Method for extracting flavone from herba violae

A technology of Viola ditin and flavonoids, which is applied in separation methods, chemical instruments and methods, solvent extraction, etc., can solve the problems of polluting the environment, wasting reagents, wasting time, etc., and achieves the reduction of impurity content, fast extraction speed, and shortened time Effect

Inactive Publication Date: 2017-01-25
DAXINGANLING LINGOBERRY BOREAL BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, in the prior art, Viola dicin is used as raw material, and the methods of extracting flavonoids are decoction, reflux and ultrasonic methods. The efficiency of macroporous adsorption resin extraction is low, the reproducibility is not good, waste of time, waste of reagents and pollution Environment etc.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] (1) Wash Viola radix, dry, crush through a 40-mesh sieve, and weigh 100g;

[0022] (2) Under room temperature conditions, immerse the pulverized viola in 60% ethanol (making the volume ratio of material to ethanol 1:5) and soak for 30 minutes;

[0023] (3) Extract the Viola dicin mixture with microwave at 200W, 30°C for 5min;

[0024] (4) putting the above-mentioned extraction mixed solution into the extraction tank of the supercritical carbon dioxide extraction system;

[0025] (5) Supercritical carbon dioxide with a pressure of 25Mpa and a temperature of 35°C enters the extraction tank from the bottom and extracts for 1 hour;

[0026] (6) In a separation tank with a pressure of 1Mpa and a temperature of 20°C, the flavonoids are separated into open plastic bottles;

[0027] (7) The obtained flavonoids are yellow crystalline powder, and the content of total flavonoid glycosides is 95.15% as detected by liquid chromatography.

Embodiment 2

[0029] (1) Wash Viola radix, dry, crush through a 50-mesh sieve, and weigh 100g;

[0030] (2) Under room temperature conditions, immerse the pulverized viola in 70% ethanol (making the volume ratio of material to methanol 1:10) and soak for 45 minutes;

[0031] (3) Extract the Viola dicin mixture at 300W, 35°C, and microwave for 15 minutes;

[0032] (4) putting the above-mentioned extraction mixed solution into the extraction tank of the supercritical carbon dioxide extraction system;

[0033] (5) Supercritical carbon dioxide with a pressure of 32Mpa and a temperature of 48°C enters the extraction tank from the bottom and extracts for 1.5 hours;

[0034] (6) In a separation tank with a pressure of 1.5Mpa and a temperature of 22°C, the flavonoids are separated into open plastic bottles;

[0035] (7) The obtained flavonoids are yellow crystalline powder, and the content of total flavonoid glycosides is 95.94% as detected by liquid chromatography.

Embodiment 3

[0037] (1) Wash the Viola chinensis, dry, crush and pass through a 60-mesh sieve, and weigh 100g;

[0038] (2) Under room temperature conditions, immerse the pulverized viola in 80% ethanol (make the volume ratio of material to ethyl acetate 1:15), and soak for 60 minutes;

[0039] (3) Extract the Viola dicin mixture with microwave at 400W, 40°C for 20min;

[0040] (4) putting the above-mentioned extraction mixed solution into the extraction tank of the supercritical carbon dioxide extraction system;

[0041] (5) Supercritical carbon dioxide with a pressure of 45Mpa and a temperature of 60°C enters the extraction tank from the bottom and extracts for 1.5 hours;

[0042] (6) In a separation tank with a pressure of 2Mpa and a temperature of 25°C, the flavonoids are separated into open plastic bottles;

[0043] (7) The obtained flavonoids are yellow crystalline powder, and the content of total flavonoid glycosides is 96.38% as detected by liquid chromatography.

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Abstract

The invention belongs to the field of natural organic chemistry, and relates to a new method for extracting flavonoids from Viola radix. Vaporize quickly, burst the cell wall, and release the active ingredients as soon as possible; 2. Use supercritical carbon dioxide technology to purify Viola, so that the purity of flavonoids can reach more than 90%. Compared with the traditional process, the present invention has the advantages of rapidity, high efficiency, safety, energy saving, etc., while maintaining a high extraction rate, it greatly shortens the time used in the extraction process, and reduces the content of impurities in the extraction liquid. The invention has abundant sources of raw materials, reduces the production cost and is suitable for industrialized production.

Description

technical field [0001] The invention belongs to the field of natural organic chemistry, and relates to a method for extracting flavonoids from Viola chinensis. Background technique [0002] Viola yedoensis Makino (Viola yedoensis Makino) is a plant of the Viola family Viola genus, a perennial herb, also known as Viola yedoensis Makino, Viola yedoensis Makino, Arrowhead Grass, Makino, Jiaozi, Lone Tiger, Sword Grass, Plow Grass, Free Ears Grass, Jinqiandao, Xiaojiaozihua, the dried whole grass is used in traditional Chinese medicine, which has the effects of clearing away heat and detoxification, cooling blood and reducing swelling. Zihua Diding is mainly produced in Jiangsu, Zhejiang, Anhui and other places. [0003] The chemical components of Viola didin mainly include flavonoids, coumarins, organic acids, sugars, amino acids, proteins, saponins, phytosterols and tannins, etc. Among them, the active ingredient flavonoids can improve cardiovascular and cerebrovascular circu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/86A61P9/10A61P25/28B01D11/02B01D11/04
CPCA61K36/86A61K2236/333A61K2236/37A61K2236/39B01D11/02B01D11/0288B01D11/0403
Inventor 姚德坤刘玲
Owner DAXINGANLING LINGOBERRY BOREAL BIOTECH CO LTD
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