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Method for establishing fingerprint spectrum to identify Phoebe zhennan based on GC-MS technology

A fingerprint and GC-MS technology, which is applied in the field of wood identification, can solve the problems of high error rate in identification of similar wood species, sluggish golden nan, and difficulty in distinguishing, so as to achieve accurate and reliable detection results, save experimental time, and high detection sensitivity Effect

Inactive Publication Date: 2017-01-04
SICHUAN AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, much to the embarrassment of law enforcement, consumer associations, and the timber industry, there are currently no standards for identifying Phoebe species
The blankness of the identification standard of Phoebe nanmu has led to the embarrassment that there are a large number of counterfeit Phoebe nanmu in the market, but the relevant agencies are unable to identify it. The main reason for the downturn in the current Jinshen market
In addition, there are still many problems in the identification of Zhennan wood species: because Zhennan and other tree species of the same genus and related genera have very high similarities in wood macroscopic, microstructural characteristics and material properties, only based on traditional wood identification It is difficult to distinguish them by methods, which leads to a large error rate in the identification of similar wood species. The inventors have received many cases where we have been asked to re-identify zhennan wood species due to misidentification by other institutions
Although there are currently a small number of relevant papers and patent reports on the identification of plants through chemical composition analysis, most of the above-mentioned technologies and methods cannot be directly used for wood species identification, and even several research papers related to wood species identification cannot be directly applied. The identification of Phoebe zhennan and other woods in the same genus and related genera, and the research results are still in the exploratory stage

Method used

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  • Method for establishing fingerprint spectrum to identify Phoebe zhennan based on GC-MS technology
  • Method for establishing fingerprint spectrum to identify Phoebe zhennan based on GC-MS technology
  • Method for establishing fingerprint spectrum to identify Phoebe zhennan based on GC-MS technology

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0086] Mianyang zhennan was naturally air-dried to a moisture content of 11.5%. After crushing, take 4±0.1g of a sample that passed through a 30-mesh screen and put it in an extraction bottle. Insert the solid-phase microextraction handle into the bottle and extend the extraction head. Keep it warm for 40 minutes, return the extraction head, pull out the SPME handle, and obtain its essence sample. The sample was analyzed by GC-MS, and the analysis time was 3 minutes, and the total ion chromatogram of Zhennan Mianyang was obtained. The relative content of each component was calculated by the peak area normalization method, and the corresponding characteristic peaks were found. And the relative peak area data of the sample and Zhennan control sample were calculated according to the formula (1).

[0087] Such as Figure 20 As shown, comparing the total ion chromatogram obtained in Example 1 with the mass spectrum of the characteristic compound of Zhennan control sample, it can ...

Embodiment 2

[0089] Naturally air-dry Pengzhou Zhennan to a moisture content of 11.2%. After crushing, take 4±0.1g of a sample that passes through a 30-mesh sieve and put it in an extraction bottle. Insert the solid-phase microextraction handle into the bottle and extend the extraction head. Keep it warm for 40 minutes, return the extraction head, pull out the SPME handle, and obtain its essence sample. The sample was analyzed by GC-MS, and the analysis time was 3 minutes, and the total ion chromatogram of Zhennan Pengzhou was obtained. The relative content of each component was calculated by the peak area normalization method, and the corresponding characteristic peaks were found. And the relative peak area data of the sample and the control sample were calculated according to the formula (1).

[0090] Such as Figure 21 As shown, comparing the total ion chromatogram obtained in Example 2 with the mass spectrogram of the Zhennan control sample characteristic compound, it can be seen tha...

Embodiment 3

[0092] Naturally air-dry Xinjin Zhennan to a moisture content of 10.5%. After crushing, take 4±0.1g of a sample that passes through a 30-mesh sieve and put it in an extraction bottle. Insert the solid-phase microextraction handle into the bottle and extend the extraction head. Keep it warm for 40 minutes, return the extraction head, pull out the SPME handle, and obtain its essence sample. The sample was analyzed by GC-MS, and the analysis time was 3 minutes, and the total ion chromatogram of Xinjin Zhennan was obtained. The relative content of each component was calculated by the peak area normalization method, and the corresponding characteristic peaks were found. And the relative peak area data of the sample and the control sample were calculated according to the formula (1).

[0093] Such as Figure 22 As shown, comparing the total ion current diagram obtained in Example 3 with the mass spectrogram of Zhennan control sample characteristic compound, it can be seen that all...

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Abstract

The invention discloses a method for establishing a fingerprint spectrum to identify Phoebe zhennan based on the GC-MS technology. The method comprises the steps that firstly, essential gas or essential oil of a sample to be detected is extracted; the essential gas is subjected to GC-MS analysis, or the essential oil is diluted with diethyl ether and then subjected to GC-MS analysis, and a total ion current map is obtained; the relative content of all components is calculated through a peak area normalization method under the condition that chromatographic conditions and integral parameters are identical, corresponding characteristic peaks are found, the relative peak area data of the sample to be detected and a Phoebe zhennan control sample feature compound is input into a formula to calculate the correlation coefficient of the sample to be detected and the Phoebe zhennan control sample feature compound; finally, whether the sample to be detected is the Phoebe zhennan or not is judged according to Phoebe zhennan wood species identification indexes. The Phoebe zhennan fingerprint spectrum is established through the GC-MS technology to distinguish the Phoebe zhennan and wood species prone to be mixed, detecting efficiency is high, detecting sensitivity is high, loss of objects to be detected is small, and the detecting result is scientific, objective, accurate and reliable.

Description

technical field [0001] The application belongs to the technical field of wood identification, and in particular relates to a method for identifying Phoebe zhennan by establishing fingerprints based on GC-MS technology. Background technique [0002] Since 2008, golden nanmu has been widely concerned and enthusiastically sought after, and its price has been soaring all the way, especially after 2010, the growth rate was extremely fast, and in 2013, the price was close to that of Hainan huanghuali, the most valuable wood. However, the current identification and identification of golden nanmu species in the industry is rather confusing. According to GB / T16734-1997 "Names of Main Timbers in China", Phoebe refers to 8 species of Phoebe Nees in the family Lauraceae, but the industry believes that the most authentic golden nanmu refers to Phoebe zhennan ) tree species, its market price is much higher than that of other Phoebe tree species, and Zhennan is a second-class protected pla...

Claims

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Application Information

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IPC IPC(8): G01N30/02
CPCG01N30/02
Inventor 宁莉萍丁文张莉时浩熊燕安荣苗王天石张启文张帅刘江陈颐萱陈全
Owner SICHUAN AGRI UNIV
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