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Preparation method of polypyrrole-polydopamine-based electrochemical immunosensor

An immunosensor and polydopamine technology, which is applied in the fields of polymer material science and electrochemical bioanalysis, can solve the problems of functional modification and preparation difficulties, poor mechanical properties of polypyrrole, and lack of adhesion, etc. Difficulty in processing, low cost, and the effect of improving conductivity

Inactive Publication Date: 2017-01-11
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, polypyrrole is a black precipitate that is insoluble in water and most organic solvents, making its later functional modification and preparation very difficult
At the same time, the poor mechanical properties of polypyrrole and almost no adhesiveness have seriously hindered its industrial application.

Method used

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  • Preparation method of polypyrrole-polydopamine-based electrochemical immunosensor
  • Preparation method of polypyrrole-polydopamine-based electrochemical immunosensor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Step 1: Weigh 0.1g Py and 0.282g DA and dissolve in 40mL buffer, stir in ice bath, then add 5mL 1.0mg·mL -1 The APS solution, the molar ratio of Py and DA is 1:1, through N 2 Oxygen was removed for 15 minutes, and stirred for 12 hours under an ice-water bath. After the reaction was completed, the product was centrifuged and washed with deionized water until the supernatant was colorless. The resulting precipitate was dispersed in deionized water again, and the product PPy-PDA was obtained by freeze-drying;

[0022] Step 2: Weigh 0.005g PPy-PDA, add it to 6mL water, ultrasonically disperse for 1h, use a micrometer to absorb 10μL PPy-PDA aqueous solution and drop-coat it on the surface of the gold electrode, and let it dry naturally to obtain a PPy-PDA / Au electrode;

[0023] Step 3: Immerse the PPy-PDA / Au electrode to 0.5mg·mL -1 Au / PPy-PDA / Au electrode was obtained by rinsing with deionized water in the chloroauric acid solution for 12h. Immerse the Au / PPy-PDA / Au elec...

Embodiment 2

[0025] Step 1: Weigh 0.1g Py and 0.565g DA and dissolve in 40mL buffer, stir in ice bath, then add 5mL 1.0mg·mL -1 The APS solution, the molar ratio of Py and DA is 1:2, through N 2 Oxygen was removed for 15 minutes, and stirred for 12 hours under an ice-water bath. After the reaction was completed, the product was centrifuged and washed with deionized water until the supernatant was colorless. The resulting precipitate was dispersed in deionized water again, and the product PPy-PDA was obtained by freeze-drying;

[0026] Step 2: Weigh 0.005g PPy-PDA, add it to 6mL water, ultrasonically disperse for 1h, use a micrometer to absorb 10μL PPy-PDA aqueous solution and drop-coat it on the surface of the gold electrode, and let it dry naturally to obtain a PPy-PDA / Au electrode;

[0027] Step 3: Immerse the PPy-PDA / Au electrode to 0.5mg·mL -1 Au / PPy-PDA / Au electrode was obtained by rinsing with deionized water in the chloroauric acid solution for 12h. Immerse the Au / PPy-PDA / Au elec...

Embodiment 3

[0029] Step 1: Weigh 0.1g Py and 1.41g DA and dissolve in 40mL buffer, stir in ice bath, then add 5mL 1.0mg·mL -1 The APS solution, the molar ratio of Py and DA is 1:5, through N 2 Oxygen was removed for 15 minutes, and stirred for 12 hours under an ice-water bath. After the reaction was completed, the product was centrifuged and washed with deionized water until the supernatant was colorless. The resulting precipitate was dispersed in deionized water again, and the product PPy-PDA was obtained by freeze-drying;

[0030] Step 2: Weigh 0.005g PPy-PDA, add it to 6mL water, ultrasonically disperse for 1h, use a micrometer to absorb 10μL PPy-PDA aqueous solution and drop-coat it on the surface of the gold electrode, and let it dry naturally to obtain a PPy-PDA / Au electrode;

[0031] Step 3: Immerse the PPy-PDA / Au electrode to 0.5mg·mL -1 Au / PPy-PDA / Au electrode was obtained by rinsing with deionized water in the chloroauric acid solution for 12h. Immerse the Au / PPy-PDA / Au elect...

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Abstract

The invention discloses a preparation method of a polypyrrole-polydopamine-based electrochemical immunosensor, relating to the technical fields of high-polymer material science, electrochemical biological analysis and the like. The dopamine is introduced to improve the defect of poor water solubility in the polypyrrole, and the polydopamine with favorable adhesion property is utilized to enhance the interaction between the polypyrrole and the substrate. In addition, the weak reduction action of the polydopamine is utilized to perform in-situ reduction on chloroauric acid on the substrate surface to obtain gold nanoparticles, and the interaction between the metal particles and the antibody is utilized to adsorb the antibody, thereby obtaining the immunosensor capable of specifically adsorbing the corresponding antigen. By combining the conductive polypyrrole, nano technique and immunoreaction, the method has the advantages of simple preparation process, mild conditions and low cost. The obtained immunosensor has favorable conductivity, can selectively adsorb the corresponding antigen, is applicable to different antibodies and antigens, and has universality.

Description

technical field [0001] The invention relates to scientific and technological fields such as polymer material science and electrochemical bioanalysis, in particular to a preparation method of an immunosensor based on polypyrrole-polydopamine Background technique [0002] The mechanism of forming bioactive bodies through antigen-antibody binding reaction is very complex, and antigen-antibody binding reaction plays an important role in the prognosis and treatment of human diseases. Immunosensors are sensors that selectively recognize and detect antigens based on antigen-antibody binding reactions. Since there is a one-to-one correspondence between the combination of antigen and corresponding antibody, the electrochemical immunosensor based on antigen-antibody has high selectivity, sensitivity and good stability. However, the atomic-level antigen-antibody interaction usually requires X-ray light scattering technology or nuclear magnetic resonance technology to detect, and the p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327
CPCG01N27/3278
Inventor 罗静黄婧许升刘晓亚
Owner JIANGNAN UNIV
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