Method for extracting flavone from herba violae
A technology of Viola ditin and flavonoids, which is applied in separation methods, chemical instruments and methods, solvent extraction, etc., can solve the problems of polluting the environment, wasting reagents, wasting time, etc., and achieves the reduction of impurity content, fast extraction speed, and shortened time Effect
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Embodiment 1
[0021] (1) Wash Viola radix, dry, crush through a 40-mesh sieve, and weigh 100g;
[0022] (2) Under room temperature conditions, immerse the pulverized viola in 60% ethanol (making the volume ratio of material to ethanol 1:5) and soak for 30 minutes;
[0023] (3) Extract the Viola dicin mixture with microwave at 200W, 30°C for 5min;
[0024] (4) putting the above-mentioned extraction mixed solution into the extraction tank of the supercritical carbon dioxide extraction system;
[0025] (5) Supercritical carbon dioxide with a pressure of 25Mpa and a temperature of 35°C enters the extraction tank from the bottom and extracts for 1 hour;
[0026] (6) In a separation tank with a pressure of 1Mpa and a temperature of 20°C, the flavonoids are separated into open plastic bottles;
[0027] (7) The obtained flavonoids are yellow crystalline powder, and the content of total flavonoid glycosides is 95.15% as detected by liquid chromatography.
Embodiment 2
[0029] (1) Wash Viola radix, dry, crush through a 50-mesh sieve, and weigh 100g;
[0030] (2) Under room temperature conditions, immerse the pulverized viola in 70% ethanol (making the volume ratio of material to methanol 1:10) and soak for 45 minutes;
[0031] (3) Extract the Viola dicin mixture at 300W, 35°C, and microwave for 15 minutes;
[0032] (4) putting the above-mentioned extraction mixed solution into the extraction tank of the supercritical carbon dioxide extraction system;
[0033] (5) Supercritical carbon dioxide with a pressure of 32Mpa and a temperature of 48°C enters the extraction tank from the bottom and extracts for 1.5 hours;
[0034] (6) In a separation tank with a pressure of 1.5Mpa and a temperature of 22°C, the flavonoids are separated into open plastic bottles;
[0035] (7) The obtained flavonoids are yellow crystalline powder, and the content of total flavonoid glycosides is 95.94% as detected by liquid chromatography.
Embodiment 3
[0037] (1) Wash the Viola chinensis, dry, crush and pass through a 60-mesh sieve, and weigh 100g;
[0038] (2) Under room temperature conditions, immerse the pulverized viola in 80% ethanol (make the volume ratio of material to ethyl acetate 1:15), and soak for 60 minutes;
[0039] (3) Extract the Viola dicin mixture with microwave at 400W, 40°C for 20min;
[0040] (4) putting the above-mentioned extraction mixed solution into the extraction tank of the supercritical carbon dioxide extraction system;
[0041] (5) Supercritical carbon dioxide with a pressure of 45Mpa and a temperature of 60°C enters the extraction tank from the bottom and extracts for 1.5 hours;
[0042] (6) In a separation tank with a pressure of 2Mpa and a temperature of 25°C, the flavonoids are separated into open plastic bottles;
[0043] (7) The obtained flavonoids are yellow crystalline powder, and the content of total flavonoid glycosides is 96.38% as detected by liquid chromatography.
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