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Ruminant clostridial disease quadruple inactivated vaccine and preparation method thereof

A technology of inactivated vaccines and ruminants, which can be applied to antibacterial drugs, bacterial antigen components, etc., and can solve the problem of low success rate of treatment

Inactive Publication Date: 2016-12-07
QILU ANIMAL HEALTH PROD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because the treatment success rate is very low, the focus of the disease is on prevention. Wide-scale vaccination can effectively reduce the losses caused by the disease. At present, there are no reports on the development of vaccines that can prevent the above four clostridial diseases with one immunization

Method used

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  • Ruminant clostridial disease quadruple inactivated vaccine and preparation method thereof
  • Ruminant clostridial disease quadruple inactivated vaccine and preparation method thereof
  • Ruminant clostridial disease quadruple inactivated vaccine and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] ——Preparation of quadruple inactivated vaccine against clostridial disease in ruminants

[0055] 1. Strains of Clostridium emphysema C54-2, Clostridium putrefaction C55-1, C-type Clostridium perfringens C59-2 and D-type Clostridium perfringens C60-2 (see Please see China Veterinary Drug Supervision Institute, China Veterinary Microbiological Strain Preservation Management Center edited, Chinese Veterinary Strain Catalog (Second Edition), China Agricultural Science and Technology Press, 2002, p40, 52, 48, 50) strains are all purchased From the Bacteria and Virus Preservation Center of the China Veterinary Drug Control Institute, it is used as a production strain.

[0056] 2. Culture medium Clostridium putrefaction uses trypsin to digest beef broth, Clostridium perfringens uses meat liver and stomach enzyme digestion soup (or cod liver and stomach enzyme digestion soup), Clostridium emphysema uses anaerobic meat liver soup, All were purchased from Rongcheng Yulin Biologi...

Embodiment 2

[0071] —— Vaccine Safety Trials

[0072] (1) Three components of Clostridium putrefaction, Clostridium perfringens type C and Clostridium perfringens type D

[0073] Inoculate 5 2kg rabbits with the prepared vaccine, each subcutaneously inject 2ml (equivalent to 5 sheep immunization doses), observe for 8 days, and all 5 rabbits are healthy and alive.

[0074] (2) Emphysema part

[0075] 2 guinea pigs with a body weight of 400 g were injected subcutaneously with 2 ml of vaccine (equivalent to 2 sheep immunization doses), observed for 8 days, and all 5 guinea pigs were healthy and alive.

[0076] The above results show that the quadruple inactivated vaccine for ruminant clostridial disease prepared by the invention meets the standards of safety inspection.

Embodiment 3

[0078] - Vaccine efficacy test

[0079] (1) Parts of Clostridium putrefaciens, Clostridium perfringens type C and Clostridium perfringens type D

[0080] 1) Divide 30 rabbits with a body weight of 2kg into 6 groups, 5 in each group;

[0081] 2) Groups 1 to 3 were immunized by subcutaneous injection, and the immunization dose was 0.5ml, and groups 4 to 6 were not immunized and served as the control group;

[0082] 4) After 21 days of immunization, groups 1 and 4 were injected with 0.1ml (minimum lethal dose) of Clostridium putrefaction bacteria liquid, groups 2 and 5 were injected with 0.1ml (minimum lethal dose) of Clostridium perfringens toxin type C, 3, The 6 groups were injected with 0.1ml (minimum lethal dose) of D-type Clostridium perfringens poison.

[0083] 5) Observe for 5 days.

[0084] 6) The results determined that all the control rabbits died, and at least 4 immunized rabbits were qualified. The results are shown in Table 3.

[0085] Table 3 efficacy test resul...

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Abstract

The invention relates to a ruminant clostridial disease quadruple inactivated vaccine and a preparation method thereof. The ruminant clostridial disease quadruple inactivated vaccine is a clostridium putrificum, clostridium perfringens C type, clostridium perfringens D type and clostridium chauvoei quadruple inactivated vaccine. By use of the vaccine, four common cattle and sheep clostridial diseases can be subject to immunoprophylaxis, and the ruminant clostridial disease quadruple inactivated vaccine is used for preventing cattle and sheep blackleg (emphysematous gangrene), malignant oedema (clostridium septicum) and enterotoxaemia (C type and D type). Main ingredients of the vaccine are inactivated bacterial liquid of clostridium chauvoei and clostridium septicum, C type clostridium perfringens toxin and D type clostridium perfringens toxin.

Description

technical field [0001] The invention relates to a quadruple inactivated vaccine against ruminant clostridial disease and a preparation method thereof. It is a quadruple inactivated vaccine for preventing clostridial diseases of ruminants such as cattle and sheep caused by Clostridium putrefaciens, Clostridium perfringens type C, Clostridium perfringens type D and Clostridium aeruginosa. its preparation method. It belongs to the field of veterinary biological products. Background technique [0002] Clostridium is a general term for diseases caused by pathogenic species of Clostridium. Clostridia are anaerobic bacteria, there are more than 60 species, common pathogenic bacteria are only more than 10 species, mostly present in soil, sewage, and human and various animal feces. Gram staining is positive, except for a few species, all have flagella, can move and form spores, and their diameter is larger than that of bacteria. Most bacterial species can produce severe exotoxins...

Claims

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Application Information

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IPC IPC(8): A61K39/116A61K39/08A61P31/04
Inventor 鞠洪涛王蕾吴昊马宗耀禚宝山
Owner QILU ANIMAL HEALTH PROD
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