Gentiana veitchiorum effective part and preparation method and application thereof

A technology of blue hosta gentian and effective parts, applied in the field of plant extracts, can solve problems such as uncontrollable cell growth and transfer, cell cycle disorder, etc., and achieve the effects of convenient extraction and high extraction efficiency

Inactive Publication Date: 2016-12-07
QINGHAI UNIV FOR NATITIES
View PDF0 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, although different cancers may have different carcinogenic causes, their histological observations all show similar cell cycle disorders and rapid and uncontrollable cell growth and metastasis

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Embodiments 1 to 3 are the specific implementations of the effective fraction of Gentiana gentiana of the present invention and the preparation method thereof, wherein embodiment 1 is the best embodiment.

[0022] Example 1

[0023] The preparation method comprises the following steps: pulverize the whole plant of Gentiana gentianus, use an ethanol solution with a mass percentage concentration of 75% to 85% for ultrasonic extraction for 1 hour, and filter to obtain an ultrasonic extraction solution and filter residue, and add the filter residue with a mass percentage concentration of 75% to 85%. 85% ethanol solution was refluxed and extracted 3 times, each time for 0.5 to 1 hour, to obtain a refluxed extract, combined the sonicated extract and the refluxed extract, concentrated and dried to obtain an extract; the extract was dispersed with distilled water to obtain an extract dispersion, and then Sequentially using petroleum ether, ethyl acetate and n-butanol as solvents ...

Embodiment 2

[0025] The preparation method comprises the following steps: pulverize the whole plant of Gentiana gentiana, use an ethanol solution with a mass percentage concentration of 85% to 95% for ultrasonic extraction for 3 hours, and filter to obtain an ultrasonic extract and filter residue, and add the filter residue with a mass percentage concentration of 85% to 95%. Reflux extraction with 95% ethanol solution twice, each time for 1.5 to 2 hours, to obtain a reflux extract, combine the sonic extract and reflux extract, concentrate and dry to obtain an extract; disperse the extract with distilled water to obtain an extract dispersion, and then Sequentially using petroleum ether, ethyl acetate and n-butanol as solvents to extract the extract dispersion, volatilize and remove the solvent to obtain the petroleum ether part of Gentiana japonica, the ethyl acetate part of gentiana japonica, and the n-butanol part of gentiana japonica; After extracting the extract dispersion, the remaining...

Embodiment 3

[0027] The preparation method comprises the following steps: pulverize the whole plant of Gentiana gentiana, use an ethanol solution with a mass percentage concentration of 65% to 75% for ultrasonic extraction for 2 hours, filter to obtain an ultrasonic extraction solution and filter residue, and add the filter residue with a mass percentage concentration of 65% to 75%. 75% ethanol solution reflux extraction twice, each time for 1 to 1.5 hours, to obtain the reflux extract, combine the sonic extract and the reflux extract, concentrate and dry to obtain the extract; disperse the extract with distilled water to obtain the extract dispersion, and then Sequentially using petroleum ether, ethyl acetate and n-butanol as solvents to extract the extract dispersion, volatilize and remove the solvent to obtain the petroleum ether part of Gentiana japonica, the ethyl acetate part of gentiana japonica, and the n-butanol part of gentiana japonica; After extracting the extract dispersion, th...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a gentiana veitchiorum effective part and a preparation method and application thereof, and belongs to the technical field of plant extracts. The effective part is selected from one of or a mixture of two or more of, by any mass ratio, a gentiana veitchiorum petroleum ether part, a gentiana veitchiorum ethyl acetate part, a gentiana veitchiorum n-butanol part and a gentiana veitchiorum aqueous phase extraction part. A whole gentiana veitchiorum plant is smashed, an ethyl alcohol solution with the volume percentage concentration being 65-95% is added for reflux extraction, and the obtained concentrated solution is concentrated and the concentrate is dried to obtain an extract; distilled water is added to the extract for dispersion to obtain an extract dispersion solution, petroleum ether, ethyl acetate and n-butanol sequentially serve as solvents to extract the extract dispersion solution, and the gentiana veitchiorum petroleum ether part, the gentiana veitchiorum ethyl acetate part and the gentiana veitchiorum n-butanol part are obtained after the solvents are volatilized; the extracted extract dispersion solution is concentrated and the concentrated material is dried to obtain the gentiana veitchiorum aqueous phase extraction part. The gentiana veitchiorum effective part is applied to preparing medicine for inhibiting CDC25 enzyme, and can be used for preparing anti-tumor medicine.

Description

technical field [0001] The invention relates to effective parts of blue hosta gentian and its preparation method and application, and belongs to the technical field of plant extracts. Background technique [0002] blue hosta gentian Corydalis melanochlora Maxim. is a plant of the genus Corydalis in the family Papaveraceae, a hairless herb [see: Flora of China. Volume 32. Beijing: Science Press, 1999: 266]. Also known as "Maiqiangri, Erwa, and Yinzhou Seerwa", it is distributed in Gansu (Sunan, Tianzhu, Minxian, Diebu), eastern Qinghai (Qilian, Datong, Guinan, Zeku, Tong Germany, Henan), Sichuan and other places, born in alpine meadows or flowstone beaches at an altitude (2850-5500) meters. Blue hosta gentian is a commonly used traditional Chinese medicine, which has been recorded in herbal medicines of past dynasties. It can clear away heat and gallbladder, clear away heat and detoxify. Treatment of diseases of the cardiovascular system, sepsis, externally used for wound...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/66A61P35/00
CPCA61K36/66A61K2236/333A61K2236/39A61K2236/51A61K2236/53
Inventor 芦永昌吴疆白雪钱帅李海丽朱奎德曾擎屹郑虹田静王欢
Owner QINGHAI UNIV FOR NATITIES
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap