Anoectochilus roxburghii hormone-free container culture method and culture medium thereof

A cultivation method and culture medium technology, applied in horticultural methods, botany equipment and methods, horticulture, etc., can solve problems such as slow growth of clematis, and achieve the effects of favorable root development, neat plants, and good growth and development

Active Publication Date: 2017-01-04
四川千草生物科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the cultivation and production, it was found that the growth of clematis is very slow, and the top will be capped at about 15cm, and the whole growth cycle is 3-5 months

Method used

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Examples

Experimental program
Comparison scheme
Effect test

specific Embodiment 1

[0028] (1) Use an 800mL orchid tissue culture bottle as a culture bottle, and hold 200mL of culture medium in the culture bottle;

[0029] (2) In step (1), 15 A. clematis tissue culture rooted seedlings with a height of 3 cm to 5 cm were planted in each culture bottle. The tissue culture temperature was 25±2°C, the light intensity was 2000 Lx, and the light time was 14 h / d, clematis can be harvested after 90 days of cultivation, and the plant height of clematis is an average of 15.9cm;

[0030] The 200mL culture medium composition comprises:

[0031] 3g / L peptone;

[0032] 100g / L potato juice;

[0033] 150g / L bean sprouts juice;

[0034] 0.5g / L activated carbon;

[0035] 30g / L of sucrose;

[0036] 8g / L agar strips;

[0037] The balance is MS medium.

specific Embodiment 2

[0039] (1) Use an 800mL orchid tissue culture bottle as a culture bottle, and hold 200mL of culture medium in the culture bottle;

[0040] (2) In step (1), 15 A. clematis tissue culture rooted seedlings with a height of 3 cm to 5 cm were planted in each culture bottle. The tissue culture temperature was 25±2°C, the light intensity was 2000 Lx, and the light time was 14 h / d, clematis can be harvested after 90 days of cultivation, and the plant height of clematis is an average of 16.2cm;

[0041] The 200mL culture medium composition comprises:

[0042] 3g / L peptone;

[0043] 130g / L potato juice;

[0044] 180g / L bean sprouts juice;

[0045] 0.5g / L activated carbon;

[0046] 30g / L of sucrose;

[0047] 8g / L agar strips;

[0048] The balance is MS medium.

specific Embodiment 3

[0050] (1) Use an 800mL orchid tissue culture bottle as a culture bottle, and hold 200mL of culture medium in the culture bottle;

[0051] (2) In step (1), 15 A. clematis tissue culture rooted seedlings with a height of 3 cm to 5 cm were planted in each culture bottle. The tissue culture temperature was 25±2°C, the light intensity was 2000 Lx, and the light time was 14 h / d, clematis can be harvested after 90 days of cultivation, and the plant height of clematis is an average of 16.5cm;

[0052] The 200mL culture medium composition comprises:

[0053] 3g / L peptone;

[0054] 150g / L potato juice;

[0055] 200g / L bean sprouts juice;

[0056] 0.5g / L activated carbon;

[0057] 30g / L of sucrose;

[0058] 8g / L agar strips;

[0059] The balance is MS medium.

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Abstract

The invention relates to an anoectochilus roxburghii hormone-free container culture method and a culture medium thereof, so as to solve the technical problems of anoectochilus roxburghii hormone-free container culture in the prior art. The culture method comprises the following steps of: (1) with a 800mL orchid tissue culture bottle as a culture bottle, putting 200mL of culture medium in the culture bottle; and (2) planting 15 anoectochilus roxburghii tissue culture rooting seedlings with height of 3-5cm in each tissue culture bottle in the step (1), wherein the tissue culture temperature is 25+ / 2 DEG C, the illumination intensity is 2,000Lx, and the illumination time is 14h / d; and harvesting anoectochilus roxburghii after 90 days of culture. Indoor container culture replaces field cultivation; after 90 days of culture, the average plant height reaches 16.5cm.

Description

technical field [0001] The invention relates to a hormone-free containerized culture method of A. aurea clematis and a culture medium thereof, and belongs to the technical field of hormone-free MS culture medium culture with organic matter added. Background technique [0002] Clematis Anoectochilus roxburghii (Wal1.) Lindl is a perennial herbaceous plant of the genus Orchidaceae. Clematis is mainly distributed in my country's Fujian, Taiwan, Guangdong, Guangxi, Hainan, Sichuan, Guizhou and Yunnan in hilly areas with an altitude of 300-1200m. The content of amino acids, anti-aging active substances and trace elements in clematis is higher than that of wild American ginseng, and it is a kind of Chinese herbal medicine that has been widely used. The whole plant can be used as medicine, which has the effects of anti-inflammatory and pain relief, nourishing yin and reducing fire, clearing away heat and detoxification. "Golden Grass" and "King of Medicine". In addition to its ...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 廖欣黄作喜李强罗崇玮
Owner 四川千草生物科技股份有限公司
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