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Slide or test paper array for detecting liquid calcium ions and preparation method and application method of slide or test paper array

A technology of ion detection and liquid calcium, which is applied in the field of analytical chemistry, can solve the problems of lack of high-throughput detection, test paper does not have hydrophobic properties, cross-contamination of reaction samples, etc., and achieve high detection accuracy, good selectivity, and intuitive detection. Effect

Inactive Publication Date: 2017-01-04
CENT SOUTH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the existing test paper detection methods, there is a shortcoming of lack of high-throughput detection; the test paper does not have hydrophobic properties, and it is easy to cause cross-contamination of reaction samples

Method used

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  • Slide or test paper array for detecting liquid calcium ions and preparation method and application method of slide or test paper array
  • Slide or test paper array for detecting liquid calcium ions and preparation method and application method of slide or test paper array

Examples

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Embodiment 1

[0029] The glass slide or test paper array used for liquid calcium ion detection in this embodiment is a glass slide or test paper impregnated with concentrated sulfuric acid, hexadecyltrimethoxysilane, and isopropanol, wherein according to mass percentage, concentrated sulfuric acid, cetyl The mass fractions of hexaalkyltrimethoxysilane and isopropanol are 0.1%, 3%, and 96.9% respectively; when in use, APS-CCA solution is added dropwise as a colorimetric probe, and the mass fraction of APS in the solution is 10%, The concentration of CCA was 10mmol / L to form a 5×10 hydrophobic colorimetric probe solution dot array.

[0030] The preparation method of the glass slide or test paper array used for liquid calcium ion detection in this embodiment is carried out according to the following steps:

[0031] (1) Preparation of solution A: according to the mass percentage, concentrated sulfuric acid: 0.1%, cetyltrimethoxysilane: 3%, isopropanol: 96.9% batching, each component is stirred ...

Embodiment 2

[0040] The glass slide or test paper array used for liquid calcium ion detection in this embodiment is a glass slide or test paper impregnated with concentrated sulfuric acid, hexadecyltrimethoxysilane, and isopropanol, wherein according to mass percentage, concentrated sulfuric acid, cetyl The mass fractions of hexaalkyltrimethoxysilane and isopropanol are 3%, 17%, and 80% respectively; when in use, APS-CCA solution is added dropwise as a colorimetric probe, and the mass fraction of APS in the solution is 0.1%, The concentration of CCA was 0.1mmol / L to form a 10×10 hydrophobic colorimetric probe solution dot array.

[0041] The preparation method of the glass slide or test paper array used for liquid calcium ion detection in this embodiment is carried out according to the following steps:

[0042] (1) Prepare solution A: according to the mass percentage, concentrated sulfuric acid: 3%, cetyltrimethoxysilane: 17%, isopropanol: 80%, and each component is stirred to obtain solut...

Embodiment 3

[0051] The glass slide or test paper array used for liquid calcium ion detection in this embodiment is a glass slide or test paper impregnated with concentrated sulfuric acid, hexadecyltrimethoxysilane, and isopropanol, wherein according to mass percentage, concentrated sulfuric acid, cetyl The mass fractions of hexaalkyltrimethoxysilane and isopropanol are 2%, 18%, and 80% respectively; when in use, APS-CCA solution is added dropwise as a colorimetric probe, and the mass fraction of APS in the solution is 20%, The concentration of CCA was 25mmol / L to form a 5×5 hydrophobic colorimetric probe solution dot array.

[0052] The preparation method of the glass slide or test paper array used for liquid calcium ion detection in this embodiment is carried out according to the following steps:

[0053] (1) Preparation solution A: according to mass percentage, concentrated sulfuric acid: 2%, hexadecyltrimethoxysilane: 18%, isopropanol: 80%, the sum of each component is 100% ingredients...

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Abstract

The invention belongs to the technical field of analytical chemistry, and specifically relates to a slide or test paper array for detecting liquid calcium ions and a preparation method and application method of the slide or test paper array. The slide or test paper array is slides or test paper soaked with concentrated sulfuric acid, hexadecyltrimethoxysilane and isopropanol. The mass percentage of concentrated sulfuric acid, hexadecyltrimethoxysilane and isopropanol is 0.1-3%, 3-18% and 80-96.9%. During use, an APS-CCA solution is added dropwise and taken as a colorimetric probe, wherein the mass percentage of APS in the solution is 0.1-20%, and the concentration of CCA is 0.1-25 mmol / L, and a lattice array of a colorimetric probe solution is formed. The slide or test paper array is fast, simple and visualized, is convenient to carry, and can be used for on-site detection. The array has high throughput, and can detect multiple samples at the same time. The array is excellent in hydrophobic performance, so that cross contamination between to-be-detected samples is greatly reduced. The array is simple and easily-controlled in preparation process, has excellent selectivity of calcium ions, and is high in detection accuracy.

Description

technical field [0001] The invention belongs to the technical field of analytical chemistry, and in particular relates to a glass slide or test paper array for liquid calcium ion detection and a preparation and use method. Background technique [0002] Calcium ion is an important ion with important physiological activities in the human body. It is closely related to many important physiological activities of the human body, such as participating in nerve conduction, muscle contraction, heart contraction, brain function, enzyme function and endocrine gland secretion. Hormones and other physiological functions; at the same time, calcium ions entering cells can act as important second messengers to trigger electrical signals and initiate intracellular events such as secretion, contraction, synaptic transmission and gene expression. In the earliest stages of life, calcium ions initiate the fertilization process by regulating the physiological activities of cells and directing ce...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78G01N21/64
CPCG01N21/643G01N21/78G01N2021/7756G01N2021/7759G01N2021/7786
Inventor 丁艳君凌江蔡继峰郭亚东扎拉嘎白乙拉
Owner CENT SOUTH UNIV
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