A kind of grape somatic embryo and its culture method, culture medium and application

A technology of somatic embryos and culture methods, which is applied in the field of grape tissue culture, can solve the problems of inability to guarantee the continuity of extraction work, low resveratrol content, and restrictions on the scale of industrial production of resveratrol

Active Publication Date: 2019-12-20
INST OF SPECIAL ANIMAL & PLANT SCI OF CAAS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The existing technology for extracting resveratrol mainly uses grape plants or grape fruits as raw materials for extraction, but the raw materials of this extraction method are limited by seasons and growth cycles, and the continuity of the extraction work cannot be guaranteed
Therefore, developed the use of grape callus as a raw material to extract the resveratrol contained therein, although the limitations of the growing season and cycle have been overcome, the existing culture methods cultivated and used as raw materials for extracting resveratrol The content of resveratrol in the callus of Vitis vinifera is low, which limits the industrial production scale of extracting resveratrol from the callus culture of Vitis vinifera

Method used

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  • A kind of grape somatic embryo and its culture method, culture medium and application
  • A kind of grape somatic embryo and its culture method, culture medium and application

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Experimental program
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Effect test

Embodiment 1

[0040] In this embodiment, the tender stem section of a variety of Vitis vinifera Zuoshan is used as the exosome of Vitis vitis vinifera, and the tender stem section of Vitis vitis vinifera is obtained from the National Fruit Tree Germplasm Zuojiashan Grape Resource Garden of the Institute of Special Products of the Chinese Academy of Agricultural Sciences. In other embodiments, the variety of Vitis vinifera is not limited to Zuoshanyi, and may be Beibinghong, Zuoyouhong, Shuanghong, Shuangfeng or Shuangyou and other varieties.

[0041] The culturing method of the mountain grape somatic embryo of the present embodiment comprises the steps:

[0042] (1) Rinse the taken tender stems of mountain grapes with tap water for 4 hours, then disinfect them with 70% alcohol for 30 seconds on an ultra-clean workbench, pour out the alcohol, disinfect them with 0.1% mercury liter for 5 minutes, and rinse them with sterile water for 3 minutes. ~4 times.

[0043] (2) Put the young grape stem...

Embodiment 2

[0051] The culturing method of the mountain grape somatic embryo of the present embodiment comprises the steps:

[0052] (1) Rinse the taken tender stems of mountain grapes with tap water for 4 hours, then disinfect them with 70% alcohol for 30 seconds on an ultra-clean workbench, pour out the alcohol, disinfect them with 0.1% mercury liter for 5 minutes, and rinse them with sterile water for 3 minutes. ~4 times.

[0053] (2) Put the tender stem section of Vitis vinifera into a petri dish, cut it into about 0.4 cm long stem section of Vitis vitis vinifera under aseptic conditions, that is, exosomes.

[0054] (3) Inoculate the cut vine stem sections into the Erlenmeyer flasks containing the pre-medium for pre-cultivation, inoculate 3 exosomes in each Erlenmeyer flask, and cultivate in dark under the pre-cultivation conditions at a temperature of 27°C 28 days to induce callus formation.

[0055] Wherein, the pre-medium used was prepared as follows: MS medium was used as the ba...

Embodiment 3

[0061] The culturing method of the mountain grape somatic embryo of the present embodiment comprises the steps:

[0062] (1) Rinse the taken tender stems of mountain grapes with tap water for 4 hours, then disinfect them with 70% alcohol for 30 seconds on an ultra-clean workbench, pour out the alcohol, disinfect them with 0.1% mercury liter for 5 minutes, and rinse them with sterile water for 3 minutes. ~4 times.

[0063] (2) Put the tender stem section of Vitis vinifera into a petri dish, and cut it into about 0.5 cm long section of Vitis vitis vinifera under aseptic conditions, that is, exosomes.

[0064] (3) Inoculate the cut vine stem sections into the Erlenmeyer flasks containing the pre-medium for pre-cultivation, inoculate 3 exosomes in each Erlenmeyer flask, and cultivate in dark under the pre-cultivation conditions at a temperature of 27°C 28 days to induce callus formation.

[0065] Wherein, the pre-medium used was prepared as follows: MS medium was used as the bas...

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Abstract

The invention discloses a grapevine somatic embryo and a culture method, culture medium and application thereof, belonging to the technical field of grape tissue culture. The invention selects and controls the carbon source, ventilation rate and nitrogen source of the somatic embryo induction medium, so that the growth of somatic embryos and the synthesis of resveratrol can reach the best state in the process of culturing, and the culturing obtained The somatic embryos have a relatively high content of resveratrol, and the method for culturing the grapevine somatic embryos of the present invention and the somatic embryos obtained by the method are the industrial production and production of the secondary metabolite resveratrol produced by the grapevine cell culture. Industrial development provides materials and basis.

Description

technical field [0001] The invention relates to the technical field of grape tissue culture, in particular to a grapevine somatic embryo and its culture method, culture medium and application. Background technique [0002] Resveratrol, a secondary metabolite of Vitis amurensis Rupr., has many beneficial effects on the human body, such as preventing cancer, protecting the cardiovascular system, antibacterial, and regulating blood lipids. The existing technology for extracting resveratrol mainly extracts grape plants or grape fruits as raw materials, but the raw materials of this extraction method are limited by seasons and growth cycles, and the continuity of the extraction work cannot be guaranteed. Therefore, developed the use of grape callus as a raw material to extract the resveratrol contained therein, although the limitations of the growing season and cycle have been overcome, the existing culture methods cultivated and used as raw materials for extracting resveratrol ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/04C07C37/68C07C39/21
CPCC07C37/68C12N5/04C07C39/21
Inventor 孙丹艾军秦洪艳许培磊赵滢刘迎雪王振兴杨义明范书田
Owner INST OF SPECIAL ANIMAL & PLANT SCI OF CAAS
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