Manufacture and detection method of lucid ganoderma liquid strains

A technology of liquid strains and production methods, applied in the direction of microorganism-based methods, microorganism measurement/inspection, biochemical equipment and methods, etc., which can solve the problems of low content of biologically active substances, obstacles to mass production of Ganoderma lucidum, and restrictions on the industrialization of Ganoderma lucidum, etc. problem, to achieve the effect of strong hyphae permeability, labor saving, and fast feeding

Pending Publication Date: 2017-01-25
孝感市应用技术研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, Ganoderma lucidum has been planted in artificial greenhouses. The fruiting bodies (or sclerotia) produced by traditional field collection or artificial cultivation are limited by the scarcity of wild resources, long fruiting body cultivation and production cycle (4-5 months), and p

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] According to the mother seed purification medium described in formula A, the ganoderma lucidum stock is purified, the liquid medium described in formula C is used to cultivate the purified stock on a shaker, and the liquid strain of the previous step is cultured in a fermenter according to formula F to obtain Ganoderma lucidum liquid strain can be used after passing the test.

Embodiment 2

[0035] The parent species purification medium of Example 1 was replaced with formula B, the liquid medium was replaced with formula D, and the fermenter culture medium was replaced with formula G, and other conditions remained unchanged.

Embodiment 3

[0037] The parent species purification medium of Example 1 was replaced with formula B, the liquid medium was replaced with formula E, and other conditions remained unchanged.

[0038] Examples 1-3 only need 5-7 days on average to prepare ganoderma lucidum liquid strains with consistent bacterial age and strong permeability. Average shortened by 15-20 days. When adopting the liquid bacterial strain prepared by the present invention as the mother seed or the original seed to enlarge and cultivate the original seed or the cultivar, it is much faster than adopting the solid bacterial strain. Under the same control and other conditions, the general liquid strain matures 10-20d earlier than the solid strain. In addition, due to the fluidity of the liquid strains, each mycelial ball and mycelial fragments can spread to different parts to germinate. There are many development points, and the inner and outer sides grow together. The mycelia can germinate in 6~12 hours, and can be ful...

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PUM

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Abstract

The invention relates to a manufacture and a detection method of lucid ganoderma liquid strains. The method comprises the following steps: firstly, purifying a lucid ganoderma mother strain in a mother strain purification culture medium, inoculating the purified mother strain into a shake flask to be cultured in a liquid culture medium, taking out the cultured liquid culture medium, and inoculating the cultured liquid culture medium into a fermentation tank for continuous culture, and using the obtained liquid strain after the obtained liquid strain is qualified through detection. The mother strain purification culture medium, the liquid culture medium and a fermentation tank culture medium all have various formulas and can be flexibly selected according to situations. The liquid strain prepared from the method has the characteristics of consistent cell age, high permeability, short production period, early sprouting and high material absorbing speed.

Description

technical field [0001] The invention relates to the technical field of edible fungus cultivation, in particular to a preparation and detection method of ganoderma lucidum liquid strain. Background technique [0002] Ganoderma lucidum, commonly known as Ganoderma lucidum, is the fruiting body of Ganoderma lucidum, a fungus of Polyporaceae. Ganoderma lucidum has the effects of invigorating qi and calming the nerves, relieving cough and relieving asthma. It is used for dizziness, insomnia, palpitation, shortness of breath, neurasthenia, exhaustion, cough and asthma. [0003] Ganoderma lucidum is a high-temperature fungus, which mainly grows in humid places. It needs fresh air and a high-humidity growth environment throughout its growth and development. At present, Ganoderma lucidum has been planted in artificial greenhouses. The fruiting bodies (or sclerotia) produced by traditional field collection or artificial cultivation are limited by the scarcity of wild resources, long ...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12Q1/04C12R1/645
CPCC12N1/14C12Q1/04
Inventor 汪琪玮肖建霞闵君萍余敬红刘国斌
Owner 孝感市应用技术研究所
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