Effective part of corydalis hendersonii and preparation method and application of effective part of corydalis hendersonii

A technology of effective parts and parts, applied in the field of plant extracts, can solve the problems of research and application of anti-CDC25A and CDC25B enzymes that have not yet been seen, and achieve the effect of convenient extraction and high extraction efficiency

Inactive Publication Date: 2016-12-21
QINGHAI UNIV FOR NATITIES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are no reports on the research and application of Corydalis nepalensis and its effective parts against CDC25A and CDC25B enzymes

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Embodiments 1 to 3 are specific implementations of the effective fraction of Corydalis nepalensis and the preparation method thereof of the present invention, wherein embodiment 1 is the best embodiment.

[0023] Example 1

[0024] The preparation method comprises the following steps: pulverize the whole plant of Corydalis nepalensis, use an ethanol solution with a volume percentage concentration of 75-85% for ultrasonic extraction for 1 hour, and then filter to obtain an ultrasonic extraction liquid and a filter residue, and add the filter residue into an ethanol solution with a volume percentage concentration of 75-85% for reflux Extract 3 times, each time for 0.5 hours, to obtain the reflux extract, combine the acoustic wave extract and reflux extract, concentrate and dry to obtain the extract; disperse the extract with distilled water to obtain the extract dispersion, and then use petroleum ether, ethyl acetate Ester and n-butanol are used as solvents to extract the...

Embodiment 2

[0026] The preparation method comprises the steps of: pulverizing the whole plant of Corydalis nepalensis, ultrasonically extracting it with an ethanol solution with a concentration of 85-95% by volume for 3 hours, and then filtering to obtain an ultrasonic extraction liquid and filter residue, adding the filter residue to an ethanol solution with a concentration of 85-95% by volume to reflux Extract 3 times, each time for 1 hour, to obtain the reflux extract, combine the acoustic wave extract and reflux extract, concentrate and dry to obtain the extract; add distilled water to disperse the extract to obtain the extract dispersion, and then use petroleum ether, ethyl acetate Ester and n-butanol are used as solvents to extract the extract dispersion, and the ethyl acetate extract and the n-butanol extract are respectively evaporated to remove the solvents to obtain the ethyl acetate part of Nepalese Corydalis; after the extract dispersion is extracted, the remaining liquid is con...

Embodiment 3

[0028] The preparation method comprises the following steps: pulverize the whole plant of Corydalis nepalensis, use an ethanol solution with a volume percentage concentration of 65-75% for ultrasonic extraction for 0.5 hours, and then filter to obtain an ultrasonic extraction solution and a filter residue, and add the filter residue into an ethanol solution with a volume percentage concentration of 65-75% for reflux Extract twice, each time for 2 hours, to obtain the reflux extract, combine the acoustic wave extract and the reflux extract, concentrate and dry to obtain the extract; add distilled water to disperse the extract to obtain the extract dispersion, and then use petroleum ether, ethyl acetate Ester and n-butanol are used as solvents to extract the extract dispersion, and the ethyl acetate extract and the n-butanol extract are respectively evaporated to remove the solvents to obtain the ethyl acetate part of Nepalese Corydalis; after the extract dispersion is extracted, ...

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PUM

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Abstract

The invention belongs to the technical field of plant extracts and discloses an effective part of corydalis hendersonii and a preparation method and application of the effective part of corydalis hendersonii. The effective part of corydalis hendersonii is a mixture of one or more than two of an ethyl acetate part of corydalis hendersonii, an n-butyl alcohol part of corydalis hendersonii and an aqueous-phase extract part of corydalis hendersonii according to any mass ratio. The preparation method includes: grinding whole herbs of corydalis hendersonii, adding ethanol solution being 65%-95% in volume percent concentration to perform reflux extraction, and concentrating and drying extract liquor to obtain extract; adding distilled water into the extract to disperse to obtain extract dispersion liquid, sequentially adopting petroleum ether, ethyl acetate and n-butyl alcohol as solvents to extract the extract dispersion liquid, and volatilizing to remove the solvents to obtain the ethyl acetate part of corydalis hendersonii and the n-butyl alcohol part of corydalis hendersonii; concentrating and drying the extracted extract dispersion liquid to obtain the aqueous-phase extract part of corydalis hendersonii. Application of the effective part of corydalis hendersonii to preparation of CDC25 enzyme inhibition medicines is available for preparation of antitumor medicines.

Description

technical field [0001] The active part of Corydalis nepalensis and its preparation method and application belong to the technical field of plant extracts. Background technique [0002] Corydalis hendersonii Hemsl . is mainly distributed in the west of Xinjiang, west of Qinghai, and central to west of Tibet in my country. Kashmir and Nepal are also distributed. As a traditional Tibetan medicine "Sun stick", Corydalis nepalis is mainly used to treat fever and high blood pressure, and also has certain curative effect on hepatitis [see: Chinese Academy of Sciences. Northwest Plateau Institute of Biology. Tibetan Medicine [M]. Qinghai: Qinghai People's Publishing House, 1991: 350-351.]. This Tibetan medicinal material is rich in isoquinoline alkaloids [see: FU Y, ZHOU Y , LIAOX, et al. A new alkaloid from Cory dalis hendersonii [J]. Planta Medica, 2009, 75: 547-549 .]. "Tibetan Materia Medica" records that the whole herb treats vasculitis, blood fever, alpine plethora, enterit...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/66A61P35/00
CPCA61K36/66A61K2236/333A61K2236/51A61K2236/55
Inventor 林鹏程吴疆却生曾擎屹刘晓翠
Owner QINGHAI UNIV FOR NATITIES
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