PET lytic enzyme mutant, coding gene and application thereof

A technology for mutant and decomposing enzymes, applied in the field of genetic engineering of enzymes, can solve the problems of low absolute degradation rate and inability to degrade hcPET quickly, and achieve the effect of improving enzyme activity
CN106367408AInactive Publication Date: 2017-02-01TIANJIN UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
TIANJIN UNIV
Publication Date
2017-02-01
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a PET (polyethylene terephthalate) lytic enzyme mutant, a coding gene and application thereof. The PET lytic enzyme mutant is represented by an amino acid sequence SEQ ID NO.8 or SEQ ID NO.9 obtained by performing point mutation on an amino acid sequence SEQ ID NO.7. A lytic enzyme gene of escherichia coli is subjected to directed evolution; by site-specific mutagenesis, the PET lytic enzyme mutant is obtained. According to the amount of decomposition products within the same time, the enzyme activity of the PET lytic enzyme mutant is improved.
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Description

technical field

[0001] The invention belongs to the genetic engineering of enzymes, and in particular relates to mutants of PET decomposing enzymes, coding genes and applications thereof. Background technique

[0002] PET (polyethylene terephthalate) has been industrially produced in large quantities for a long time due to its plasticity, transparency and durability. In 2013 alone, the world produced about 56 million tons of PET plastic. Due to the lack of enzymes or extremely low activity that can degrade PET in nature, PET plastics are difficult to be degraded naturally in nature, and become a kind of pollutant, which has a great impact on the environment.

[0003] PET decomposing enzyme ISF6_4831 is a kind of cutinase. Cutinase is a decomposing enzyme that can catalyze the decomposition of ester groups in cutin and produce a large amount of fatty acid monomers. It is a member of the α / β decomposing enzyme family with a smaller molecular weight. The catalytic activity of...

Claims

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