Triterpenoid AntcinK and liver-protecting activity and application thereof

A 25r-antcink, triterpenoid technology, applied to the triterpenoid Antcin K and its hepatoprotective activity and application fields, can solve the problem that the pharmacological activity of Antrodia cinnamomea is not fully studied, the medicinal materials are scarce, and the fingerprint of the extraction method of Antrodia camphorata is not declared. problem, to achieve the effect of protecting the activity of abnormal lipid accumulation and significantly preventing and curing

Active Publication Date: 2017-01-04
PEKING UNIV
View PDF4 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Chinese patent CN101785793A discloses that Antrodia Antrodia Capsules (the contents of which are solid-cultured Antrodia Antrodia extracts) from Shansheng Biotechnology Co., Ltd. can alleviate chronic hepatitis induced by carbon tetrachloride. In this finding, no statement is made about the method of Antrodia Antrodia extraction and the capsules used. Fingerprint, also unknown main components
Due to the scarcit...

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Triterpenoid AntcinK and liver-protecting activity and application thereof
  • Triterpenoid AntcinK and liver-protecting activity and application thereof
  • Triterpenoid AntcinK and liver-protecting activity and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Embodiment 1, the separation and purification of triterpenoid Antcin K in Antrodia camphorata

[0034] 1. Experimental materials and methods.

[0035] All chemical reagents mentioned in this method were purchased from Beijing Chemical Plant.

[0036] First take 500 g of dried Antrodia camphorata fruiting body cultured on a plate and pulverize into coarse powder, divide into two batches, and use 6L 95% ethanol to reflux extract each batch for 6 times, each time for 3 hours; combine the extracts and rotary steam under reduced pressure to obtain about 200 g of total extract; After the total extract was suspended in 4 L of water, it was extracted 5 times with 4 L of petroleum ether successively, and the aqueous phase was extracted 5 times with ethyl acetate; the combined extract was evaporated to dryness to obtain about 132 g of the ethyl acetate layer.

[0037] About 130g of the extract from the ethyl acetate layer was separated through a silica gel column (200-300 mesh, Qi...

Embodiment 2

[0038] Embodiment 2, triterpenoid Antcin K in Antrodia camphorata to CCl 4 Protective effect of HepG2 cells induced by acute injury

[0039] 1. Experimental materials and methods.

[0040] HepG2 human liver cancer cells were purchased from the American Type Culture Collection (ATCC), and cells in logarithmic growth phase were used in the experiment. Carbon tetrachloride (Aladdin, Shanghai, China), DMEM medium (Zhongke Maichen, China), DMSO (dimethyl sulfoxide) (AppliChem), premium fetal bovine serum (PAN, South Africa), penicillin- Streptomycin cell culture double antibody (Zhongke Maichen, China), MTS (Promega, USA), silymarin (Huazhong Haiwei, Beijing).

[0041] SpectraMax Plus 384 light absorption microplate reader (Molecular Devices, USA), 3111 cell culture incubator (Thermo, USA), electronic analytical balance (Satorious, Germany).

[0042] CCl 4 Stock configuration: DMSO and CCl 4 Mix 1:1 (v / v), vortex to get 50% CCl 4 stock solution. Add stock solution to DMEM me...

Embodiment 3

[0055] Embodiment 3, Antcin K to CCl 4 Protective effect of acute liver injury caused by

[0056] 1. Experimental materials and methods.

[0057] 4-6 weeks old male ICR mice were purchased from the Experimental Animal Department of Peking University Health Science Center, given 12 hours of light and 12 hours of darkness, constant temperature, normal feed and water, and adapted to a sterile environment for 4 days before the experiment.

[0058] Carbon tetrachloride (Aladdin, Shanghai, China), silymarin (Huazhong Haiwei, Beijing, China), corn oil (Aladdin, Shanghai, China), sodium carboxymethylcellulose (Beijing Chemical Plant, Beijing, China) , deionized water (made by Milli-Q system), paraformaldehyde (Legend Bio, Beijing, China), Tranzol (full gold, Beijing), chloroform, ethanol, isopropanol (Beijing Chemical Plant, Beijing), RNA solution (Quanshijin, Beijing), RNase-free double distilled water, reverse transcription kit (Fermantas, USA), Sybr-green fluorescent dye method q...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a triterpenoid AntcinK and liver-protecting activity and application thereof. The triterpenoid AntcinK is extracted from wild, basswood-cultured and/or plate-cultured antrodia camphorate sporocarps, multiple activity screenings prove that the triterpenoid has the outstanding activity of protecting acute hepatitis liver damage caused by CCl4 and the activity of protecting abnormal lipid accumulation induced by alcohol. The compound has the remarkable preventing and treating effect on acute hepatitis, liver damage and alcoholic fatty liver and can be widely applied to liver protecting drugs and health-care products.

Description

technical field [0001] The invention relates to an extraction and separation method of antrodia antrodia triterpenoid Antcin K, and a new drug application of the compound as a liver-protecting drug, in particular to its application in protecting acute liver damage caused by acute hepatitis and protecting alcoholic fatty liver. Background technique [0002] Antrodia camphorata (Antrodia camphorata, Antrodia cinnamomea), also known as antrodia camphorata, camphor mushroom, camphor mushroom, camphor mushroom, blood ganoderma, camphor mushroom, red Antrodia camphorata, camphor fungus, red camphor sol, etc., is a kind of A rare fungus that parasitizes camphor trees unique to Taiwan, China. Antrodia cintrodia was first reported in 1990. At present, it is generally believed that Antrodia cintrodia belongs to the kingdom Fungi, Basidiomycota, Homobasidiomyeetes, Aphyllophorales, Polyporaeeae, and Thylobacteria Genus (Antrodia). It has been widely used as an anti-cancer, liver prot...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C07J9/00A61K31/575A61P1/16A23L33/105
Inventor 叶敏李紫薇乔雪匡易
Owner PEKING UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap