Construction method of animal model for biliary atresia of chronic liver disease

Abstract

The invention relates to a construction method of an animal model for biliary atresia of chronic liver disease firstly utilizing a Gr-1 antibody to construct the construction method of the animal model for biliary atresia of chronic liver disease. The method comprises following steps: injecting rhesus monkey rotavirus within the 24 hours of neonatal mouse to induce a classic animal model for biliary atresia, firstly injecting the Gr-1 antibodies before rhesus monkey rotavirus is injected; injecting the Gr-1 antibodies multiple times after mouse are born on the 14th day to maintain the effective concentration range of the Gr-1 antibodies in the mouse; and injecting 5-20ng / 1.6g of Gr-1 antibodies to the mouse each time. The construction method of an animal model for biliary atresia of chronic liver disease has following beneficial effects: the model is capable of simulating the process of biliary atresia, development and prognosis, especially in the end-stage hepatocirrhosis phase.

Description

technical field [0001] The present invention relates to the technical field of animal model construction, and more specifically, relates to a method for constructing an animal model of chronic liver cirrhosis and biliary atresia. Background technique [0002] Biliary atresia (BA) is a progressive inflammatory cholangiopathy that occurs in infants and young children. It is a common disease that causes obstructive jaundice in infants and young children, and eventually leads to obstruction of extrahepatic and intrahepatic bile ducts and liver cirrhosis. The prognosis is poor and death occurs. High rate. In view of the ambiguity and complexity of the etiology of biliary atresia, the clinical research on biliary atresia is limited. People often construct animal models to simulate the symptoms of biliary atresia, so as to study its pathogenesis and try to find a way to treat the disease. [0003] Rhesus monkey rotavirus (rhesus rotavirus group A, RRV) is considered to have a cert...

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Problems solved by technology

However, there are still many defects in the development of this model: 1. In the classic animal model of biliary atresia induced by injection of RRV within 24 hours after birth, although the acute inflammatory stage of biliary atresia can be simulated, newborn mice can show systemic jaundice, Coarse hair, low body weight, dark yellow urine, white clay-like stool, poor mental state, etc. However, the mice in this model could not survive to 14 days due to the strong immune response of the biliary system of newborn mice to RRV , the survival rate is extremely low; 2. In the animal model of biliary atresia induced by injection of high doses of RRV on the 4th or 5th day after birth, although the mice can survive to 15-20 days, the model is unstable and the repetition rate Low; the dose of injected virus stock solution is large, resulting in waste; most of the mice in this model showed symptoms of transient hepatitis, which did not conform to the clinical characteristics of patients with biliary atresia; according to anatomical observation, biliary atresia rarely occurred in this model; The model still cannot fully simulate the stage of biliary atresia in end-stage cirrhosis; therefore, the existing rotavirus-induced biliary atresia animal model can only simulate the acute inflammatory stage of clinical biliary atresia patients, and there is no sufficient evidence that the existing model Can simulate the stage of chronic cirrhosis or end-stage cirrhosis with biliary atresia

However, the existing animal models of biliary atresia cannot simulate the occurrence, development, and prognosis of the entire disease process of biliary atresia.

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