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Corydalis pygmaea effective part, as well as preparation method and application thereof

A technology of effective parts and dwarf Viola, applied in the field of plant extracts, can solve problems such as not yet seen, and achieve the effects of convenient extraction, good anti-tumor effect, and improved extraction efficiency

Inactive Publication Date: 2016-12-21
QINGHAI UNIV FOR NATITIES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no report on the research and application of Corydalis dwarf Corydalis inhibiting the activity of HDAC1 enzyme

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Examples 1 to 3 are specific implementations of the effective parts of Corydalis dwarf and its preparation method of the present invention, and Example 1 is the best example.

[0024] Example 1

[0025] The preparation method includes the following steps: pulverize the whole Corydalis dwarf, ultrasonically extract with a 75%-85% ethanol solution by volume percentage for 1 hour, then filter to obtain the ultrasonic extract and filter residue, and add 75% volume percentage to the filter residue ~85% ethanol solution refluxed for 3 times, 0.5 hours each time, filtered to obtain refluxed extract, combined ultrasonic extract and refluxed extract, concentrated and dried to obtain extract; disperse extract with distilled water to obtain extract dispersion, then Petroleum ether, ethyl acetate and n-butanol are used as the solvent to extract the extract dispersion in sequence, and the petroleum ether extract and ethyl acetate extract are respectively used to volatilize the solvent to...

Embodiment 2

[0027] The preparation method includes the following steps: pulverizing the whole Corydalis dwarf, ultrasonically extracting with 85%-95% ethanol solution by volume percentage for 3 hours, then filtering to obtain the ultrasonic extract and filter residue, adding 85% volume percentage to the filter residue ~95% ethanol solution reflux extraction 3 times, 1 hour each time, filter to obtain reflux extract, combine ultrasonic extract and reflux extract, concentrate and dry to obtain extract; add distilled water to disperse extract to obtain extract dispersion, then Petroleum ether, ethyl acetate and n-butanol are used as the solvent to extract the extract dispersion in sequence, and the petroleum ether extract and ethyl acetate extract are respectively used to volatilize the solvent to obtain the dwarf corydalis petroleum ether part and the dwarf corydalis ethyl acetate part; After extracting the extract dispersion liquid, the remaining liquid is concentrated and dried to obtain th...

Embodiment 3

[0029] The preparation method includes the following steps: pulverize the whole Corydalis dwarf, ultrasonically extract with a volume percentage concentration of 65% to 75% ethanol solution for 0.5 hours, then filter to obtain the ultrasonic extract and filter residue, and add the volume percentage concentration of 65~ to the filter residue. 75% ethanol solution was refluxed for 2 times, 2 hours each time, filtered to obtain reflux extract, combined ultrasonic extract and reflux extract, concentrated and dried to obtain extract; disperse extract with distilled water to obtain extract dispersion, and then successively Use petroleum ether, ethyl acetate and n-butanol as solvents to extract the extract dispersion, take the petroleum ether extract and the ethyl acetate extract to volatilize the solvent respectively to obtain the dwarf corydalis petroleum ether part and the dwarf corydalis ethyl acetate part; After the extract dispersion liquid is extracted, the remaining liquid is c...

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PUM

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Abstract

The invention discloses a corydalis pygmaea effective part, as well as a preparation method and application thereof, and belongs to the technical field of a plant extract. The corydalis pygmaea effective part is mixture of one or more than one of a corydalis pygmaea petroleum ether part, a corydalis pygmaea ethyl acetate part and a corydalis pygmaea aqueous extraction part in an arbitrary mass ratio. The preparation method comprises the following steps: grinding whole grass of corydalis pygmaea, refluxing and extracting with an ethanol solution having a volume percentage concentration of 65-95 percent, concentrating and drying the extract to obtain an extract; dispersing the extract with distilled water to obtain an extract dispersant, extracting the extract dispersant sequentially with petroleum ether, ethyl acetate and n-butyl alcohol, and volatizing solvent to obtain a corydalis pygmaea petroleum ether part and a corydalis pygmaea ethyl acetate part; and concentrating and drying the extracted extract dispersant to obtain a corydalis pygmaea water-phase extraction part. The corydalis pygmaea effective part can be applied to preparation of medicines for inhibiting HDAC1 enzyme, and can be used for preparing anti-tumor medicines.

Description

Technical field [0001] The effective parts of Corydalis dwarf and its preparation method and application belong to the technical field of plant extracts. Background technique [0002] Corydalis hendersonii Hemsl (Corydalis hendersonii Hemsl) Tibetan medicine is called Sangezwa. The whole herb is used as a medicine to promote blood circulation, relieve blood stasis, promote qi and relieve pain, clear away heat and reduce inflammation. It is used by Tibetan medicine to treat high altitude hyperemia, gastric ulcer, gastritis and vasculitis , Enteritis, bruises, sciatica, scabies, venomous snake bites, knife wounds. [0003] Malignant tumors are one of the major diseases that seriously endanger human health and have become the primary cause of human death. The prevention and treatment of malignant cancer cells has become an important subject of concern in the medical field. However, the onset of malignant tumors is hidden, the etiology is complex, and the pathological changes are div...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/66A61P35/00
CPCA61K36/66A61K2236/333A61K2236/35A61K2300/00
Inventor 林鹏程吴疆却生曾擎屹刘晓翠
Owner QINGHAI UNIV FOR NATITIES
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