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Gleditsia delavayi total saponins extracting and purifying method

A technology for total saponins and acacia, which is applied in the field of extraction and purification of total saponins from Acacia yunnanensis, can solve the problems of high industrial production cost, reduced total saponin purity, large single solvent consumption, etc., achieves complete extraction, realizes pollution-free discharge, and shortens extraction time. effect of time

Inactive Publication Date: 2017-01-11
四川小叶本草生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The extraction rate of the water extraction method is relatively high, but some monosaccharides, oligosaccharides, amino acids, proteins, mucus, etc. are often extracted, resulting in a decrease in the purity of the total saponins in the extract
The extraction rate of the organic solvent extraction method is relatively high, the consumption of a single solvent is large, and the cost of industrial production is high
In short, there are still shortcomings such as low cost or high cost and poor operability in order to achieve large-scale production.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1: Dried acacia yunnanensis in an oven at 60°C, peeled, and crushed to 10 meshes, took 20Kg of fruit shell powder and put it into a pressurized solvent extractor, added 200L of distilled water, at a temperature of 80°C, and a pressure of 2.5 Kg / cm 2 Extract under pressure for 30 min, extract 3 times, combine extracts and centrifuge. The extract was cooled to room temperature, and 1% Rhodococcus erythropolis ( Rhodococcusery thropolis ), cultured at 35°C for 5 days, centrifuged, and the supernatant was taken. Use membranes with molecular weight cut-offs of 15000 and 5000Da for separation, and membrane filtration working pressure: 0.1~0.2Mpa. After the permeate passed through a 2000Da membrane, the extract was concentrated and then spray-dried to obtain 8.2Kg of total saponin powder from Dian saponins. The extraction rate of total saponins was 32.8%, and the purity of total saponins was 80.2%.

Embodiment 2

[0021] Example 2: Dian saponica shells were dried in an oven at 60°C, peeled, and crushed to 20 meshes. Take 20Kg of fruit shell powder and put it into a pressurized solvent extractor, add 100L of distilled water, and set the temperature at 100°C and the pressure at 4.0 Kg / cm 2 Extract under pressure for 30 min, extract 4 times, combine extracts and centrifuge. The extract was cooled to room temperature, and 3% Rhodococcus erythropolis ( Rhodococcusery thropolis ), cultured at 20°C for 8 days, centrifuged, and the supernatant was taken. Use membranes with molecular weight cut-offs of 15000 and 5000Da for separation, and membrane filtration working pressure: 0.1~0.2Mpa. After the permeated liquid passed through a 2000Da membrane, the extract was concentrated and then spray-dried to obtain 8.5Kg of total saponins powder from Acacia saponins. The extraction rate of total saponins was 35.0%, and the purity of total saponins was 82.3%.

Embodiment 3

[0022] Example 3, Dian saponica shells were dried in an oven at 60°C, peeled, and crushed to 10 meshes. Take 20Kg of fruit shell powder and put it into a pressurized solvent extractor, add 160L of distilled water, and heat at a temperature of 100°C and a pressure of 3.5 Kg / cm 2 Extract under pressure for 45 minutes, extract 6 times, combine the extracts and centrifuge. The extract was cooled to room temperature, and 7% Rhodococcus erythropolis ( Rhodococcusery thropolis ), cultured at 20°C for 10 days, centrifuged, and the supernatant was taken. Use membranes with molecular weight cut-offs of 15000 and 5000Da for separation, and membrane filtration working pressure: 0.1~0.2Mpa. After the permeate passed through a 2000Da membrane, the extract was concentrated and then spray-dried to obtain 8.3Kg of total saponins powder from Acacia saponins. The extraction rate of total saponins was 34.6%, and the purity of total saponins was 81.3%.

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Abstract

The invention provides a gleditsia delavayi total saponins extracting and purifying method. The method takes gleditsia delavayi shells as a raw material, and the method comprises the following steps: 1) the dried gleditsia delavayi shells are crushed to 10-20 meshes, then the powder is put in a pressurization solvent extraction apparatus, 1-10 times of distilled water is added, the pressurization solvent extractor is sealed, the material is pressurized and extracted for 40 min at the temperature of 80-110 DEG C and the pressure of 2.5-4.0 Kg / cm<2> for 3 times, solid-liquid separating is carried out, and the extracts are merged; 2) the above extracts are cooled to room temperature, 1-7% of Rhodococcus erythropolis is added and cultured for 2-10 days at the temperature of 20-35 DEG C, and a liquid supernatant is taken; 3) the membranes with the molecular weight cut-off of 15000, 5000, and 2000Da are respectively used for separating, wherein the membrane filtration work pressure is 0.1-0.2 MPa; 4) the extract through the 2000Da membrane is concentrated; and 5) a concentrate is subjected to spray drying to obtain the gleditsia delavayi total saponins powder.

Description

technical field [0001] The invention relates to the extraction of obsolete effective components of plants, in particular to a method for extracting and purifying total saponins from Acacia saponins. Background technique [0002] Dian saponica ( Gleditsia japonica var. delavayi ) belongs to the hematoxylin family Acacia genus. There are about 15 kinds of honey locusts in the world, and they are scattered in Central Asia, Southeast Asia and North and South America. There are 6 species and 2 varieties produced in China, which are widely distributed in the north and south provinces, mainly in the provinces of the Yangtze River Basin. There are 2 species in Yunnan, one is a variant. The polysaccharide plant gum obtained by separating and extracting the endosperm of acacia seeds is called acacia bean gum. The main component is galactomannan. Its properties are similar to guar gum, and it has good stability, thickening and hydration, and low concentration. Sometimes it has a hi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/483A61K8/97
Inventor 不公告发明人
Owner 四川小叶本草生物科技有限公司
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