Manufacture and detection method of lucid ganoderma liquid strains
A technology of liquid strains and production methods, applied in the direction of microorganism-based methods, microorganism measurement/inspection, biochemical equipment and methods, etc., which can solve the problems of low content of biologically active substances, obstacles to mass production of Ganoderma lucidum, and restrictions on the industrialization of Ganoderma lucidum, etc. problem, to achieve the effect of strong hyphae permeability, labor saving, and fast feeding
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Embodiment 1
[0033] According to the mother seed purification medium described in formula A, the ganoderma lucidum stock is purified, the liquid medium described in formula C is used to cultivate the purified stock on a shaker, and the liquid strain of the previous step is cultured in a fermenter according to formula F to obtain Ganoderma lucidum liquid strain can be used after passing the test.
Embodiment 2
[0035] The parent species purification medium of Example 1 was replaced with formula B, the liquid medium was replaced with formula D, and the fermenter culture medium was replaced with formula G, and other conditions remained unchanged.
Embodiment 3
[0037] The parent species purification medium of Example 1 was replaced with formula B, the liquid medium was replaced with formula E, and other conditions remained unchanged.
[0038] Examples 1-3 only need 5-7 days on average to prepare ganoderma lucidum liquid strains with consistent bacterial age and strong permeability. Average shortened by 15-20 days. When adopting the liquid bacterial strain prepared by the present invention as the mother seed or the original seed to enlarge and cultivate the original seed or the cultivar, it is much faster than adopting the solid bacterial strain. Under the same control and other conditions, the general liquid strain matures 10-20d earlier than the solid strain. In addition, due to the fluidity of the liquid strains, each mycelial ball and mycelial fragments can spread to different parts to germinate. There are many development points, and the inner and outer sides grow together. The mycelia can germinate in 6~12 hours, and can be ful...
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