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Method for enabling female aedes albopictus affected by Wolbach bacteria to be infertile

A technology of Wolbachia albopictus and Aedes albopictus, which is applied in animal husbandry and other fields, can solve the problems of great impact on host fitness, low efficiency, and prolonging the breeding time, so as to eliminate the threat of population replacement and improve production efficiency Effect

Inactive Publication Date: 2017-01-04
GUANGZHOU WOLBAKI BIOTECH CO LTD
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AI Technical Summary

Problems solved by technology

The first male and female isolates of mosquitoes were successfully constructed on Anopheles arabiensis. However, due to the instability of this mosquito strain and its great influence on the fitness of the host, it has been abandoned.
The second separation method is to add ivermectin to the blood meal. Female mosquitoes will die after sucking blood, and male mosquitoes will survive because they do not suck blood. However, this method has low estrogen removal efficiency and will prolong the feeding time (You must wait for the female mosquito to suck blood, usually 3-4 days)
Since there are no male and female isolates of Aedes albopictus at present, the remaining female mosquitoes after passing through the separator must be selected one by one before being released in the wild to eliminate the threat of population replacement. However, the efficiency of artificial female removal is low and cannot be applied in large-scale application

Method used

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  • Method for enabling female aedes albopictus affected by Wolbach bacteria to be infertile
  • Method for enabling female aedes albopictus affected by Wolbach bacteria to be infertile
  • Method for enabling female aedes albopictus affected by Wolbach bacteria to be infertile

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Embodiment 1

[0020] 1. Rapid identification of female and male pupae of Aedes albopictus includes the following steps:

[0021] (1) After the larvae of Aedes albopictus infected with Wolbachia have developed to the pupal stage, use a Pasteur pipette to pick out the pupae and place them on a petri dish, absorb the water around the pupae, and then observe the pupae under a dissecting microscope of the tail. Such as figure 1 , the short tail apparatus is the female pupa (left in the picture), and the long one is the male pupa (right in the picture).

[0022] 2. Sterility evaluation of female mosquitoes after radiation:

[0023] (1) After separating the female pupae under the microscope (the pupal age is 4-24 hours), place the female pupae in the middle of the radiation disk, and then use an X-ray radiation instrument (RS 2400) to irradiate (0, 23, 26, 28 , 32, 40Gy), about 50 female pupae were irradiated each time. After irradiation, the irradiated female pupae and the non-irradiated male...

Embodiment 2

[0040]1. Sterility evaluation of female mosquitoes after radiation:

[0041] (1) Large-scale rearing of Aedes albopictus larvae infected with Wolbachia to the pupal stage (4-40 hours), and then using a Fay-Morlan separator to preliminarily separate the female and male pupae. Take about 60,000-70,000 male pupae, mix them with 5,000 female pupae, then concentrate the pupae in a radiation cup, and use an X-ray radiation apparatus (Wolbaki ray apparatus) to irradiate (0, 40, 50 and 60Gy). After the irradiation, the male and female pupae were separated again using the separator, and the irradiated female pupae and the non-irradiated male pupae were placed in a clean cage at a ratio of about 1000:400.

[0042] (2) After all the pupae have emerged, provide 10% glucose solution to the adult mosquitoes. After 4 to 5 days, the female mosquitoes were given a blood meal. 48 hours after the blood meal, put it into the oviposition cup and collect the mosquito eggs for 48 hours. Then give...

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Abstract

The invention discloses a method for enabling female aedes albopictus affected by Wolbach bacteria to be infertile. The method comprises the steps that female aedes albopictus or male aedes albopictus containing female aedes albopictus is placed under X-ray for irradiation, the irradiation dose is larger than or equal to 28 Gy, the female aedes albopictus is made to be infertile, and the age of the female aedes albopictus is 4-40 h. X ray is adopted for performing irradiation in an aedes albopictus pupal period, so that the female aedes albopictus cannot generate offspring, and group replacement risk caused by accident release of the female aedes albopictus affected by the novel Wolbach bacteria is eliminated. Compared with a traditional artificial female removal method, the method can rapidly and effectively enable the female aedes albopictus to be infertile, the production efficiency is greatly improved, and the method is suitable for large-scale production and application.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for making female mosquitoes infected with Wolbachia (wPip) Aedes albopictus sterile. [0002] Background technique: [0003] Wolbachia (Wolbachia) is an intracellular Gram-negative commensal bacterium with maternal vertical inheritance, which is carried by about 40% of arthropods in nature. They can regulate host reproductive functions, such as inducing host cytoplasmic incompatibility (Cytoplasmic incompatibility, CI). CI refers to the incompatibility of the cytoplasm of the fertilized egg and the egg, resulting in abnormal development of the embryo and early death. CI is induced when Wolbachia-infected males mate with uninfected or infected females of different types. Therefore, a strategy based on the symbiotic bacteria to control pests and disease vectors, also called cytoplasmic incompatible technique (Incompatible insect technique, IIT), has been graduall...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/033
CPCA01K67/033
Inventor 张东京王晓华朱俭罗永平
Owner GUANGZHOU WOLBAKI BIOTECH CO LTD
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