Preparation method of rice dwarf virus antibody

Abstract

The invention belongs to the technical field of virus antibody preparation, and particularly relates to a preparation method of a rice dwarf virus antibody. The invention provides the preparation method of the rice dwarf virus antibody, which solves the problem that macromolecular target proteins are generally not easy to induce. The preparation method comprises the following steps: (1) inoculating a positive strain in two tubes of LB fluid mediums containing penbritin and chloramphenicol according to a ratio of 1 to 100, shaking for 3 to 4 h, and performing induced culture for 4 to 5 h, wherein IPTG is not added in another test tube for contrast; taking bacterial fluid, collecting thalli, and putting into Coomassie brilliant blue staining fluid after the thalli are subjected to gel electrophoresis separation; and (2) inducing 100 ml of the bacterial fluid, collecting thalli, performing gel electrophoresis on target proteins, performing KCL pre-cooling on gel subjected to electrophoresis to obtain a milk white protein band, cutting and recycling the target protein band with a blade, adding the freund's adjuvant, grinding to obtain emulsified proteins, and storing the emulsified proteins for later use.

Description

technical field [0001] The invention belongs to the technical field of virus antibody preparation, and in particular relates to a preparation method of rice dwarf virus antibody. Background technique [0002] Southern rice black-streaked dwarf disease was first discovered in Yangjiang City, Guangdong Province in 2001, and gradually spread to neighboring provinces in the following years. In 2009, a large-scale outbreak occurred in 9 provinces in southern China, and the affected area was about 300,000 hm2. In the same year, the disease commonly occurred in 19 provinces in northern Vietnam. In 2010, more than 1.3 million hectares of rice were damaged in 13 provinces in southern China, and about 60,000 hectares of rice were damaged in 29 provinces in central and northern Vietnam. In 2010, the disease was also found in Kyushu, Japan. In addition, the disease can not only harm rice, but also maize. In 2009, the disease was also detected in maize in Shandong, Guangdong, Guangxi a...

AI Technical Summary

Problems solved by technology

[0004] The present invention aims at the above problems and provides a method for preparing rice dwarf virus antibody that solves the problem that macromolecular target proteins are not easy to induce.