A kind of examination method of cerebroprotein hydrolyzate oral liquid
A cerebroprotein hydrolysate and testing method technology, applied in instruments, measuring devices, scientific instruments, etc., can solve problems such as poor accuracy and repeatability, and achieve rapid and accurate testing, high testing precision, and good repeatability Effect
Active Publication Date: 2018-09-21
吉林万通药业集团梅河药业股份有限公司
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Problems solved by technology
[0006] In view of the above-mentioned deficiencies in the prior art, the object of the present invention is to provide a test method for cerebroprotein hydrolyzate oral liquid, aiming at solving the problem of poor accuracy and repeatability of the existing cerebroprotein hydrolyzate oral liquid test method
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[0022] The present invention provides a test method for cerebroprotein hydrolyzate oral liquid. In order to make the purpose, technical scheme and effect of the present invention clearer and clearer, the present invention will be further described in detail below. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.
[0023] A kind of examination method of cerebroprotein hydrolyzate oral liquid provided by the present invention, it comprises steps:
[0024] S1. Prepare the derivatization reagent: draw 1.0ml of anhydrous acetonitrile and add it to the AQC reagent, then shake for 10 seconds, then put it into a 55°C oven and heat until the powder is completely dissolved to obtain the derivatization reagent;
[0025] S2. First put two 250μl lined tubes into two 2ml liquid phase sample bottles respectively, use a pipette gun to draw 10μl each of the amino acid reference solution and ...
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Abstract
The invention discloses a check method for a cerebroprotein hydrolysate oral solution. The check method includes the steps that firstly, a derivatization reagent is prepared, wherein 1.0 ml of anhydrous acetonitrile is sucked and added into an AQC reagent, the mixture is shaken for 10 s and then put into a 55 DEG C oven to be heated until the powder is completely dissolved, and the derivatization reagent is obtained; secondly, two 250 microliter liner tubes are put into two 2 ml liquid phase sample bottles respectively, 10 microliters of an amino acid reference substance solution and 10 microliters of the cerebroprotein hydrolysate oral solution are sucked with a pipette and put into the bottoms of the liner tubes, 70 microliters of a borate buffer solution is added into each liner tube, the liner tubes are put into a vortex oscillator for 1 min of vortex treatment, 20 microliters of the derivatization reagent is added into each liner tube, the liner tubes are put into the vortex oscillator for 5 min of vortex treatment, then placed for 10 min in a 55 DEG C oven and then taken out to be cooled to the room temperature, and 10 microliters of the mixture in each liner tube is sucked and injected into a liquid chromatograph for determination. By means of the check method, the type and content of amino acid can be checked; meanwhile, check precision is high, and repeatability is good.
Description
technical field [0001] The invention relates to the detection field, in particular to a detection method for cerebroprotein hydrolyzate oral liquid. Background technique [0002] "Brain protein hydrolyzate" is the purified extract of healthy pig brain tissue after enzymatic hydrolysis, mainly containing a variety of amino acids, neuropeptide genes, nucleotides, neurotransmitters, neurotrophic factors, cephalin, lecithin and other biological activities It is a substance that regulates nerve development, determines the differentiation of nerve cells and the direction of axon extension. "Brain protein hydrolyzate" can act on the central nervous system in various ways, regulate and improve the metabolism of neurons, promote the formation of synapses, induce the differentiation of neurons, and further protect nerve cells from various ischemia, Hypoxia and neurotoxin damage. The purified "brain protein hydrolyzate" easily passes through the blood-cerebrospinal fluid barrier, pro...
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IPC IPC(8): G01N30/02
CPCG01N30/02G01N2030/027
Inventor 滕红艳李子成孟昭敏孙楠张魏
Owner 吉林万通药业集团梅河药业股份有限公司