorus after application, resulting in low absorption of crops and a large accumulation of phosphorus in the soil.

Method used

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  • A special organophosphorus decomposing bacterium yp8 for tea trees and preparation method thereof
  • A special organophosphorus decomposing bacterium yp8 for tea trees and preparation method thereof
  • A special organophosphorus decomposing bacterium yp8 for tea trees and preparation method thereof

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Effect test

Embodiment 1

[0014] Embodiment 1, take the separation and identification of special organophosphate-decomposing bacteria for tea trees as an example

[0015] The inventors of the present invention collected soil samples from tea gardens in Shandong, placed them in sterile bags for low-temperature preservation, and brought them back to the laboratory for preservation at 4°C. Using organic phosphorus medium (glucose 10g, (NH 4 ) 2 SO 4 0.5g, NaCl 0.3g, KCl 0.3g, FeSO 4 ·7H 2 O0.03g, MgSO 4 ·7H 2 O 0.3g, MnSO 4 4H 2 O 0.03g, CaCO 3 5g, appropriate amount of lecithin, dilute to 1000mL, adjust the pH to 7.0-7.5) Screen the target strain from the soil. Combining traditional morphological, physiological and biochemical characteristics with PCR-based modern technology, the cultured strains were classified and identified, and finally an organophosphate-decomposing bacterium was screened out.

[0016] After morphological observation (such as figure 1 ), it was found that the colony of t...

Embodiment 2

[0020] Embodiment 2, prepare a kind of special organophosphorus decomposing bacteria bacterial agent of tea tree

[0021] (1) Preparation of solid composite carrier: mix wheat bran, peat, and diatomaceous earth in a mass percentage of 70%: 20%: 10%, then add distilled water (distilled water) of 1.5 times the total volume of wheat bran, peat, and diatomite pH=7), the solid composite carrier was obtained after high temperature sterilization at 120°C;

[0022] (2) Preparation of bacterial liquid seeds: first pick Pseudomonas putida YP8 and inoculate it into 100mL LB liquid medium (tryptone 10g, yeast extract 5g, sodium chloride 10g), and culture at 30°C with shaking, Cultivate until the OD value of the bacterial solution = 0.5. Then, the culture solution and the LB liquid medium that reached OD value = 0.5 were mixed according to the volume ratio of 1:25, and cultured with shaking at 30° C. for 24 hours to obtain the liquid seeds of the strain.

[0023] (3) inoculate the liquid...

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Abstract

The present invention relates to a special organophosphorus decomposing bacterium YP8 for tea trees and a preparation method thereof; an organophosphorus decomposing bacterium YP8 with a preservation number of GDMCC NO: 60053 was isolated from the soil of a tea garden in Shandong; it was published on June 29, 2016 It was preserved in the Guangdong Microbial Culture Collection Center, and its 16S rDNA sequence is shown in SEQ.ID.NO 3; the special organic phosphorus decomposing bacteria agent YP8 for tea trees prepared by using this strain can effectively increase the available phosphorus content in tea garden soil.

Description

1. Technical field [0001] The invention relates to a special organophosphorus decomposing bacterium YP8 for tea trees and a preparation method thereof, belonging to the field of biotechnology. 2. Background technology [0002] Phosphorus is a major nutrient element for plant growth and exists mainly in the form of insoluble minerals in the soil. For a long time, people have used a large amount of chemical fertilizers and pesticides in order to increase the output of agricultural products. Phosphorus fertilizers are often quickly fixed to form ineffective phosphorus after application, resulting in low absorption of crops and a large accumulation of phosphorus in the soil. There has been a lot of research on improving phosphorus utilization in plants, among which the role of microorganisms capable of dissolving refractory phosphorus is considered to be crucial. It is a good way to solve the problem of plant phosphorus utilization by screening out microorganisms with phosphoru...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C09K17/14C12R1/40
CPCC09K17/14C12N1/20C12N1/205C12R2001/40
Inventor 韩晓阳张丽霞周波黄晓琴向勤锃
Owner SHANDONG AGRICULTURAL UNIVERSITY