Analysis method of bioavailable phosphorus in algae and aquatic plant

An aquatic plant, effective phosphorus enzyme technology, applied in the direction of analysis by chemical reaction of materials, analysis of materials, material analysis by observing the effect on chemical indicators, etc.

Inactive Publication Date: 2017-01-04
CHINESE RES ACAD OF ENVIRONMENTAL SCI
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However, the combination of these two methods applied to algae and aquatic plants to analyze th...

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  • Analysis method of bioavailable phosphorus in algae and aquatic plant
  • Analysis method of bioavailable phosphorus in algae and aquatic plant
  • Analysis method of bioavailable phosphorus in algae and aquatic plant

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Embodiment 1

[0047] A method for analyzing biologically available phosphorus of algae and aquatic plants, comprising the following steps:

[0048] (1) Enzyme hydrolysis method

[0049] (1) Preparation of enzyme solution

[0050] The alkaline phosphatase (APase) solution configuration method that activity is 1U / mL is: get 3.33mg alkaline phosphatase (numbering is EC3.1.3.1, activity is 30U / mg) solid powder that buys from Sigma company, with 0.1 mol / L, Tris-HCl buffer solution with pH=9.0 was adjusted to 100 mL.

[0051] Activity is the alkaline phosphatase and phosphodiesterase (PDEase) combination solution configuration method of 0.02U / mL: get 10 mg of phosphodiesterase solid powder (numbering EC3.1.4.1, activity is 0.02U) purchased from Sigma company / mL), use 10mL alkaline phosphatase solution with an activity of 1U / mL to dissolve.

[0052] Activity is the alkaline phosphatase, phosphodiesterase and phytase (Phytase) combination solution configuration method of 0.06U / mL: get the crude...

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Abstract

The invention relates to an analysis method of bioavailable phosphorus in algae and aquatic plant. The analysis method includes the steps that the concrete phosphorus components in the lake algae and aquatic plant are analyzed through liquid-state 31P-NMR technology and an enzyme hydrolysis method and compared, it is found that the phosphorus components detected by the two methods are similar, wherein it is detected through the liquid-state 31P-NMR technology that the algae and aquatic plant contain 15 or more kinds of phosphorus components, it is found through the enzyme hydrolysis method that phytate phosphorus and an active monoester phosphorus component are high in hydrolysis capacity and account for 50.2% and 43.5% of the total hydrolysable organophosphorus respectively; various enzymes are added for culture to research the bioavailable phosphorus in the lake algae and aquatic plant, it is found that most monoester phosphorus and pyrophosphate in the organic phosphorus of the lake algae and aquatic plant are hydrolyzed into orthophosphate capable of being used directly by biont to different extents, sufficient nutrient substances are provided for algae breakout once more, and when the outside environment conditions are suitable, algae will break out circularly, which brings huge hidden danger to a lake ecosystem and drinking safety of the human.

Description

technical field [0001] The invention relates to an analysis method of biologically available phosphorus, in particular to the liquid state of biologically available phosphorus of algae and aquatic plants 31 P-NMR and enzymatic hydrolysis analysis methods. Background technique [0002] Organic phosphorus exists in lake water, sediments and aquatic plants, and it can be converted into biologically available phosphorus (that is, phosphorus that can be directly absorbed and utilized by organisms, such as orthophosphate, etc.) through a series of redox environments and enzymatic hydrolysis processes. Zhu [1] It is reported that the average content of organic phosphorus in sediment is 454mg / kg, and the concentration of organic phosphorus in aquatic plants and algae is 1.8 times and 12.9 times that of sediment, respectively. Although endogenous organic phosphorus in lakes plays an important role in the process of eutrophication, there are few reports on the composition and struct...

Claims

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Application Information

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IPC IPC(8): G01N21/78G01N24/08G01N1/28
CPCG01N21/78G01N1/28G01N24/082
Inventor 冯伟莹吴丰昌朱元荣宋凡浩白英臣
Owner CHINESE RES ACAD OF ENVIRONMENTAL SCI
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