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Rare allelic variation of wheat grain weight gene TaGW2-6A and molecular marker and application thereof

A gene and grain weight technology, applied in the biological field, can solve the problems of uncertain genetic effects of QTLs and inability to accurately select target QTLs.

Active Publication Date: 2017-01-04
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are two main reasons for this phenomenon: first, the genetic effect of QTL is uncertain, and most QTL mapping results have not been tested by other genetic backgrounds; Hundreds of genes, so it is impossible to make accurate selection for the target QTL in breeding

Method used

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  • Rare allelic variation of wheat grain weight gene TaGW2-6A and molecular marker and application thereof
  • Rare allelic variation of wheat grain weight gene TaGW2-6A and molecular marker and application thereof
  • Rare allelic variation of wheat grain weight gene TaGW2-6A and molecular marker and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0060] Embodiment 1, the acquisition of molecular markers related to wheat grain weight

[0061] 1. Alignment of CDS sequences of TaGW2-6A gene

[0062] Yumai 8679 (YM8679) and Jing 411 (J411) are commonly used wheat varieties, but there are differences in grain weight between them. Therefore, the TaGW2-6A gene related to grain weight in the genomes of Yumai 8679 (YM8679) and Jing 411 (J411) was cloned, and the differences between the two in different varieties were compared. The CDS sequence of the TaGW2-6A gene is completely identical, which is sequence 1 in the sequence listing, and the sequence length is 1278 bp, encoding a protein TaGW2-6A with 425 amino acids, and the amino acid sequence of the protein is sequence 2 in the sequence listing.

[0063] Align the amino acid sequence of the RING domain of TaGW2-6A protein with the RING domain of other species, the results are as follows figure 1 shown.

[0064] figure 1 In the figure, the horizontal line is the RING domai...

Embodiment 2

[0136] Embodiment 2, application of grain weight-related molecular markers

[0137] 1. Obtaining the samples to be tested

[0138] Using Yumai 8679 as the female parent and Jing 411 as the male parent, the hybrid offspring F was obtained by crossing 1 After that, the derived F 2 generational segregation groups.

[0139] 2. PCR amplification of molecular markers related to grain weight

[0140] Extract Yumai 8679, Jing 411 and F 2 Genomic DNA of each individual plant in the first-generation segregation population was used as a template, and the primers TaGW2-6Atgw primers in Example 1 were used for PCR amplification to obtain PCR amplification products.

[0141] Check the size of the PCR product.

[0142] f 2 The PCR amplification products of 41 individual plants in the generation isolation population only contained a fragment of 222bp (1705-1926 nucleotides in sequence 3), and the TaGW2-6A gene promoter of these wheats lacked a 114bp DNA fragment. Genotype is defined as...

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Abstract

The invention discloses a rare allelic variation of a wheat grain weight gene TaGW2-6A and a molecular marker and an application thereof. The invention provides a method for identifying or assisting in identifying a grain weight and / or grain traits of a plant to be detected. The method comprises the following steps: detecting whether a promoter of a TaGW2-6A in a genome of the plant to be detected misses a 114bpDNA fragment, wherein the grain weight of the plant to be detected with the promoter of TaGW2-6A gene deleting the 114bpDNA fragment is greater than or candidate greater than the grain weight of the promoter of the TaGW2-6A not deleting the 114bpDNA fragment, or the grain shapes of the plant to be detected with the promoter of the TaGW2-6A gene deleting the 114bpDNA fragment is better than or candidate better than the grain shapes of the plant to be detected with the promoter of the TaGW2-6A gene not deleting the 114bpDNA. The allelic variation of the TaGW2-6A gene and the molecular marker TaGW2-6Atgw have great application potential in the wheat molecule design breeding.

Description

technical field [0001] The invention relates to a rare allelic variation of wheat grain weight gene TaGW2-6A and its molecular marker and application, belonging to the field of biotechnology. Background technique [0002] Wheat is the third largest food crop in my country, and continuous improvement of its production level is a strategic need to ensure my country's food security. With the increase of population and per capita consumption level, it is estimated that by 2030, my country's total annual demand for wheat will reach 170 million tons. Therefore, wheat high-yield breeding and yield traits genetics research are facing great challenges. Thousand-grain weight is one of the important yield components of wheat, which has been significantly improved in the genetic improvement of wheat varieties in my country. Zhuang Qiaosheng (2003) analyzed the wheat varieties cultivated in my country from 1949 to 2000, and found that the average thousand-grain weight of the varieties ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 倪中福孙其信翟会杰杜晓芬冯智宇宋艳娥彭惠茹姚颖垠李慧敏
Owner CHINA AGRI UNIV